Flav7-loaded Silica-based Hybrid Micelles: Synthesis and Photothermal Performance

doi:10.15541/jim20220240

Abstract

Abstract:

Recently, organic small molecule photothermal agents have great application potentials in the field of biomedicine due to their high extinction coefficient in the near-infrared region and good structural tunability. Unfortunately, most current organic small molecular photothermal agents prove to be still poor water solubility, unsatisfactory biological stability, and low photothermal conversion efficiency. To address these, a simple method was developed to prepare Flav7-loaded silica-based hybrid micelles (FPOMs) for efficient photothermal therapy. Firstly, the Flav7-loaded micelles were prepared by self-assembly of block copolymer PS₁₃₂-b-PAA₁₆. Then, 3-mercaptopropyl trimethoxysilane (MPTMS) and polyethylene glycol (PEG) were introduced to fix the structure and endowed the surface modification of the system, respectively, resulting in the final formation of FPOMs. Results show that FPOMs exhibit excellent photothermal stability and high photothermal conversion efficiency (46.7%) under the excitation of 808 nm laser. Cell experiments indicate that FPOMs have good biocompatibility and photothermal toxicity. It is highly expected to be used as a new type of nano-photothermal agent for efficient and safe photothermal therapy.

Key words: Flav7, silica-based material, micelle, photothermal property

CLC Number:

TQ138

CHI Zheren, ZHANG Liao, GUO Zhiqian, LI Yongsheng, NIU Dechao. Flav7-loaded Silica-based Hybrid Micelles: Synthesis and Photothermal Performance[J]. Journal of Inorganic Materials, 2022, 37(11): 1236-1244.

Figures/Tables 17

Fig. 1 Schematic diagram for the synthesis of Flav7

Fig. 2 Schematic illustration for the fabrication of FPOMs MPTMS: 3-Mercaptopropyl trimethoxsilicon; PEG: Polyethylene glycol; MAL-mPEG: Maleimide-methoxy (polyethylene glycol); PS-b-PAA: Polystyrene-block-polyacrylic acid; FPOMs: Flav7-PEGylated- organosilica-micelles The color figure can be obtained from online edition

Fig. 3 TEM image of Flav7-PEGylated-organosilica-micelles (FPOMs)

Fig. 4 Element mapping scanning images of Flav7-PEGylated- urganosilica-micelles (FPOMs) (a) Image of HAADF; (b-d) Images of O (b), Si (c) and S (d) The color figures can be obtained from online edition

Fig. 5 Hydrodynamic sizes of FMs, FOMs and FPOMs FMs: Flav7-micelles; FOMs: Flav7-organosilica-micelles; FPOMs: Flav7-PEGylated-organosilica-micelles The color figure can be obtained from online edition

Fig. 6 Zeta potentials of FMs, FOMs and FPOMs FMs: Flav7-Micelles; FOMs: Flav7-organosilica-micelles; FPOMs: Flav7-PEGylated-organosilica-micelles The color figure can be obtained from online edition

Fig. 7 FT-IR spectra of FMs, FOMs and FPOMs FMs: Flav7-Micelles; FOMs: Flav7-organosilica-micelles; FPOMs: Flav7-PEGylated-organosilica-micelles

Fig. 8 Absorption spectra of Flav7 (a) and FPOMs (b) in DMF or in water FPOMs: Flav7-PEGylated-organosilica-micelles; DMF: N,N- dimethylformamide

Fig. 9 Hydrodynamic sizes of FMs (a) and FPOMs (b) in H2O, PBS (pH 7.4), RPMI-1640 medium (10% serum) and DMEM medium (10% serum) for a week The color figures can be obtained from online edition

Fig. 10. Absorption spectra of FPOMs under 808 nm laser irradiation for 0-60 min The color figure can be obtained from online edition

Fig. 11 Infrared images of FPOMs under 808 nm laser irradiation

Fig. 12 Photothermal property of FPOMs (a) FPOMs (600 μg/mL) under different power densities of 808 nm laser irradiation; (b) FPOMs at different concentrations under 808 nm (1.0 W/cm2) laser irradiation The color figures can be obtained from online edition

Fig. 13 Photothermal stability of FPOMs and Flav7 in water The color figure can be obtained from online edition

Fig. 14 Photothermal conversion efficiency of FPOMs (1.0 W/cm2, 808 nm) The color figure can be obtained from online edition

Fig. 15 Relative cell viabilities of FPOMs treated cells after laser irradiation at 808 nm (1.0 W/cm2) for 5 min (a) SMMC-7721 cancer cells with or without laser irradiation (***p < 0.001); (b) MEF and 3T3 cells The color figures can be obtained from online edition

Fig. 16 Flow cytometry analysis of SMMC-7721 cells with different treatment and staining by Annexin-V/PI reagents (a) Control; (b) Laser irradiation only; (c) FPOMs only; (d) FPOMs and laser irradiation The color figure can be obtained from online edition

Fig. 17 Confocal laser scanning microscope images of SMMC-7721 cells with FPOMs (0, 150, and 300 μg/mL) treated by (a) or not by (b) laser irradiation (808 nm, 1.0 W/cm2, 5 min). The color figures can be obtained from online edition

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