Photo/Magnetic Thermal Fe2SiO4/Fe3O4 Biphasic Bioceramic and Its Composite Electrospun Membrane: Preparation and Antibacterial

doi:10.15541/jim20210659

Abstract

Abstract:

Biomaterials with both antibacterial and tissue repair activity have promising applications in the field of regenerative medicine. Both photothermal and magnetothermal-based techniques have antimicrobial effects, but the limited penetration capacity of light and the low thermal conversion efficiency of magnetothermal reagents limit their applications in biomedical fields. Here, we synthesized Fe₂SiO₄/Fe₃O₄ biphasic composite bioceramic powders, which not only display good photothermal and magnetothermal effects, but also effectively release active Fe and silicate ions, and prepared electrospun membranes by combining ceramic powders with gelatin/polycaprolactone, which not only exhibit good cytocompatibility, but also, importantly, possess both photothermal and magnetothermal properties. The composite membranes, under conditions of being irradiated with near-infrared light (808 nm, 0.36 W·cm^-2) and placed in alternating magnetic field (506 kHz, 837 A·m^-1) for 15 min simultaneously, are able to inhibit bacterial activity more effectively than the thermal treatment with near-infrared light or magnetic field alone. Therefore, this Fe-Si-based bioceramic and its composite material with photothermal, magnetothermal, antimicrobial, and biocompatible properties, have potential application in the field of regenerative medicine.

Key words: Fe, silicate, biphasic bioceramic, electrospun, antibacterial

CLC Number:

TQ174

SHENG Lili, CHANG Jiang. Photo/Magnetic Thermal Fe₂SiO₄/Fe₃O₄ Biphasic Bioceramic and Its Composite Electrospun Membrane: Preparation and Antibacterial[J]. Journal of Inorganic Materials, 2022, 37(9): 983-990.

Figures/Tables 11

Fig. 1 TG-DTA curves of gel powders tested in (a) air and (b) argon atmosphere

Fig. 2 XRD patterns of FF products calcined at 800 ℃ in different atmospheres FF-1: the product obtained by calcining without argon gas; FF-2-FF-4: the product obtained by calcining with argon gas at 10, 25 and >25 kPa in the furnace, respectively.

Table 1 Ion release of powders prepared under different conditions after 24 h being submersed in cell culture medium ECM

Powder	Fe ion/(μg·mL^-1)	Silicate ion/(μg·mL^-1)
FF-1	43.96	70.12
FF-2	28.37	40.23
FF-3	13.62	27.16
FF-4	5.76	12.68

Fig. 3 Magnetothermal and photothermal properties of powder products after being calcined under different conditions (a) Magnetic analysis results; (b) Results of thermal performance of different powders under alternating magnetic field intensity of 506 kHz at 837 A·m-1; (c) Photothermal performance of different powders under 808 nm near-infrared light irradiation at a density of 0.36 W·cm-2. FF-1: the product obtained by calcining without argon gas; FF-2-FF-4: the product obtained by calcining with argon gas at 10, 25 and >25 kPa in the furnace, respectively. 1 emu=103 A·m-1, 1 Oe=1000/4π A/m Colorful figures are available on website

Fig. 4 Particle size characterization of FF-2 powders

Fig. 5 SEM and TEM images of FF-2 powders (a, b) SEM images at low (a) and high (b) magnification; (c) TEM high-resolution image of the powder with inset showing electron diffraction pattern of the powder

Fig. 6 Morphologies of composite electrospun membranes with different FF-2 powder compositing amounts (a) Optical photos; (b) SEM images. 0, 10, 20, 30, and 40 in the figures represent composite membranes with powder contents of 0, 10%, 20%, 30%, and 40%, respectively

Table 2 Ion release properties of composite electrospun films with different powder contents in cell culture medium ECM

Powder content/%	Fe ion/(μg·mL^-1)	Silicate ion/(μg·mL^-1)
0	0	0
10	0.05	0.27
20	0.13	1.45
30	0.32	2.76
40	0.61	5.98

Fig. 7 Photothermal and magnetothermal properties of composite membranes with different powder contents (a) Under NIR light (0.36 W·cm-2) irradiation at a wavelength of 808 nm; (b) In an alternating magnetic field (506 kHz, 837A·m-1); (c) Composite film with 30% powder content under laser, alternating magnetic field and laser in combination with alternating magnetic field, respectively. 0, 10, 20, 30, and 40 in the figures represent composite membranes with powder contents of 0, 10%, 20%, 30%, and 40%, respectively. MTT: magnetothermal; PTT: photothermal; MTT+PTT: combined photothermal and magnetothermal

Fig. 8 Cytocompatibility of FG-30 composite electrospun membranes (a-f) SEM images of cell adhesion on FG-0 membrane (a-c) and FG-30 composite membrane (d-f) after 24 h of HUVEC culture, respectively; (g) Effect of composite membrane on HUVECs proliferation. Blank, FG-0, and FG-30 in the figures indicate blank control group, FG-0 electrospun membrane group, and FG-30 composite electrospun membrane group, respectively. * indicates p<0.05 which means significant difference between groups

Fig. 9 Combined photo/magneto-thermal inhibition of S.aureus by FG-30 composite membrane (a) Optical photographs of colony coated plates; (b) Statistical results of inhibition rate. Blank, FG-0, FG-30, FG-30 (MTT), FG-30 (PTT), and FG-30 (MTT+PTT) denote blank control, FG-0 membrane, FG-30 membrane, FG-30 membrane with magnetothermal, FG-30 membrane with photothermal, FG-30 membrane with combined photothermal and magnetothermal, respectively. * indicates p<0.05 which means significant difference between groups

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Photo/Magnetic Thermal Fe₂SiO₄/Fe₃O₄ Biphasic Bioceramic and Its Composite Electrospun Membrane: Preparation and Antibacterial

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