Journal of Inorganic Materials ›› 2021, Vol. 36 ›› Issue (11): 1199-1207.DOI: 10.15541/jim20210056
Special Issue: 【虚拟专辑】药物递送(2020~2021)
• RESEARCH ARTICLE • Previous Articles Next Articles
BAO Feng1,2(
), CHANG Jiang1,2()
Received:2021-01-28
Revised:2021-03-24
Published:2021-11-20
Online:2021-04-05
Contact:
CHANG Jiang, professor. E-mail: jchang@mail.sic.ac.cn
About author:BAO Feng(1991-), male, PhD candidate. E-mail: baofeng@student.sic.ac.cn
Supported by:CLC Number:
BAO Feng, CHANG Jiang. Calcium Silicate Nanowires Based Composite Electrospun Scaffolds: Preparation, Ion Release and Cytocompatibility[J]. Journal of Inorganic Materials, 2021, 36(11): 1199-1207.
| Sample | Mass of PCL/g | Mass of PLA/g | Mass of gelatin/g | Volume of HFIP/mL |
|---|---|---|---|---|
| 5ES | 0.175 | 0.175 | 0.150 | 10 |
| 10ES | 0.350 | 0.350 | 0.300 | 10 |
| 18ES | 0.630 | 0.630 | 0.540 | 10 |
| 22ES | 0.770 | 0.770 | 0.660 | 10 |
Table 1 Preparation of electrospun solution with various pore size
| Sample | Mass of PCL/g | Mass of PLA/g | Mass of gelatin/g | Volume of HFIP/mL |
|---|---|---|---|---|
| 5ES | 0.175 | 0.175 | 0.150 | 10 |
| 10ES | 0.350 | 0.350 | 0.300 | 10 |
| 18ES | 0.630 | 0.630 | 0.540 | 10 |
| 22ES | 0.770 | 0.770 | 0.660 | 10 |
| Sample | Voltage/kV | Flow rate / (mL·min-1) | Needle-to-collector distance/cm |
|---|---|---|---|
| 5ES | 10 | 0.02 | 8 |
| 10ES | 12 | 0.05 | 10 |
| 18ES | 15 | 0.08 | 15 |
| 22ES | 18 | 0.20 | 20 |
Table 2 Preparation parameters of electrospun membranes with various pore size
| Sample | Voltage/kV | Flow rate / (mL·min-1) | Needle-to-collector distance/cm |
|---|---|---|---|
| 5ES | 10 | 0.02 | 8 |
| 10ES | 12 | 0.05 | 10 |
| 18ES | 15 | 0.08 | 15 |
| 22ES | 18 | 0.20 | 20 |
Fig. 1 Schematic illustration of different preparation methods for calcium silicate composite electrospun scaffolds with different forms (a) CSH@ES: mixed electrospun of CSH-contained blended electrospun solution; (b) CSH&ES: simultaneously electrospraying CSH particles with elctrospun fibers; (c) CSH/ES: electrospraying CSH particles on the surface of as-spun electrospun membranes; (d) ES/CSH/ES: electrospraying CSH particles on as-spun electrospun membranes, then electrospun fibers on the surface to construct an ES/CSH/ES sandwich structure
Fig. 2 (a) XRD patterns and (b) TEM images of calcium silicate nanowires with inset in (b) showing magnified TEM image of CSH
Fig. 3 SEM images of electrospun scaffolds contained no CSH nanowires with different pore sizes (a) 5ES; (b) 10ES; (c) 15ES; (d) 22ES
| Sample | Fiber diameter/μm | Pore diameter/μm |
|---|---|---|
| 5ES | (0.24±0.05) | (0.68±0.13) |
| 10ES | (0.82±0.16) | (3.45±0.73) |
| 18ES | (4.112±0.81) | (17.73±3.53) |
| 22ES | (10.27±2.04) | (50.46±10.87) |
Table 3 Statistical results of fiber diameter and pore diameter of electrospun scaffolds
| Sample | Fiber diameter/μm | Pore diameter/μm |
|---|---|---|
| 5ES | (0.24±0.05) | (0.68±0.13) |
| 10ES | (0.82±0.16) | (3.45±0.73) |
| 18ES | (4.112±0.81) | (17.73±3.53) |
| 22ES | (10.27±2.04) | (50.46±10.87) |
Fig. 4 SEM images of various forms of calcium silicate composite electrospun scaffolds with different pore sizes and CSH particle distributions in the scaffolds (a, b) CSH@ES; (c, d) CSH&ES; (e, f) CSH//ES; (g, h) ES/CS/ES; (a, c, e, g) Small pore size; (b, d, f, h) Large pore size. The inset in (a) shows CSH nanowires being uniformly distributed inside the fibers. The inset in (c) reveals agglomerated CSH particles being embedded in the scaffolds with white arrows indicating the CSH particles embedded in scaffolds. The inset in (e) shows the CSH nanowires formed agglomerates on the surface of the scaffolds
Fig. 5 Water contact angle measurements of various forms of calcium silicate composite electrospun scaffolds with different pore sizes (a) Photographs of water contact angle on electrospun scaffolds; (b) Statistical results of water contact angle. *p<0.05, ** p<0.01, ***p<0.001
Fig. 6 Effects of the different composite forms on release behavior of SiO32- in calcium silicate nanowires in composite electrospun scaffolds (a) Scaffolds with small pore size; (b) Scaffolds with large pore size
Fig. 7 Schematic illustrations of ions release behaviors from calcium silicate composite in electrospun scaffolds (a) Ions controlled release of CSH@ES and CHS&ES groups; (b) Ions burst release process of CSH//ES and ES/CSH/ES groups
Fig. 8 Proliferation of HUVECs after being cultured on various composite electrospun scaffolds with small pore *p<0.05, ** p< 0.01, ***p<0.001
Fig. 9 HUVEC morphologies after being cultured on various composite electrospun scaffolds with small pore for 1 and 3 d
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