Dentin Remineralization Induced by Micro-nano Bioactive Glass Spheres

doi:10.15541/jim20210158

Abstract

Abstract:

Occlusion of dentine tubules may reduce or eliminate dentin hypersensitivity. Here, micro-nano bioactive glass spheres (MNBGs) with various sizes and sodium alginate-phosphate buffer solution were used to prepare bioactive glass pastes (MNBGP) for dentin desensitization. Through mineralization on the surface of dentin samples in vitro, the bonding ability of different pastes with dentin, as well as the ability to induce dentin remineralization and occlude dentin tubules in vitro, was investigated. The results indicated that the MNBGP could be tightly combined with the dentin interface, and the MNBGs with smaller particle sizes were more uniformly distributed on the surface of the demineralized dentin slices. MNBGP could well mineralize in artificial saliva (AS) to form apatite (HA), induce dentin remineralization, and occlude dentin tubules, which resulted in thickening HA layer, formed on the surface of demineralized dentin slices, increased with the prolonged mineralization time, up to 5-10 μm after 28 d dentin remineralization. The size of bioactive glass microspheres had a certain impact on its ability to induce remineralization. When the particle size was equal to the diameter of the exposed dentin tubules, it could better occlude the dentin tubules. All data indicate that MNBGP has potential application for dentin hypersensitivity treatment.

Key words: dentine hypersensitivity, micro-nano bioactive glass spheres, apatite

CLC Number:

TQ174

TANG Jieyin, WANG Gang, LIU Cong, ZOU Xuenong, CHEN Xiaofeng. Dentin Remineralization Induced by Micro-nano Bioactive Glass Spheres[J]. Journal of Inorganic Materials, 2022, 37(4): 436-444.

Figures/Tables 12

Table 1 Theoretical and measured chemical composition, reagent dosage of MNBGs with different particle sizes

Sample	SiO₂ : CaO : P₂O₅(molar ratio)		DDA/g	TEOS/mL
Sample	Theoretical	Measured	DDA/g	TEOS/mL
MNBGs-1	80 : 16 : 4	87.637 : 12.363 : 0	3	8
MNBGs-2		86.289 : 13.696 : 0.015	6	16
MNBGs-3		89.071 : 10.875 : 0.054	6	24

Fig. 1 SEM (a1-c1), TEM (a2-c2) images and particle size distributions (a3-c3) of MNBGs

Fig. 2 XRD patterns (a) and FT-IR spectra (b) of MNBGs

Table 2 Chemical components (molar percent) and Ca/P ratio on the surface of dentin before and after EDTA etching

Samples	C/%	O/%	Ca/%	P/%	Ca/P
Without etching	0	65.77	20.26	13.97	1.45
With etching	66.82	32.99	0.05	0.14	0.35

Fig. 3 SEM images of the dentin surface without (a) and with (b) EDTA-etching, magnified photo of dentin tubules (insert in (b)), and photo of bioactive glass paste (c)

Fig. 4 SEM images of demineralized dentin slices coating with different MNBGP (a) MNBGP-1; (b) MNBGP-2; (c) MNBGP-3

Fig. 5 ATR-FTIR spectra of demineralized dentin surface before and after coating with MNBGP, and after rinsing with water (a) MNBGP-1; (b) MNBGP-2; (c) MNBGP-3

Fig. 6 SEM images of the surfaces of demineralized dentin slices without (a) (control) and with treatment by MNBGP-1 (b), MNBGP-2 (c), and MNBGP-3 (d) after soaking in AS for 1 d (a1-d1), 7 d (a2-d2), 14 d (a3-d3) and 28 d (a4-d4)

Fig. 7 SEM images of the longitudinal section of demineralized dentin samples without (a) (control) and with treatment by MNBGP-1 (b), MNBGP-2 (c), and MNBGP-3 (d) after soaking in AS for 1 d (a1-d1), 7 d (a2-d2), 14 d (a3-d3) and 28 d (a4-d4)

Fig. 8 EDS analyses of the surface of demineralized dentin slices without (a) (control) and with treatment by MNBGP-1 (b), MNBGP-2 (c), and MNBGP-3 (d) after soaking in AS for 28 d

Table 3 Chemical components ( molar percent) and Ca/P ratio in molar on the surface of remineralized dentin

Sample	Ca/%	P/%	Ca/P
Control	2.49	1.82	1.37
MNBGP-1	16.64	11.69	1.42
MNBGP-2	18.37	12.97	1.42
MNBGP-3	6.16	6.12	1.01

Fig. 9 XRD patterns of the surface of intact dentin, demineralized dentin and slices without treatment (control) and being treated with MNBGP after being soaked in AS for 28 d

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