3D Printing and Characterization of Microsphere Hydroxyapatite Scaffolds

doi:10.15541/jim20200499

Abstract

Abstract:

Surface microstructure of biomaterials plays an important role in osteogenesis. In this study, we prepared a series of hydroxyapatite (HA) scaffolds (HA0, HA10, HA30, HA50) by 3D printing microspheres with different particle sizes (<60 μm). These scaffolds have similar physical and chemical properties, but form different microstructures due to their different particle size of microspheres, which result in certain impact on their biological properties. Compared with the traditional scaffolds without micro-sphere structure (HA0), the microsphere-based hydroxyapatite scaffolds provide improved adhesion and growth sites for bone mesenchymal stem cells (BMSCs) after 24 h culture, among which HA30 scaffold significantly promotes the pseudopodia elongation of BMSCs. After 5 d of cell culture on the scaffolds, the micro-sphere based HA scaffolds significantly stimulate proliferation of the BMSCs in contrast to the HA0 group. HA30 scaffolds with microsphere at similar size to the cell size have the best promoting effect on cell proliferation. Therefore, 3D printing technology can not only control the macrostructure of HA scaffolds, but also construct the microstructure on the surface of HA scaffolds by controlling the particle sizes of bioceramic powders to optimize the biological effects, which shows great potential application in the field of bone tissue engineering.

Key words: hydroxyapatite microspheres, 3D printing, scaffolds, bone tissue engineering

CLC Number:

TQ174

WU Zhongcao, HUAN Zhiguang, ZHU Yufang, WU Chengtie. 3D Printing and Characterization of Microsphere Hydroxyapatite Scaffolds[J]. Journal of Inorganic Materials, 2021, 36(6): 601-607.

Figures/Tables 6

Fig. 1 (a) Optical micrographs and (b) SEM images of HA powders with and without microsphere structure (< 60 μm) ((i) HA0, (ii) HA10, (iii) HA30, (N) HA50), and their (c) particle size statistics after successive sieving

Fig. 2 (a) XRD characterization of HA powder with two morphologies before and after sintered at 1150 ℃, and (b) SEM image with EDS pattern of scaffolds after sintering and grinding.

Fig. 3 (a) 3D printing HA microsphere scaffolds with different particle sizes and microstructure of (b) HA0, (c) HA10, (d) HA30, and (e) HA50 scaffolds

Fig. 4 Accumulative ion release of HA scaffolds

Fig. 5 Growth states of rBMSCs on (a) HA0, (b) HA10, (c) HA30 and (d) HA50 scaffolds with arrows pointing to the cell pseudopodia

Fig. 6 Proliferation of rBMSCs on scaffolds (a) Quantitative statistics of cell proliferation, and (b) fluorescence photograghs of cell proliferation with DAPI staining

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