Preparation of Mesoporous Organosilica-based Nanosystem for in vitro Synergistic Chemo- and Photothermal Therapy

doi:10.15541/jim20200091

Abstract

Abstract:

Organic-inorganic hybrid mesoporous organosilica has gained more attention in biomedicine due to its high surface area, mesoporous channels, functional framework, and high drug loading capacity. In this study, disulfide- bridged hybrid mesoporous organosilica nanoparticles (MONs) as nanocarriers were employed to construct a nanosystem (ICG/DOX-MONs@DNA₂₀) for delivering drugs and photothermal agents, in which DNA molecules as “switches” were modified on the surface of MONs to control drug release. The results showed that the ICG/DOX-MONs@DNA₂₀ nanosystem could increase the temperature to above 43 ℃ for photothermal therapy with near-infrared (NIR) laser irradiation. On the other hand, the ICG/DOX-MONs@DNA₂₀ nanosystem exhibited a very slow release of DOX (12.3% in 6 h) at 37 ℃, but a rapid release of DOX (52.4% in 6 h) occurred at 43 ℃. Cell culture experiments indicated that the nanosystem can be internalized by HeLa cells, and exhibited an enhanced therapeutic efficacy of synergistic chemo- and photothermal therapy. Hence, the ICG/DOX-MONs@DNA₂₀ nanosystem might be promising for synergistic chemo- and photo-thermal tumor therapy.

Key words: mesoporous organosilica, nanocarriers, controlled release, synergistic therapy

CLC Number:

TQ174

ZENG Yulin, CHEN Jiajie, TIAN Zhengfang, ZHU Min, ZHU Yufang. Preparation of Mesoporous Organosilica-based Nanosystem for in vitro Synergistic Chemo- and Photothermal Therapy[J]. Journal of Inorganic Materials, 2020, 35(12): 1365-1372.

Figures/Tables 7

Fig. 1 Characterization of MONs (a) SEM image of MONs; (b) TEM images of MONs; (c) DLS particle size distribution of MONs; (d) N2 adsorption-desorption isotherm and the corresponding pore size distribution of MONs

Fig. 2 (a) Zeta potential, (b) FT-IR spectra and (c) UV-Vis spectra of MONs, MONs-NH2, ICG/DOX-MONs and ICG/DOX-MONs@DNA20 nanoparticles

Fig. 3 Photothermal heating curves of ICG/DOX- MONs@DNA20 suspension with different concentrations under NIR irradiation at 0.75 W/cm2, and (b) photothermal heating curves of 100 μg/mL ICG/DOX-MONs@DNA20 suspension under NIR irradiation at different power densities

Fig. 4 Release profiles of (a) DOX and (b) ICG from ICG/DOX-MONs@DNA20 at 37 and 43 ℃, respectively, and (c) release profile of DOX and ICG from ICG/DOX-MONs@DNA20 at the alternating temperature between 37 and 43 ℃

Fig. 5 Cell viability of HeLa cells cultured with MONs with different concentrations for 24 and 48 h

Fig. 6 Cell uptake of different nanoparticles after being cultured with HeLa cells for 3 h (blue: nuclei; red: DOX)

Fig. 7 Cell viabilities of HeLa cells cultured with ICG/DOX-MONs and ICG/DOX-MONs@DNA20 with or without NIR irradiation *P<0.05, ***P<0.001

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