Fabrication and Fluorescence Biodetection of ZnO Nanorods Using Microfluidic Technology

doi:10.15541/jim20180005

Abstract

Abstract:

A simple microfluidic chip was designed and fabricated, on which zinc oxide (ZnO) nanorods were synthesized by hydrothermal method. The influence of growth condition on morphology and crystal structure of ZnO nanorods were investigated by scanning electron microscope (SEM) and X-ray diffraction (XRD), respectively. Experimental results showed that densely packed ZnO nanorods could be prepared on microfluidic chip, with good crystallinity and c-axis orientation. The diameter and length of ZnO nanorods changed with heating methods and growth time. Moreover, fluorescence detection by ZnO nanorods, synthesized with different heating methods, were evaluated by FITC-labeled goat anti-bovine IgG. And the results revealed that ZnO nanorods synthesized on chip by localized heating showed better detection performance of fluorescein-labeled protein and good linearity from 10 pg/mL to 1 μg/mL with the correlation coefficient of 0.99209. The as-synthesized ZnO nanorods were further used to demonstrate detection of human α-fetoprotein (AFP), and achieved a superior limit of detection as low as 1 pg/mL. This work demonstrated that ZnO nanorods synthesized by microfluidic device allowed multiplexed fluorescence biodetection.

Key words: ZnO, microfluidic chip, fluorescence detection, biosensor

CLC Number:

O611

GUO Ling-Xia, SHI Yu-Chen, ZHAO Zhen-Jie, LI Xin. Fabrication and Fluorescence Biodetection of ZnO Nanorods Using Microfluidic Technology[J]. Journal of Inorganic Materials, 2018, 33(10): 1103-1109.

Figures/Tables 6

Fig. 1 Schematic of microfluidic chip (a), photograph of synthesis of ZnO nanorods on chip by global heating (b) and localized heating (c)

Fig. 2 SEM images of ZnO nanorods grown by global and localized heating method for 0.5 h (a, d), 1 h (b, e) and 3 h(c, f), respectively

Fig. 3 Diameter distribution histograms of ZnO nanorods grown for different time by different heating methods and diameter variation (g) and length variation (h) of ZnO nanorods as a function of growth time (a)-(c): 0.5 h, 1 h and 3 h by global heating; (d)-(f): 0.5 h, 1 h and 3 h by localized heating

Fig. 4 XRD patterns of ZnO nanorods

Fig. 5 Fluorescence images and corresponding detection curve of FITC-anti bovine IgG on ZnO nanorods synthesized by global heating (a-b) and localized heating (c-d)

Fig. 6 Fluorescence images (a) and quantitative analysis of fluorescence intensity with various AFP concentration (b) on ZnO nanowires synthesized by localized heating, as well as fluorescence images (c) and quantitative analysis of fluorescence intensity with AFP detection (d) against other potentially interfering proteins using BSA and PSA Various proteins in each group have the same concentration, 1 μg/mL

References 24

[1]	MANZ A, GRABER N, WIDMER H.Miniaturized total chemical analysis systems: a novel concept for chemical sensing.Sensors and Actuators B: Chemical, 1990, 1(1): 244-248.
[2]	HWANG K, KWON S, JUNG S,et al. Miniaturized bead-beating device to automate full DNA sample preparation processes for gram-positive bacteria. Lab on a chip, 2011,11(21): 3649-3655.
[3]	LING B.Research and industrialization of microfluidic chip.Chinese Journal of Analytical Chemistry, 2016, 44(4): 491-499.
[4]	DHARMASIRI U, NJOROGE S K, WITEK M A,et al. High- throughput selection, enumeration, electrokinetic manipulation, and molecular profiling of low-abundance circulating tumor cells using a microfluidic system. Analytical Chemistry, 2011, 83(6): 2301-2309.
[5]	BWATANGLANG I B, MOHAMMAD F, YUSOF N A.Role of multifunctional nanomaterials in disease diagnosis and therapy.Journal of Chemical and Pharmaceutical Research, 2014, 6(11): 821-844.
[6]	ARYA S K, SAHA S, RAMIREZ-VICK J E,et al. Recent advances in ZnO nanostructures and thin films for biosensor applications: review. Analytica Chimica Acta, 2012, 737(15): 1-21.
[7]	HAHM J I.Zinc oxide nanomaterials for biomedical fluorescence detection.Journal of Nanoscience & Nanotechnology, 2014, 14(1): 475-486.
[8]	FU Y, ZHANG J, LAKOWICZ J R.Photophysical behaviors of single fluorophores localized on zinc oxide nanostructures.International Journal of Molecular Sciences, 2012, 13(9): 12100-12112.
[9]	YIN Y, SUN Y, YU M,et al. ZnO nanorod array grown on Ag layer: a highly efficient fluorescence enhancement platform. Scientific Reports, 2015, 5: 8152-8155.
[10]	WEN Z, WANG G, LI J,et al. Enhanced photocatalytic properties of mesoporous SnO2 induced by low concentration ZnO doping. Crystal Growth & Design, 2007, 7(9): 1722-1725.
[11]	WANG G, CHEN D, LI J,et al. Tunable photocurrent spectrum in well-oriented zinc oxide nanorod arrays with enhanced photocatalytic activity. J. Phys. Chem. C, 2008, 112(24): 8850-8855.
[12]	KIM J, LI Z, PARK I.Direct synthesis and integration of functional nanostructures in microfluidic devices.Lab on Chip, 2011, 11(11): 1946-1951.
[13]	GUO L, SHI Y, LIU X,et al. Enhanced fluorescence detection of proteins using ZnO nanowires integrated inside microfluidic chips. Biosensors & Bioelectronics, 2017, 99: 368-374.
[14]	SHI Y, GUO L, LIU X,et al. Preparation and fluorescence detection property of ZnO nanorods. Micronanoelectronic Technology, 2017, 54(6): 419-425.
[15]	LADANOV M, ALGARINAMARIS P, MATTHEWS G,et al. Microfluidic hydrothermal growth of ZnO nanowires over high aspect ratio microstructures. Nanotechnology, 2013, 24(37): 375301-375309.
[16]	NAM G H, BAEK S H, PARK I K.Growth of ZnO nanorods on graphite substrate and its application for Schottky diode.Journal of Alloys & Compounds, 2014, 613(10): 37-41.
[17]	SCHMIDT-MENDE L, MACMANUS-DRISCOLL J L. ZnO- nanostructures, defects, and devices.Materials Today, 2007, 10(5): 40-48.
[18]	ZHU S, CHEN X, ZUO F,et al. Controllable synthesis of ZnO nanograss with different morphologies and enhanced performance in dye-sensitized solar cells. Journal of Solid State Chemistry, 2013, 197(1): 69-74.
[19]	HAN Z, LI J, HE W, et al. A microfluidic device with integrated ZnO nanowires for photodegradation studies of methylene blue under different conditions. Microelectronic Engineering, 2013, 111(2): 199-203.
[20]	XU C, GAO D.Two-stage hydrothermal growth of long ZnO nanowires for efficient TiO2 nanotube-based dye-sensitized solar cells.Journal of Physical Chemistry C, 2012, 116(12): 7236-7241.
[21]	RICHARDSON J J, LANGE F F.Controlling low temperature aqueous synthesis of ZnO.Crystal Growth & Design, 2009, 9(6): 2570-2575.
[22]	TOPOGLIDIS E, CASS A E G, O'REGAN B,et al. Immobilisation and bioelectrochemistry of proteins on nanoporous TiO2, and ZnO films. Journal of Electroanalytical Chemistry, 2001, 517(1/2): 20-27.
[23]	FU Y, ZHANG J, LAKOWICZ J R.Photophysical behaviors of single fluorophores localized on zinc oxide nanostructures.International Journal of Molecular Sciences, 2012, 13(9): 12100-12112.
[24]	BÖRNER S, RÜTER C E, VOSS T,et al. Modeling of ZnO nanorods for evanescent field optical sensors. Physica Status Solidi, 2007, 204(10): 3487-3495.