Preparation and Antibacterial Activity of Chitosan/Organic Laponite Nanocomposites

doi:10.15541/jim20150509

Abstract

Abstract:

Laponite-based secondary intercalation compound was prepared by ion exchange method through intercalating quaternary ammonium salts and chitosan into the layers of hectorite. The layer spacing of the two kinds of secondary intercalation compounds were increased 0.276 nm and 0.262 nm, respectively, compared to the pure laponite. The antibacterial of secondary intercalation compound was tested by Escherichia coli (E. coli) and Staphylococcus aureus (S. cereus). Antibacterial ability of the two kinds of secondary intercalation compounds were determined by using bacteriostatic ring and plate count methods. The results showed that, after being contacted with the two compounds for 24 h, the bacteriostatic rate of the laponite/myristyl trimethyl ammonium bromide/chitosan on E. coli reached up to 100% and 85% on S.cereus. Meanwhile, the laponite/cetyl trimethyl ammonium bromide/chitosan was up to 99% on E. coli and S. cereus. The antibacterial process and mechanism on E. coli and S. cereus of the two kinds of compounds were investigated by scanning electron microscope and β-galactosidase activity. The results showed that the compound adsorbed bacterial through the role of mutual attraction between positive and negative charges at first, then the compound penetrated the bacterial cell membrane with the help of hydrophobic interaction of organic cation, and finally exerted the sterilization effect.

Key words: organic laponite, chitosan, antibacterial mechanism, secondary intercalation, nanocomposites

CLC Number:

TB33

FU Hai-Li, ZHANG Wen, ZHANG Hua, SONG Shao-Bo, LI Wei. Preparation and Antibacterial Activity of Chitosan/Organic Laponite Nanocomposites[J]. Journal of Inorganic Materials, 2016, 31(5): 479-484.

Figures/Tables 9

Fig. 1 Preparation mechanism of organic laponite nanocomposites

Fig. 2 XRD pattems of laponite and chitosan/modified laponite (a) LAP; (b) LTTC; (c) LCTC

Fig. 3 SEM (a,b) and TEM (c,d) images of laponite and chitosan/modified laponite

Fig. 4 Antimicrobial activity of LTT (A, A’), LCT (B, B’), LC (C, C’), LTTC (D, D’) and LCTC (E, E’) against E. coli and S. aureus

Table 1 Bactericidal ratio of LTTC and LCTC

Samples	E. coli		S.cereus
Samples	Recovered bacteria count /(cfu•mL^-1)	Antimicrobial ratio /%	Recovered bacteria count /(cfu•mL^-1)	Antimicrobial ratio /%
Blank	2.59×10⁸	0	3.40×10⁹	0
LTTC	0	100.0	5.00×10⁸	85.3
LCTC	1.10×10⁴	99.9	1.00×10⁴	99.9
Positive control	2.30×10⁴	99.9	1.00×10³	99.9
Negative control	3.30×10⁸	0	2.86×10⁹	15.9

Fig. 5 SEM images of chitosan/modified laponite after treatment with E.coli (a-d) and S.cereus (a’-d’)

Table 2 Zeta potential of LAP, LTTC and LCTC (n=3)

Sample	Static dissipative	Zeta potential/mV
LAP	-	-27.6±4.8
LTTC	+	43.0±1.6
LCTC	+	82.3±2.1

Fig. 6 Comparison of S.cereus (a-c) and E. coli (a’-c’) activity under different samples (a) Blank; (b) LTTC; (c) LCTC

Fig. 7 Change of permeability of bacterial inner membrane under the condition of chitosan/modified laponite (a) LTTC; (b)LCTC; (c) Blank

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