Magnesium Phosphate/PBS/Wheat Protein Biocomposite for Bone Repair

doi:10.15541/jim20150076

Abstract

Abstract:

Magnesium phosphate was prepared through coprecipitation method, and biocomposite containing magnesium phosphate(MP), polybutylene succinate (PBS) and wheat protein (WP) was fabricated. In vitro degradability, bioactivity and cell responses to MP/PBS/WP composite were investigated. The results showed that the pH value changed from 7.4 to 7.51 after the MP/PBS/WP composite being soaked in Tris-HCl buffer solution for 10 d. The weight loss of the composite reached 58.43wt% after soaking for 12 w. Apatite layer could form on the composite surfaces after soaking in SBF solution for 10 d, indicating good bioactivity. In addition, the MP/PBS/WP composite could promote proliferation and differentiation of MC3T3-E1 cells. All data from this study show that the MP/PBS/WP composite has good degradability, bioactivity and cytocompatibility, showing a potential to be used as a new biomaterial for bone repair.

Key words: magnesium phosphate, PBS, wheat protein, biocomposite, bone repair

CLC Number:

TB383

WANG Quan-Xiang, WU Ying-Yang, DONG Xie-Ping, MA Xu-Hui, WEI Jie. Magnesium Phosphate/PBS/Wheat Protein Biocomposite for Bone Repair[J]. Journal of Inorganic Materials, 2015, 30(9): 957-962.

Figures/Tables 8

Fig. 1 TEM image of magnesium phosphate (a) and photos of PBS, MP/PBS composite and MP/PBS/WP composite (b)

Fig. 2 XRD patterns of PBS (a), MP/PBS composite (b), MP/PBS/WP composite (c), MP (d), and WP (e)

Fig. 3 Weight loss (A) and pH change (B) of PBS (a), MP/PBS composite (b) and MP/PBS/WP composite (c) soaked in Tris-HCl solution for different time

Fig. 4 SEM images of surface morphology of PBS (a), MP/PBS composite (b), and MP/PBS/WP composite (c) after soaking in SBF for 10 d

Fig. 5 EDS spectra of MP/PBS composite and MP/PBS/WP composite after soaking in SBF for 10 d

Fig. 6 Ion concentrations changes in SBF after MP/PBS composite (a) and MP/PBS/WP composite (b) immersion for different time

Fig. 7 CLSM of cytoskeleton stained by FITC and DAPI after MC3T3-E1 cells cultured on PBS (a), MP/PBS composite (b) and MP/PBS/WP composite (c) for 7 d

Fig. 8 Cell proliferation (A) and ALP activity (B) of MC3T3-E1 cells cultured on PBS (a), MP/PBS composite (b) and MP/PBS/WP composite (c) for different time

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