Effect of Surface Topography of Hydroxyapatite on Human Osteosarcoma MG-63 Cell

doi:10.3724/SP.J.1077.2013.13058

Abstract

Abstract: The effect of surface topography of hydroxyapatite (HA) on Human osteosarcoma MG-63 cell lines response was investigated. HA discs with various pore sizes, different pore morphology and distribution were produced by single-axis pressing method. Cell morphology and proliferation were evaluated by scanning electron microscope (SEM) and MTT methods, respectively, and the differentiation potential was assessed by alkaline phosphatase (ALP) activity testing and osteogenic gene expression (RT-PCR). The results showed that the HA discs with macroporous structure (>200 μm) would promote cell adhesion and proliferation, while osteogenic differentiation of MG-63 cells was facilitated by the HA discs with micropore structure (<100 μm). Moreover, the differentiation was also influenced by regularity and distribution of the pore. The irregular and alveolate micropore showed a stronger ability of osteogenic induction than smooth and shallow micropore structure.

Key words: hydroxyapatite, surface topography, osteoinductivity, MG-63 cell

CLC Number:

R318
TB321

CHEN Xiao-Qin, CHEN Xue-Ning, ZHU Xiang-Dong, CAI Bing, FAN Hong-Song, ZHANG Xing-Dong. Effect of Surface Topography of Hydroxyapatite on Human Osteosarcoma MG-63 Cell[J]. Journal of Inorganic Materials, 2013, 28(8): 901-906.

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