Effect of Interaction of Ni2+ and Green Tea Epigallocatechin Gallate on Tongue Carcinoma Cells

doi:10.3724/SP.J.1077.2012.00179

Abstract

Abstract:

Ni²⁺ is the major ion release from nickel-based dental casting alloy and carcinogens. The major polyphenolic constituent present in green tea, (-)epigallocatechin-3-gallate (EGCG), shows chemoprevention on human cancer. The aim of this study was to determine the interaction between EGCG and Ni²⁺ and the biological effects on tongue squamous cell carcinoma. The interaction of EGCG and Ni²⁺ was analyzed by LC/MS. The cell viability was assessed by the MTT reduction assay. The results of LC/MS indicated that [EGCG-2Ni²⁺] and [EGCG-Ni²⁺] formed when EGCG and Ni²⁺ were added simultaneously. Ni²⁺ and EGCG both inhibited the proliferation of tongue carcinoma cells in a time- and dose-dependent manner. The cytotoxicity against tongue carcinoma cells was increased when EGCG and Ni²⁺ cotreated, and the inhibition effects were extremely dependent on the concentrations of EGCG and Ni²⁺, addition order and the ratio of [Ni²⁺] to [EGCG].

Key words: dental casting alloy, green tea, Ni²⁺, EGCG: tongue carcinoma cells

CLC Number:

R783

LUO Zhen-Lan, WANG Hai-Bo, SU Jian-Sheng. Effect of Interaction of Ni²⁺ and Green Tea Epigallocatechin Gallate on Tongue Carcinoma Cells[J]. Journal of Inorganic Materials, 2012, 27(2): 179-184.

Figures/Tables 7

Fig. 1 LC/MS analysis of the interaction between Ni2+ and EGCG in the negative ion mode

Fig. 2 Construction of complexes of EGCG and Ni2+

Fig. 3 Cytotoxicity of EGCG concentration(50-500 μmol/L) against tongue carcinoma cells

Fig. 4 Cytotoxicity of Ni2+ concentration(50-1000 mol/L) against tongue carcinoma cells

Fig. 5 Effects of EGCG and Ni2+ concentrations on the cytotoxicity of EGCG against tongue carcinoma cells

Fig. 6 Effect of the addition order of EGCG and Ni2+ on the viability of tongue carcinoma cells

Fig. 7 Effects of the ratio of Ni2+ to EGCG on the viability of tongue carcinoma cells cells

References 24

[1]	López-Alías J F, Martinez-Gomis J, Anglada J M, et al. Ion release from dental casting alloys as assessed by a continuous flow system: nutritional and toxicological implications. J. Dent. Mater., 2006, 22(9): 832-837.
[2]	Nagle D G, Ferreira D, Zhou Y D. Epigallocatechin-3-gallate (EGCG): chemical and biomedical perspectives. Phytochemistry, 2006, 67(17):1849-1855.
[3]	Thomas R, Kim M H. Epigallocatechin gallate inhibits HIF-1 degradation in prostate cancer cells. Biochem. Biophs. Res. Commun., 2005, 334(2): 543-548.
[4]	Nishikawa T, Nakajima T, Moriguchi M, et al. A green tea polyphenol, epigalocatechin-3-gallate, induces apoptosis of human hepatocellular carcinoma, possibly through inhibition of Bcl-2 family proteins. J. Hepatol., 2006, 44(6): 1074-1082.
[5]	Annabi B, Currie J C, Moghrabi A, et al. Inhibition of HuR and MMP-9 expression in macrophage-differentiated HL-60 myeloid leukemia cells by green tea polyphenol EGCG. Leuk Res., 2007, 31(9): 1277-1284.
[6]	Noda C, He J, Takano T, et al. Induction of apoptosis by epigallocatechin-3-gallate in human lymphoblastoid B cells. Biochem. Biophs. Res. Commun., 2007, 362(4): 951-957.
[7]	Thangapazham R L, Singh A K, Sharma A, et al. Green tea polyphenols and its constituent epigallocatechin gallate inhibits proliferation of human breast cancer cells in vitro and in vivo. Cancer Lett., 2007, 245(1/2): 232-241.
[8]	Hsu S D, Yamamoto T, Borke J, et al. Green tea polyphenol-induced epidermal keratinocyte differentiation is associated with coordinated expression of p57/KIP2 and caspase 14. J. Pharmacol. Exp. Ther., 2005, 312(3): 884-890.
[9]	Babich H, Krupka M E, Nissim H A, et al. Differential in vitro cytotoxicity of (-)-epicatechin gallate (ECG) to cancer and normal cells from the human oral cavity. Toxicol. Vitro, 2005, 19(2): 231-242.
[10]	Yamamoto T, Digumarthi H, Aranbayeva Z, et al. EGCG-targeted p57/KIP2 reduces tumorigenicity of oral carcinoma cells: role of c-Jun N-terminal kinase. Tox Appl. Ph., 2007, 224(3): 318-325.
[11]	Furukawa A, Oikawa S, Murata M, et al. (-)-Epigallocatechin gallate causes oxidative damage to isolated and cellular DNA. Biochem. Pharmacol., 2003, 66(9): 1769-1778.
[12]	Azam S, Hadi N, Khan N U, et al. Prooxidant property of green tea polyphenols epicatechin and epigallocatechin-3-gallate: implications for anticancer properties. Toxicol. Vitro, 2004, 18(5): 555-561.
[13]	Zhang L C, Yu H N, Sun S L, et al. Investigations of the cytotoxicity of epigallocatechin-3-gallate against PC-3 cells in the presence of Cd2+ in vitro. Toxicol Vitro, 2008, 22(4): 953-960.
[14]	Navarro R E, Santacruz H, Inoue M. Complexation of epigallocatechin gallate (a green tea extract, EGCG) with Mn2+: nuclear spin relaxation by the paramagnetic ion. J. Inorg. Biochem., 2005, 99(2): 584-588.
[15]	Esparza I, Salinas I, Santamara C, et al. Electrochemical and theoretical complexation studies for Zn and Cu with individual polyphenols. Anal. Chim. Acta, 2005, 543(2): 267-274.
[16]	苏俭生, 罗真兰, 王海波. Ni2+与EGCG相互作用对牙龈成纤维细胞的生物学效应. 复旦学报, 2001, 37(5): 519-525.
[17]	Yu H N, Yin J J, Shen S R. Growth inhibition of prostate cancer cells by epigallocatechin gallate in the presence of Cu2+. J. Agric Food Chem., 2004, 52(3): 462-466.
[18]	Yu H N, Yin J J, Shen S R. Effects of epigallocatechin gallate on PC-3 cell cytoplasmic membrane in the presence of Cu2+. Food Chem., 2006, 95(1): 108-115.
[19]	Chen X, Yu H N, Shen S G, et al. Role of Zn2+ in epigallocatechin gallate affecting the growth of PC-3 cells. J. Trace. Elem. Med. Biol., 2007, 21(2): 125-131.
[20]	Messer R L, Bishop S, Lucas L C. Effects of metallic ion toxicity on human gingival fibroblasts morphology. Biomaterials., 1999, 20(18): 1647-1657.
[21]	Garceau N, Pichaud N, Couture P. Inhibition of goldfish mitochondrial metabolism by in vitro exposure to Cd, Cu and Ni. Aquat Toxicol., 2010, 98(2):107-112.
[22]	Cangul H, Broday L, Salnikow K, et al. Molecular mechanisms of nickel carcinogenesis. Toxicol Lett., 2002, 127(1): 69-75.
[23]	Kasprzak K S, Sunderman F W, Salnikow K. Nickel carcinogenesis. Mutat. Res., 2003, 533(1): 67-97.
[24]	Yu H N, Shen S R, Yin J J. Effects of interactions of EGCG and Cd2+ on the growth of PC-3 cells and their mechanisms. Food Chem. Toxicol, 2007, 45(2): 244-249.

Effect of Interaction of Ni²⁺ and Green Tea Epigallocatechin Gallate on Tongue Carcinoma Cells

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