Properties of Calcium Phosphate Cement with various Concentrations of Xiangdan Injection

doi:10.3724/SP.J.1077.2010.00507

Abstract

Abstract:

Effects of the concentrations of Xiangdan injection on properties of calcium phosphate cement（CPC） and the drug release rate were investigated, which would offer the theory evidence for optimizing the concentration of Xiangdan in CPC. Xiangdan and calcium hydrogen phosphate (DCPD) were mixed and dried to prepare Xiangdanloaded DCPD which was subsequently used to prepare CPCs containing various concentrations of Xiangdan. The properties of CPCs with various Xiangdan concentrations were characterized by Gilmore needles, universal material mechanical machine, Xray diffraction (XRD), Fouriertransform infrared spectrometer (FT-IR), and Scanning electron microscope (SEM). The in vitro drug release was studied. The setting time increased with increasing Xiangdan concentration in CPC, while the setting time at a concentration of or below 0.2mL/g could meet the clinic requirement. The compressive strength increased with increasing Xiangdan concentration in CPC. No significant difference in the phase component and conversion degree was observed in the hydrated CPC with and without Xiangdan. SEM tests found that the morphology of crystals changed from particles to plates with the increase of Xiangdan concentration in CPC. In the first 4h, the in vitro release of Xiangdan from CPC with a Xiangdan concentration of 0.1-0.5mL/g could satisfy with the clinic requirement. It can be concluded that both the properties of CPC and the in vitro release of Xiangdan from CPC could meet the clinical applications in the primary stage when Xiangdan concentrations were in the range of 0.1-0.2mL/g.

Key words: calcium phosphate cement, xiangdan injection, property, concentration, drug release

CLC Number:

TB321
R944

LI Mao-Hong1,2, QU Shu-Xin2, YAO Ning2,3, GUO Yue-Hua2, ZHANG Tao2, WENG Jie2. Properties of Calcium Phosphate Cement with various Concentrations of Xiangdan Injection[J]. Journal of Inorganic Materials, 2010, 25(5): 507-511.

References

[1]Ginebra M P, Traykova T, Planell J A. Calcium phosphate cements as bone drug delivery systems: a review.J. Control Release,2006, 113(2):102-110
[2]时述山, 胥少汀. 实用骨与软骨移植. 北京：人民军医出版社, 2002: 189.
[3]黄兆胜, 华碧春, 冼建春. 骨伤中草药与验方. 福州：福建科学技术出版社, 2004: 6-182.
[4]刘向前. 骨伤科常用中药配伍运用. 北京：人民军医出版社, 2007: 251-318.
[5]Lin F H, Dong G C, Chen K S, et al. Immobilization of Chinese herbal medicine onto the surface-modified calcium hydrogenphosphate.Biomaterials,2003, 24(3):2413-2422
[6]任鹏宇, 刘娟, 刘彦宁, 等. 丹红注射液磷酸钙骨水泥释放体系的力学性能、显微结构及体外释放. 中国组织工程研究与临床康复, 2009, 13(3): 487-492.
[7]姜红江, 黄相杰, 谭远超, 等. 磷酸钙骨水泥复方丹参缓释体的制备及性能评价.中国修复重建外科杂志,2007, 21(10):1113-1117
[8]Qu S X, Weng J, Feng B, et al. Preliminary study of calcium phosphate immobilized with Chinese medicine.J. Mater. Sci,2005, 40(11):3035-3037
[9]Li M H, Qu S X, Shen R, et al. Study on synthesis of calcium phosphate biomaterials containing SMB. Key Eng. Mat., 2007, 330332(2): 1033-1036.
[10]李茂红, 屈树新, 姚宁, 等. 含丹参磷酸钙骨水泥的制备和表征.生物医学工程与临床,2005, 9(3):132-135
[11]Liu Y R, Qu S X, Maitz M F, et al. The effect of the major components of Salvia Miltiorrhiza Bunge on bone marrow cells.J. Ethnopharmacol,2007, 111(3):573-583
[12]Panzavolta S, Torricelli P, Bracci B, et al. Alendronate and Pamidronate calcium phosphate bone cements: setting properties and in vitro response of osteoblast and osteoclast cells.J. Inorg. Biochem,2009, 103(1):101-106
[13]Young A M, Ng P Y J, Gbureck U, et al. Characterization of chlorhexidine-releasing, fast-setting, brushite bone cements.Acta. Biomater,2008, 4(4):1081-1088
[14]Khairoun I, Driessens F C M, Boltong M G, et al. Addition of cohesion promotors to calcium phosphate cements.Biomaterials,1999, 20(4):393-398
[15]黄粤, 刘昌胜, 邵慧芳, 等. 妥布霉素对磷酸钙骨水泥性能的影响. 中国生物医学工程学报, 2002, 21(5): 417-421.
[16]Ginebra M P, Fernández E, Boltong M G, et al. Compliance of an apatitic calcium phosphate short-term cement with the short-time clinicl requirments in bone surgery, orthopaedics and dentistry.Clin. Mater,1994, 17(2):99-104
[17]郜成莹, 叶建东. 磷酸钙骨水泥负载庆大霉素的制备与表征.材料导报,2008, 22(3):151-154
[18]Dos Santos L A, de Olibeira L C, Rigo E C S. Influence of polymeric additives on the mechanical properties of αtricalcium phosphate cement.Bone,1999, 25(2Suppl):99-S
[19]Takechi M, Miyamoto Y, Ishikawa K, et al. Effects of added antibiotics on the basic properties of antiwashouttype fastsetting calcium phosphate cement.J. Biomed. Mater. Res,1998, 39(2):308-316
[20]浙江大学化学系分析化学教研室. 分析化学手册第一分册. 北京：化学工业出版社, 1979: 55.
[21]Doi Y, Shimizu Y, Moriwaki Y, et al. Development of a new calcium phosphate cement that contains sodium calcium phosphate.Biomaterials,2001, 22(8):847-854
[22]Yokoyama Atsuro, Yamamoto Satoru, Kawasaki Takao, et al. Development of calcium phosphate cement using chitosan and citric acid for bone substitute materials.Biomaterials,2002, 23(4):1091-1101
[23]宋志国, 周大利, 尹光福, 等. 含盐酸四环素αTCP骨水泥的理化性能.功能材料,2004, 35(1):111-113
[24]邵慧芳, 刘昌胜, 黄粤, 等(SHAO Hui-Fang, et al). 羟基磷灰石晶种的形貌控制及其对磷酸钙骨水泥的原位增强研究. 无机材料学报(Journal of Inorganic Materials), 2001, 16(5): 933-939.
[25]王文魁, 王继扬, 赵珊茸. 晶体形貌学. 武汉: 中国地质大学出版社, 2001: 120.
[26]刘玉荣. 多孔磷酸钙骨水泥及丹参/磷酸钙骨水泥复合物的研究. 成都: 西南交通大学硕士论文, 2006.