Inhibitory Effect of Nano-HA Particles on Human U87 Glioblastoma Cells Viability

doi:10.3724/sp.j.1077.2009.09246

Abstract

Abstract: To evaluate the effects of Hydroxyapatite nano-particles (nano-HA) with different morphologies on human U87 glioblastoma cell line (U87), three kinds of nano-HA were designed and synthesized, and a co-culture system containing nano-HA and U87 cells was established. Polyethylene glycol (PEG) was also added to the HA medium system for the samples stabilization. After determining the stabilization and high dispersed nanometer size of nano-HA in the medium system using Transmission electron microscope (TEM) and dynamic light scattering (DLS), the effects of nano-HA on GBM cells were assessed with an MTT method. Results showed that nano-HA could effectively inhibit U87 cells proliferation. Compared with other nano-HA, the needle-like nano-HA were more powerful in inhibiting U87 cells proliferation. Toll-like receptor 4 (TLR4) induced by different nano-HA may be one reason for their different bioactivity, however, the correlation between nano-HA and TLR4 expression needs further investigation.

Key words: hydroxyapatite, glioblastoma, toll-like receptor 4, polyethylene glycol.

CLC Number:

R739
TB321

TIAN Ang,WANG Chao,XUE Xiang-Xin,WU Anhua,GUAN Ge-Fei,WANG Li,QIU Bo. Inhibitory Effect of Nano-HA Particles on Human U87 Glioblastoma Cells Viability[J]. Journal of Inorganic Materials, DOI: 10.3724/sp.j.1077.2009.09246.

References

[1]Bondy M L, Scheurer M E, Malmer B, et al. Cancer, 2008.113(7):1953-1968.
[2]Stupp R, Mason WP, Van Den Bent MJ, et al. N. Engl. J. Med, 2005.352 (10):987-996.
[3]Bauer I W, Li S P, Han Y C, et al. J. Mater. Sci: Mater Med, 2008.19(3):1091-1095.
[4]Murugan R, Ramakrishna S. Compos. Sci. Technol, 2005.65(15-16):2385-2406
[5]Li J , Yin Y, Yao F, et al. Mater. Lett, 2008.62(17-18): 3220-3223.
[6]Fu Q, Zhou N, Huang W H, et al. J. Biomed. Mater. Res. A, 2005.74(2):156-163.
[7]Yin M Z, Han Y C, Bauer I W, et al. Biomed. Mater, 2006.1(1): 38-41.
[8]Shi Z L, Huang X, Cai Y, et al. Acta. Biomaterialia, 2009.5(1):338-345.
[9]Cai Y R, Liu Y K, Yan W Q, et al. J. Mater. Chem, 2007.17(36):3780-3787.
[10]Li B, Guo B, Fan H S, et al. Appl. Surf. Sci, 2008.255(2):357-360.
[11]Yu L, Chen S. Cancer. Immunol. Immunother, 2008.57(9):1271-1278.
[12]Guo X Y, Gough J E, Xiao P, et al. J. Biomed. Mater. Res. A, 2007.82A(4):1022-1032.
[13]Yousefpour M, Afshar A, Yang X D, et al. J. Ectroanal. Chem, 2006. 589(1):96–105.
[14]Ma M H, Ye W, Wang X X. Mater. Lett, 2008.62(23):3875-3877.
[15]Cengiz B, Gokce Y, Yildiz N, et al. Colloids. Surfaces. A, 2008.322(1-3):29-33.
[16]Yin M Z, Han Y C, Dai H L, et al. J. Wuhan. Univ. Technol, 2006.21(4):102-104.
[17]Grandjean-Laquerriere A, Laquerriere P, Laurent-Maquin D, et al. Biomaterials, 2004.25(28): 5921-5927.
[18]Grandjean-Laquerriere A, Laquerriere P, Guenounou M, et al. Biomaterials, 2005.26(15):2361-2369.
[19]Grandjean-Laquerriere A, Tabary O, Jacquot J, et al. Biomaterials, 2007.28(3):400-404.
[20]Kundu S D, Lee C, Billips B K, et al. Prostate, 2008.68(2):223-229.
[21]Jego G, Bataille R, Geffroy-Luseau A, et al. Leukemia, 2006.20(6):1130-1137.
[22]Chochi K, Ichikura T, Kinoshita M, et al. Clin. Cancer. Res, 2008.14(10):2909-2917.