Study on Covalent Immobilization of Heparin on Ti-O-N Surface and Antithrombogenicity

doi:10.3724/SP.J.1077.2007.00996

Abstract

Abstract: Fourier transform infrared spectroscope (FTIR) and X-ray photoelectron spectroscope (XPS) were used to characterize the surface component and bonding state, and these analyses show that the content of hydroxyl group on the surface of Ti-O-N film is enhanced by the chemisorption of phosphoric acid, and the reaction sites with 3-aminopropyltriethoxysilane (APTES) are amplified, furthermore the concentration of immobilized heparin molecules are increased. Fluorescence staining analysis qualitatively proves that the terminal amino groups of APTES are in existence. The result of the toluidine blue method shows that the concentrations of the immobilized heparin on the Ti-O-N film treated by phosphoric acid are 6.6μg/cm². The evaluation of the platelet adhesion test in ~vitro indicates that the Ti-O-N film treated by phosphoric acid and then immobilized with the heparin molecules can effectively suppress the adhesion and activation of the platelets, and has a better antithrombotic property.

Key words: Ti-O-N film, chemisorption of phosphate, covalent immobilization of heparin, antithrombogenicity

CLC Number:

TB34
TB43

HOU Rui-Xia,WENG Ya-Jun,WANG Jin,WANG Zhi-Hao,LENG Yong-Xiang,HUANG Nan. Study on Covalent Immobilization of Heparin on Ti-O-N Surface and Antithrombogenicity[J]. Journal of Inorganic Materials, DOI: 10.3724/SP.J.1077.2007.00996.

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