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无机材料学报

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纳米TiO2光诱导杀伤 Bel-7402人体肝癌细胞研究

夏春辉1, 王百齐2, 王玉1,3, 刘亚琴1, 徐广有1, 吴山1   

  1. 1. 齐齐哈尔医学院化学教研室, 齐齐哈尔 161042; 2. 哈尔滨工业大学应用化学系, 哈尔滨 150001; 3. 中国医科大学生物化学教研室, 沈阳 110001
  • 收稿日期:2005-12-19 修回日期:2006-03-16 出版日期:2006-11-20 网络出版日期:2006-11-20

Damaging Effects of Nanosized TiO2 on Bel-7402 Human Liver Cancer Cell Under Photoinduce

XIA Chun-Hui1, WANG Bai-Qi2, WANG Yu 1,3, LIU Ya-Qin1, XU Guang-You1, WU Shan1   

  1. 1. Chemistry Department, Qiqihaer Medical College, Qiqihaer 161042, China;
    2. Department of Applied Chemistry, Harbin Institute of Technology, Harbin 150001, China; 3. Department of Biological Chemistry, China Medical University, Shenyang 110001, China
  • Received:2005-12-19 Revised:2006-03-16 Published:2006-11-20 Online:2006-11-20

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摘要： 在光诱导条件下, 采用HE染色法和四甲基偶氮唑蓝比色法(MTT法), 研究了纳米TiO2对Bel-7402人体肝癌细胞的杀伤作用, 考察了在不同纳米TiO2浓度、不同光照时间下纳米TiO2的抑瘤效果, 并且对抑瘤机制进行了探讨. 结果发现: 在光诱导条件下, 适宜的TiO2浓度具有较高的抑瘤率, 同时抑瘤过程表现出类似一级反应的动力学规律; 在光诱导条件下, 纳米TiO2产生的活性氧组分与癌细胞膜内外的生物大分子反应, 引起广泛的细胞结构破坏; 造成癌细胞内Ca 2+离子稳态失去平衡; 引发细胞微管相关蛋白2(MAP-2)表达的变化, 促进微管发生重组, 从而导致细胞凋亡和坏死.

关键词: 纳米TiO2, 光诱导杀伤, 肝癌细胞, MAP-2

Abstract: The damaging effects of nanosized
TiO2 on human Bel-7402 liver cancer cell were investigated by means of HE
dye method and MTT colorimetric assay under condition of photoinduce. The
influences of nanosized TiO2 content, and irradiation time on cancer inhibition were systematically studied, and the inhibition mechanism was primarily discussed. The results show that nanosized TiO2 exhibits good inhibition effects under appropriate TiO2 concentration, and the inhibition process obeys first order reaction rule approximately; In addition, activity oxygen produced by nanosized TiO2 under illumination can react with organicmolecules in and out the cancer cell membrane, so that lead to damage cell structure, result into imbalance of Ca 2+ in the cancer cell, and induce microtubule reassembly due to expression changes of microtubule-associated protein-2, which are responsible for the apoptotic and dead cancer cells.

Key words: nanosized TiO2, photoinduced-damage, liver cancer cell, MAP-2

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