三种临床盖髓剂的抗菌性及生物相容性对比研究

doi:10.15541/jim20220465

无机材料学报 ›› 2023, Vol. 38 ›› Issue (12): 1449-1456.DOI: 10.15541/jim20220465

所属专题：【生物材料】骨骼与齿类组织修复(202312)

三种临床盖髓剂的抗菌性及生物相容性对比研究

谢家晔¹(), 李力文¹, 朱强²()

1.上海交通大学医学院附属同仁医院, 口腔科, 上海 200336
2.海军军医大学第一附属医院(长海医院), 口腔科, 上海 200433

收稿日期:2022-08-05 修回日期:2023-06-30 出版日期:2023-07-28 网络出版日期:2023-07-28
通讯作者: 朱强, 副教授. E-mail: txzdzq1979@163.com
作者简介:谢家晔(1979-), 男, 硕士研究生. E-mail: xpony171207@163.com
基金资助:
上海市卫生和计划生育委员会科研课题(201840040)

Contrastive Study on in Vitro Antibacterial Property and Biocompatibility of Three Clinical Pulp Capping Agents

XIE Jiaye¹(), LI Liwen¹, ZHU Qiang²()

1. Department of Stomatology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China
2. Department of Stomatology, The First Affiliated Hospital of Naval Medical University (Changhai Hospital), Shanghai 200433, China

Received:2022-08-05 Revised:2023-06-30 Published:2023-07-28 Online:2023-07-28
Contact: ZHU Qiang, associate professor. E-mail: txzdzq1979@163.com
About author:XIE Jiaye (1979-), male, Master candidate. E-mail: xpony171207@163.com
Supported by:
Scientific Research Project of Shanghai Health and Family Planning Commission(201840040)

摘要/Abstract

摘要：

盖髓剂对于保存牙髓和治疗龋病具有重要作用。临床常用的三种盖髓剂的治疗效果存在较大差异, 如何依据病情选择恰当的盖髓剂亟需相关对比研究予以指导。本研究旨在对临床常用的三种盖髓剂: 齿科氧化锌丁香酚水门汀(ZnO)、自固化氢氧化钙(Dycal)和光固化氢氧化钙间接盖髓剂(Calcimol)的形貌、成分以及理化性能进行表征, 并通过体外细菌和细胞培养实验对其抗菌性和生物相容性进行对比评价。实验结果表明, 齿科氧化锌丁香酚的疏水性能较强, 其有效成分ZnO可持续释放Zn离子, 呈现出优异的抗菌性能; Dycal和Calcimol具有相似的结构形貌和成分, 但Dycal相对疏水, 能持续释放Ca离子, 在周围形成碱性微环境, 从而赋予其抗菌性和较好的生物相容性; Calcimol为亲水材料, 便于临床操作, 钙离子释放量少, 抗菌性稍弱, 但生物相容性优异。本研究结果为临床上以龋病程度及牙髓健康状况为依据，选择合适的盖髓剂提供了实验基础。

关键词: 盖髓剂, 结构表征, 抗菌性, 生物相容性

Abstract:

Pulp capping agents are effective materials which can preserve dental pulp and treat caries in different ways. It is urgently demanded to establish a guidance to select the appropriate pulp capping agents according to the conditions of pulp and cary requirements. In this work, morphology, composition, physical, and chemical properties of three commonly used clinical pulp capping agents, namely dental zinc oxide eugenol cement (ZnO), self-curing calcium hydroxide (Dycal), and light-curing calcium hydroxide (Calcimol), were studied. Their antibacterial, cytocompatibility and blood compatibility were evaluated. The results showed that ZnO was hydrophobic and its effective component, crystallized ZnO, could consistently release zinc ions, giving its alkaline environment to inhibit bacteria. Structure, morphology and components in Dycal were similar to those in Calcimol. However, its surface was more hydrophobic and its release amount of calcium ions was larger than that of Calcimol. It formed an alkaline micro-environment, thereby possessed good antibacterial ability and biocompatibility. Meanwhile, Calcimol was hydrophilic and convenient to operate, and released less metal ions. Due to its safe composition, Calciomol exhibited excellent biocompatibility but slightly weaker antibacterial property. Our results suggested that these comparative results might be a useful clinical guidance for selecting appropriate pulp capping agents according to the degree of caries and the health status of dental pulp to treat the caries.

Key words: pulp capping agent, microstructure characterization, antibacterial property, biocompatibility

中图分类号:

TQ174

谢家晔, 李力文, 朱强. 三种临床盖髓剂的抗菌性及生物相容性对比研究[J]. 无机材料学报, 2023, 38(12): 1449-1456.

XIE Jiaye, LI Liwen, ZHU Qiang. Contrastive Study on in Vitro Antibacterial Property and Biocompatibility of Three Clinical Pulp Capping Agents[J]. Journal of Inorganic Materials, 2023, 38(12): 1449-1456.

图/表 8

表1 三种材料的组成成分

Table 1 Content of the three samples

Sample		Components
ZnO		Zinc oxide powder Eugenol solution
Dycal	Paste A (13 g)	1,3-butanediol bis(salicylic acid) vinegar Zinc oxide Calcium phosphate Calcium tungstate Iron oxide pigment
Dycal	Paste B (11 g, with catalyst)	Calcium hydroxide N-ethyl-o/p-toluenesulfonamide Zinc oxide Iron dioxide Zinc stearate Iron oxide pigment
Calcimol		Urethane dimethacrylate Barium aluminosilicate Barium sulfonate; Silica Triethylene glycol dimethacrylate Calcium hydroxide

图1 样品表面形貌的SEM照片(a)及其EDS能谱分析结果(b)

Fig. 1 SEM images (a) and EDS results (b) of three samples

图2 样品(a) ZnO、(b) Dycal和(c) Calcimol的XRD图谱

Fig. 2 XRD patterns of (a) ZnO, (b) Dycal and (c) Calcimol

图3 各组样品的表面接触角(a)和表面溶液的pH(b)

Fig. 3 (a) Surface contact angles and (b) pH of surface solution of the samples

图4 ZnO、Dycal和Calcimol样品在超纯水中(a)Ca和(b)Zn离子释放浓度随时间变化

Fig. 4 Immersion time dependence of (a) Ca and (b) Zn ion amounts in pure water containing ZnO, Dycal and Calcimol

图5 大肠杆菌和金黄色葡萄球菌在样品表面培养后的抗菌性能

Fig. 5 Antibacterial ability of all samples on E.coli and S.aureus (a) Cell viability; (b) Photos of bacterial colony; (c) Photos of antibacterial ring

表2 三种盖髓剂理化性能及生物学性能比较

Table 2 Comparisons on physicochemical and biological properties of clinical three common pulp capping agents

Property	ZnO	Dycal	Calcimol
Morphology	Smooth surface	Smooth surface	Porous
Element	C, O, Zn	C, O, Ca, Zn	C, O, Ca
Water contact angle	90°	75°	22°
pH	6.66	9.16	7.90
Antibacteria effect/%	92.6	84.5	82.7
HDPSCs activity/%	6.82	44.54	137.87
Hemolysis ratio/%	8.14	0.99	1.42

图6 ZnO, Dycal和Calcimol samples样品的生物相容性

Fig. 6 Biocompatibility of ZnO, Dycal and Calcimol samples (a) HDPSCs viability; (b) Hemolysis ratio

参考文献 16

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三种临床盖髓剂的抗菌性及生物相容性对比研究

Contrastive Study on in Vitro Antibacterial Property and Biocompatibility of Three Clinical Pulp Capping Agents

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