无机材料学报 ›› 2022, Vol. 37 ›› Issue (4): 436-444.DOI: 10.15541/jim20210158
唐洁吟1,2(), 王刚3,4, 刘聪1,2, 邹学农3,4, 陈晓峰1,2(
)
收稿日期:
2021-03-12
修回日期:
2021-04-08
出版日期:
2022-04-20
网络出版日期:
2021-04-30
通讯作者:
陈晓峰, 教授. E-mail: chenxf@scut.edu.cn作者简介:
唐洁吟(1989-), 女, 硕士. E-mail: 1720175343@qq.com
基金资助:
TANG Jieyin1,2(), WANG Gang3,4, LIU Cong1,2, ZOU Xuenong3,4, CHEN Xiaofeng1,2(
)
Received:
2021-03-12
Revised:
2021-04-08
Published:
2022-04-20
Online:
2021-04-30
Contact:
CHEN Xiaofeng, professor. E-mail: chenxf@scut.edu.cnAbout author:
TANG Jieyin (1989-), female, Master. E-mail: 1720175343@qq.com
Supported by:
摘要:
封闭牙本质小管能有效减轻牙齿过敏症。本研究以不同粒径的微纳米生物活性玻璃球(MNBGs)为分散质、海藻酸钠-磷酸盐缓冲溶液为分散液, 制备了用于牙本质脱敏治疗的MNBGs糊剂(MNBGP)。在牙本质切片表面进行体外矿化并系统评价了糊剂与牙本质的结合性能, 以及糊剂体外诱导牙本质再矿化、封闭牙本质小管的能力。研究结果表明, 不同粒径MNBGs制备的糊剂均能与牙本质界面紧密结合, 粒径较小的MNBGs在脱矿牙本质切片表面分布更加均匀。MNBGP在人工唾液(AS)中能较好地诱导牙本质再矿化形成磷灰石(HA)以堵塞封闭牙本质小管, 脱矿牙本质切片表面形成的HA 层随矿化时间延长而增厚, 矿化28 d HA层的厚度可达到5~10 μm。MNBGs的尺寸影响其诱导牙本质再矿化的效果, 当颗粒大小与牙本质小管直径匹配时, MNBGs可以更好地封闭牙本质小管。因此, MNBGP具有良好的治疗牙本质过敏的应用前景。
中图分类号:
唐洁吟, 王刚, 刘聪, 邹学农, 陈晓峰. 微纳米生物活性玻璃诱导牙本质再矿化研究[J]. 无机材料学报, 2022, 37(4): 436-444.
TANG Jieyin, WANG Gang, LIU Cong, ZOU Xuenong, CHEN Xiaofeng. Dentin Remineralization Induced by Micro-nano Bioactive Glass Spheres[J]. Journal of Inorganic Materials, 2022, 37(4): 436-444.
Sample | SiO2 : CaO : P2O5 (molar ratio) | DDA/g | TEOS/mL | |
---|---|---|---|---|
Theoretical | Measured | |||
MNBGs-1 | 80 : 16 : 4 | 87.637 : 12.363 : 0 | 3 | 8 |
MNBGs-2 | 86.289 : 13.696 : 0.015 | 6 | 16 | |
MNBGs-3 | 89.071 : 10.875 : 0.054 | 6 | 24 |
表1 不同粒径MNBGs的理论、实际化学组分和试剂用量
Table 1 Theoretical and measured chemical composition, reagent dosage of MNBGs with different particle sizes
Sample | SiO2 : CaO : P2O5 (molar ratio) | DDA/g | TEOS/mL | |
---|---|---|---|---|
Theoretical | Measured | |||
MNBGs-1 | 80 : 16 : 4 | 87.637 : 12.363 : 0 | 3 | 8 |
MNBGs-2 | 86.289 : 13.696 : 0.015 | 6 | 16 | |
MNBGs-3 | 89.071 : 10.875 : 0.054 | 6 | 24 |
Samples | C/% | O/% | Ca/% | P/% | Ca/P |
---|---|---|---|---|---|
Without etching | 0 | 65.77 | 20.26 | 13.97 | 1.45 |
With etching | 66.82 | 32.99 | 0.05 | 0.14 | 0.35 |
表2 牙本质切片表面EDTA酸蚀前后各元素含量(摩尔分数)及钙磷比
Table 2 Chemical components (molar percent) and Ca/P ratio on the surface of dentin before and after EDTA etching
Samples | C/% | O/% | Ca/% | P/% | Ca/P |
---|---|---|---|---|---|
Without etching | 0 | 65.77 | 20.26 | 13.97 | 1.45 |
With etching | 66.82 | 32.99 | 0.05 | 0.14 | 0.35 |
图3 牙本质切片样品酸蚀前(a)后(b)的SEM照片(插图为牙本质小管放大照片)及生物活性玻璃糊剂实物照片(c)
Fig. 3 SEM images of the dentin surface without (a) and with (b) EDTA-etching, magnified photo of dentin tubules (insert in (b)), and photo of bioactive glass paste (c)
图5 脱矿牙本质切片表面涂覆不同MNBGP前后, 以及去离子水冲洗后的全反射-红外光谱
Fig. 5 ATR-FTIR spectra of demineralized dentin surface before and after coating with MNBGP, and after rinsing with water (a) MNBGP-1; (b) MNBGP-2; (c) MNBGP-3
图6 未经材料处理(对照组)(a)和涂覆MNBGP-1(b)、MNBGP-2(c)、MNBGP-3(d)的脱矿牙本质切片在人工唾液中浸泡1 d (a1~d1)、7 d (a2~d2)、14 d (a3~d3)和28 d (a4~d4)后的表面SEM照片
Fig. 6 SEM images of the surfaces of demineralized dentin slices without (a) (control) and with treatment by MNBGP-1 (b), MNBGP-2 (c), and MNBGP-3 (d) after soaking in AS for 1 d (a1-d1), 7 d (a2-d2), 14 d (a3-d3) and 28 d (a4-d4)
图7 未经材料处理(a)和涂覆MNBGP-1(b)、MNBGP-2(c)、MNBGP-3(d)的脱矿牙本质切片在人工唾液中浸泡1 d (a1~d1)、7 d (a2~d2)、14 d (a3~d3)和28 d (a4~d4)后的纵截面的SEM照片
Fig. 7 SEM images of the longitudinal section of demineralized dentin samples without (a) (control) and with treatment by MNBGP-1 (b), MNBGP-2 (c), and MNBGP-3 (d) after soaking in AS for 1 d (a1-d1), 7 d (a2-d2), 14 d (a3-d3) and 28 d (a4-d4)
图8 对照组(a)和MNBGP-1(b)、MNBGP-2(c)、MNBGP-3(d)涂覆的脱矿牙本质切片在人工唾液中矿化28 d表面的EDS能谱分析
Fig. 8 EDS analyses of the surface of demineralized dentin slices without (a) (control) and with treatment by MNBGP-1 (b), MNBGP-2 (c), and MNBGP-3 (d) after soaking in AS for 28 d
Sample | Ca/% | P/% | Ca/P |
---|---|---|---|
Control | 2.49 | 1.82 | 1.37 |
MNBGP-1 | 16.64 | 11.69 | 1.42 |
MNBGP-2 | 18.37 | 12.97 | 1.42 |
MNBGP-3 | 6.16 | 6.12 | 1.01 |
表3 脱矿牙本质切片表面钙、磷元素含量(摩尔分数)及钙磷比
Table 3 Chemical components ( molar percent) and Ca/P ratio in molar on the surface of remineralized dentin
Sample | Ca/% | P/% | Ca/P |
---|---|---|---|
Control | 2.49 | 1.82 | 1.37 |
MNBGP-1 | 16.64 | 11.69 | 1.42 |
MNBGP-2 | 18.37 | 12.97 | 1.42 |
MNBGP-3 | 6.16 | 6.12 | 1.01 |
图9 天然牙本质、脱矿牙本质和在人工唾液中浸泡28 d的脱矿牙本质切片样品表面的XRD 图谱
Fig. 9 XRD patterns of the surface of intact dentin, demineralized dentin and slices without treatment (control) and being treated with MNBGP after being soaked in AS for 28 d
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