Li2Ca2Si2O7生物陶瓷的矿化活性研究

doi:10.15541/jim20200657

无机材料学报 ›› 2021, Vol. 36 ›› Issue (7): 753-760.DOI: 10.15541/jim20200657

所属专题：【生物材料】骨骼与齿类组织修复

Li₂Ca₂Si₂O₇生物陶瓷的矿化活性研究

许宏一¹(), 翟东², 曹琬婷¹, 陈振华³, 钱文昊¹(), 陈蕾²()

1.上海市徐汇区牙病防治所, 上海200031
2.中国科学院上海硅酸盐研究所高性能陶瓷和超微结构国家重点实验室, 上海200050
3.烟台正海生物科技股份有限公司, 烟台264006

收稿日期:2020-11-18 修回日期:2020-12-22 出版日期:2021-07-20 网络出版日期:2020-12-30
通讯作者: 钱文昊, 主任医师. E-mail:pingyanlaoto@163.com;陈蕾, 高级工程师. E-mail:chenlei@mail.sic.ac.cn
作者简介:许宏一(1988-), 男, 主管技师. E-mail:13585840803@139.com
基金资助:
国家重点研发计划(2018YFB1105501);上海市自然基金(19ZR1464800);上海市医学领军人才项目(2019LJ27);上海市医学重点专科(ZK2019B12)

Mineralization Activity of Li₂Ca₂Si₂O₇ Bioceramics

XU Hongyi¹(), ZHAI Dong², CAO Wanting¹, CHEN Zhenhua³, QIAN Wenhao¹(), CHEN Lei²()

1. Shanghai Xuhui District Dental Center, Shanghai 200031, China
2. State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China
3. Yantai Zhenghai Bio-tech Co., Ltd, Yantai 264006, China

Received:2020-11-18 Revised:2020-12-22 Published:2021-07-20 Online:2020-12-30
Contact: QIAN Wenhao, chief physician. E-mail:pingyanlaoto@163.com;CHEN Lei, senior engineer. E-mail:chenlei@mail.sic.ac.cn
About author:XU Hongyi(1988-), male, technologist-in-charge. E-mail:13585840803@139.com
Supported by:
National Key Research and Development Plan of China(2018YFB1105501);Shanghai Natural Science Foundation(19ZR1464800);Program for Outstanding Medical Academic Leader(2019LJ27);Shanghai Medical Key Speciaty(ZK2019B12)

摘要/Abstract

摘要：

如何有效治疗牙周炎并实现受损牙周骨组织再生, 一直是牙周疾病治疗中具有挑战性的问题, 而矿化是牙周正常发育和功能中关键因素之一。本研究旨在探讨硅酸钙锂(Li₂Ca₂Si₂O₇)生物陶瓷对人牙周膜成纤维细胞增殖、矿化的影响及用于牙周骨组织再生的可能性。采用溶胶-凝胶法制备合成了Li₂Ca₂Si₂O₇陶瓷粉体。通过体外模拟体液浸泡, 发现Li₂Ca₂Si₂O₇粉体具有良好的羟基磷灰石矿化能力。生物学结果表明: Li₂Ca₂Si₂O₇粉体的浸提液在3.125~25 mg/mL浓度范围内能显著促进HPLFs的增殖, 低浓度(6.25 mg/mL)时可显著诱导HPLFs细胞体外矿化(p<0.05)。Li₂Ca₂Si₂O₇粉体具有促进人牙周膜成纤维细胞增殖和矿化能力, 有望作为牙周骨组织再生修复的生物活性材料。

关键词: Li₂Ca₂Si₂O₇生物陶瓷, 人牙周膜成纤维细胞, 增殖, 矿化

Abstract:

How to effectively treat periodontitis and achieve regeneration of damaged periodontal tissue has always been a challenging problem in the treatment of periodontal diseases, and mineralization is one of the key factors in the normal periodontal development and function. The aim of this study was to investigate the effects of Li₂Ca₂Si₂O₇ bioceramics on proliferation and mineralization of human periodontal ligament fibroblasts (HPLFs) and possibility of its application in periodontal tissue regeneration. Li₂Ca₂Si₂O₇ ceramic powder was synthesized by Sol-Gel method. It was found that Li₂Ca₂Si₂O₇ powder had good ability of hydroxyapatite mineralization by immersing in simulated body fluid. The in vitro biological results showed that Li₂Ca₂Si₂O₇powder extract could significantly promote proliferation of HPLFs in the concentration range of 3.125~25 mg/mL, and at low concentration (6.25 mg/mL), could significantly induce mineralization of HPLFs cells (p<0.05). All these experimental results reveal that the new Li₂Ca₂Si₂O₇powder can promote proliferation and mineralization of HPLFs, and it may be a promising bioactive material for periodontal tissue regeneration.

Key words: Li₂Ca₂Si₂O₇bioceramic, human periodontal ligament fibroblast, proliferation, mineralization

中图分类号:

TQ174

许宏一, 翟东, 曹琬婷, 陈振华, 钱文昊, 陈蕾. Li₂Ca₂Si₂O₇生物陶瓷的矿化活性研究[J]. 无机材料学报, 2021, 36(7): 753-760.

XU Hongyi, ZHAI Dong, CAO Wanting, CHEN Zhenhua, QIAN Wenhao, CHEN Lei. Mineralization Activity of Li₂Ca₂Si₂O₇ Bioceramics[J]. Journal of Inorganic Materials, 2021, 36(7): 753-760.

图/表 8

图1 Li2Ca2Si2O7粉体XRD图谱(A)、SEM形貌(B)和粒径分布(C)

Fig. 1 XRD pattern (A), SEM morphology (B) and particle size distribution (C) of Li2Ca2Si2O7 powders

图2 Li2Ca2Si2O7粉体浸泡SBF不同时间后XRD图谱

Fig. 2 XRD patterns of Li2Ca2Si2O7 powders soaked in SBF for different periods

图3 Li2Ca2Si2O7粉体浸泡SBF不同时间后的SEM照片

Fig. 3 SEM images of Li2Ca2Si2O7 powders soaked in SBF for different periods (A) 0 d; (B) 1 d; (C) 3 d; (D) 7 d; (E) 14 d; (F) 14 d (enlarge image)

图4 Li2Ca2Si2O7粉体浸泡SBF不同时间的各离子浓度

Fig. 4 Ions concentrations of Li2Ca2Si2O7 powders immersed in SBF for different periods

图5 Li2Ca2Si2O7粉体浸提液诱导HPLFs细胞增殖

Fig. 5 Proliferation of HPLFs induced by Li2Ca2Si2O7 powders extract (* p<0.05, ** p<0.01, *** p<0.001)

表1 Li2Ca2Si2O7粉体浸提液的离子浓度

Table 1 Ion concentrations of Li2Ca2Si2O7 powders extracts

Ionic concentration/(mg·L^-1)	Powder extract concentrations /(mg·mL^-1)
Ionic concentration/(mg·L^-1)	Control	3.125	6.25	12.5	25	50	100	200
Li	0	13.45	27.31	53.42	114.31	225.79	440.40	864.14
Ca	69.46	68.61	68.91	65.46	60.36	56.82	46.04	23.16
Si	1.19	13.62	26.16	51.57	102.70	205.01	416.02	840.13
P	28.37	27.48	26.44	25.28	22.19	19.66	13.94	0.71

图6 Li2Ca2Si2O7粉体浸提液诱导HPLFs细胞骨架染色照片

Fig. 6 Cytoskeleton staining of HPLFs induced by Li2Ca2Si2O7 powders extract (A-C) Blank control; (D-F) 6.25 mg/mL; (G-I) 50 mg/mL; Blue: Nucleus; Red: Cytoskeleton

图7 Li2Ca2Si2O7粉体浸提液诱导HPLFs细胞茜素红染色图

Fig. 7 Alizarin red staining of HPLFs induced by Li2Ca2Si2O7 powders extract (A) Blank control; (B) 6.25 mg/mL; (C) 50 mg/mL; (D) Mineralization. *: p<0.05; ***: p<0.001

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Li₂Ca₂Si₂O₇生物陶瓷的矿化活性研究

Mineralization Activity of Li₂Ca₂Si₂O₇ Bioceramics

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