无机材料学报 ›› 2021, Vol. 36 ›› Issue (7): 753-760.DOI: 10.15541/jim20200657
所属专题: 【生物材料】骨骼与齿类组织修复
许宏一1(), 翟东2, 曹琬婷1, 陈振华3, 钱文昊1(
), 陈蕾2(
)
收稿日期:
2020-11-18
修回日期:
2020-12-22
出版日期:
2021-07-20
网络出版日期:
2020-12-30
通讯作者:
钱文昊, 主任医师. E-mail:pingyanlaoto@163.com;陈蕾, 高级工程师. E-mail:chenlei@mail.sic.ac.cn
作者简介:
许宏一(1988-), 男, 主管技师. E-mail:13585840803@139.com
基金资助:
XU Hongyi1(), ZHAI Dong2, CAO Wanting1, CHEN Zhenhua3, QIAN Wenhao1(
), CHEN Lei2(
)
Received:
2020-11-18
Revised:
2020-12-22
Published:
2021-07-20
Online:
2020-12-30
Contact:
QIAN Wenhao, chief physician. E-mail:pingyanlaoto@163.com;CHEN Lei, senior engineer. E-mail:chenlei@mail.sic.ac.cn
About author:
XU Hongyi(1988-), male, technologist-in-charge. E-mail:13585840803@139.com
Supported by:
摘要:
如何有效治疗牙周炎并实现受损牙周骨组织再生, 一直是牙周疾病治疗中具有挑战性的问题, 而矿化是牙周正常发育和功能中关键因素之一。本研究旨在探讨硅酸钙锂(Li2Ca2Si2O7)生物陶瓷对人牙周膜成纤维细胞增殖、矿化的影响及用于牙周骨组织再生的可能性。采用溶胶-凝胶法制备合成了Li2Ca2Si2O7陶瓷粉体。通过体外模拟体液浸泡, 发现Li2Ca2Si2O7粉体具有良好的羟基磷灰石矿化能力。生物学结果表明: Li2Ca2Si2O7粉体的浸提液在3.125~25 mg/mL浓度范围内能显著促进HPLFs的增殖, 低浓度(6.25 mg/mL)时可显著诱导HPLFs细胞体外矿化(p<0.05)。Li2Ca2Si2O7粉体具有促进人牙周膜成纤维细胞增殖和矿化能力, 有望作为牙周骨组织再生修复的生物活性材料。
中图分类号:
许宏一, 翟东, 曹琬婷, 陈振华, 钱文昊, 陈蕾. Li2Ca2Si2O7生物陶瓷的矿化活性研究[J]. 无机材料学报, 2021, 36(7): 753-760.
XU Hongyi, ZHAI Dong, CAO Wanting, CHEN Zhenhua, QIAN Wenhao, CHEN Lei. Mineralization Activity of Li2Ca2Si2O7 Bioceramics[J]. Journal of Inorganic Materials, 2021, 36(7): 753-760.
图3 Li2Ca2Si2O7粉体浸泡SBF不同时间后的SEM照片
Fig. 3 SEM images of Li2Ca2Si2O7 powders soaked in SBF for different periods (A) 0 d; (B) 1 d; (C) 3 d; (D) 7 d; (E) 14 d; (F) 14 d (enlarge image)
Ionic concentration/(mg·L-1) | Powder extract concentrations /(mg·mL-1) | |||||||
---|---|---|---|---|---|---|---|---|
Control | 3.125 | 6.25 | 12.5 | 25 | 50 | 100 | 200 | |
Li | 0 | 13.45 | 27.31 | 53.42 | 114.31 | 225.79 | 440.40 | 864.14 |
Ca | 69.46 | 68.61 | 68.91 | 65.46 | 60.36 | 56.82 | 46.04 | 23.16 |
Si | 1.19 | 13.62 | 26.16 | 51.57 | 102.70 | 205.01 | 416.02 | 840.13 |
P | 28.37 | 27.48 | 26.44 | 25.28 | 22.19 | 19.66 | 13.94 | 0.71 |
表1 Li2Ca2Si2O7粉体浸提液的离子浓度
Table 1 Ion concentrations of Li2Ca2Si2O7 powders extracts
Ionic concentration/(mg·L-1) | Powder extract concentrations /(mg·mL-1) | |||||||
---|---|---|---|---|---|---|---|---|
Control | 3.125 | 6.25 | 12.5 | 25 | 50 | 100 | 200 | |
Li | 0 | 13.45 | 27.31 | 53.42 | 114.31 | 225.79 | 440.40 | 864.14 |
Ca | 69.46 | 68.61 | 68.91 | 65.46 | 60.36 | 56.82 | 46.04 | 23.16 |
Si | 1.19 | 13.62 | 26.16 | 51.57 | 102.70 | 205.01 | 416.02 | 840.13 |
P | 28.37 | 27.48 | 26.44 | 25.28 | 22.19 | 19.66 | 13.94 | 0.71 |
图6 Li2Ca2Si2O7粉体浸提液诱导HPLFs细胞骨架染色照片
Fig. 6 Cytoskeleton staining of HPLFs induced by Li2Ca2Si2O7 powders extract (A-C) Blank control; (D-F) 6.25 mg/mL; (G-I) 50 mg/mL; Blue: Nucleus; Red: Cytoskeleton
图7 Li2Ca2Si2O7粉体浸提液诱导HPLFs细胞茜素红染色图
Fig. 7 Alizarin red staining of HPLFs induced by Li2Ca2Si2O7 powders extract (A) Blank control; (B) 6.25 mg/mL; (C) 50 mg/mL; (D) Mineralization. *: p<0.05; ***: p<0.001
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