可注射磁性液固相变材料用于骨肉瘤的磁共振成像与低温磁热治疗研究

doi:10.15541/jim20200016

无机材料学报 ›› 2020, Vol. 35 ›› Issue (11): 1277-1282.DOI: 10.15541/jim20200016

所属专题：生物材料论文精选(2020)；【虚拟专辑】生物检测与成像(2020~2021)

可注射磁性液固相变材料用于骨肉瘤的磁共振成像与低温磁热治疗研究

徐东^{1, 2}, 朱钰方^{1, 2}, 郑元义³, 罗宇^{2, 4}, 陈航榕²

1. 上海理工大学材料科学与工程学院, 上海 200093;
2. 中国科学院上海硅酸盐研究所, 上海 200050;
3. 上海交通大学附属第六人民医院上海超声医学研究所, 上海 200233;
4. 同济大学化学科学与工程学院, 上海 200092

收稿日期:2020-01-09 修回日期:2020-01-21 出版日期:2020-11-20 网络出版日期:2020-05-20
作者简介:徐东(1995-), 男, 硕士研究生. E-mail: xudongelio@gmail.com.
基金资助:
国家自然科学基金国际合作重点项目(81720108023); 上海市基础研究重点项目(19JC1415600); Key Project of International Cooperation and Exchange of NSFC (81720108023); Key Program for Basic Research of Shanghai (19JC1415600)

Injectable Magnetic Liquid-solid Phase Transition Material for MR Imaging and Low-temperature Magnetocaloric Therapy of Osteosarcoma

XU Dong^{1, 2}, ZHU Yufang^{1, 2}, ZHENG Yuanyi³, LUO Yu^{2, 4}, CHEN Hangrong²

1. School of Materials Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;
2. Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China;
3. Shanghai Institute of Ultrasound in Medicine, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China;
4. School of Chemical Science and Engineering, Tongji University, Shanghai 200092, China

Received:2020-01-09 Revised:2020-01-21 Published:2020-11-20 Online:2020-05-20
About author:XU Dong (1995-), male, Master candidate. E-mail: xudongelio@gmail.com

摘要/Abstract

摘要： 过高温可诱发肿瘤周围正常组织产生炎症以及热辐射损伤。因此研发一种能在相对较低温度下(例如43 ℃)即可实现肿瘤细胞高致死率的磁性材料对于磁热治疗的临床应用至关重要。本研究聚焦低温、安全、高效磁热疗, 选取FDA批准的液固相变材料聚乳酸-羟基乙酸(PLGA)为原料, 装载一步温和还原法制备得到的超顺磁性氧化铁纳米颗粒, 用于磁共振成像和磁热升温; 进一步在PLGA中装载热休克蛋白HSP90的小分子抑制剂-表没食子儿茶素没食子酸酯(EGCG), 抑制机体受热保护功能, 实现较低温度下杀死肿瘤细胞。体外实验结果表明, 制备得到的超顺磁性氧化铁纳米颗粒不仅拥有良好的T₂加权成像性能, 还具有优良的磁热升温性能。所制备的PLGA/Fe₃O₄/EGCG复合材料在交变磁场下控制升温至43 ℃并保温40 min后发现肿瘤细胞死亡率达70%, 显示出针对骨肉瘤低温磁热治疗的良好潜力。这种可注射磁热相变材料将为骨肉瘤的治疗提供新的思路和材料支撑。

关键词: 可注射, 液固相变, 低温磁热, 磁共振成像, 骨肉瘤

Abstract: Overhigh temperature can induce inflammation and heat radiation damage to normal tissues around the tumor. Therefore, the development of a magnetic material that can achieve high mortality of tumor cells at relatively low temperatures (e.g. 43 ℃) is critical for the clinical application of magnetocaloric therapy. This study focuses on the goal of low-temperature, safe, and effective magnetic thermotherapy. The liquid-solid phase transition material polylactic acid-glycolic acid (PLGA) approved by the FDA, is selected as raw material and superparamagnetic iron oxide nanoparticles prepared by a one-step mild reduction method are loaded to achieve magnetic resonance imaging and magnetic heating. Meanwhile, a small molecule inhibitor of heat shock protein HSP90, i.e, epigallocatechin gallate (EGCG), encapsulated in PLGA, could inhibit the body’s thermal protection function and achieve the purpose of killing tumor cells at lower temperatures. In vitro results indicated that the prepared superparamagnetic iron oxide nanoparticles not only have good T₂-weighted imaging performance, but also show excellent magnetothermal transformation performance. More details, when the obtained PLGA/Fe₃O₄/EGCG biocomposite was controlled to heated up to 43 ℃ for 40 min under an alternating magnetic field, around 70% of tumor cell could be killed, exhibiting promising potential for low-temperature magnetothermal treatment of tumor. Such novel injectable magnetic thermal phase transition material may provide new idea and material support for the treatment of osteosarcoma.

Key words: injectable, liquid-solid phase transition, low temperature magnetocaloric therapy, magnetic resonance imaging, osteosarcoma

中图分类号:

TQ174

徐东, 朱钰方, 郑元义, 罗宇, 陈航榕. 可注射磁性液固相变材料用于骨肉瘤的磁共振成像与低温磁热治疗研究[J]. 无机材料学报, 2020, 35(11): 1277-1282.

XU Dong, ZHU Yufang, ZHENG Yuanyi, LUO Yu, CHEN Hangrong. Injectable Magnetic Liquid-solid Phase Transition Material for MR Imaging and Low-temperature Magnetocaloric Therapy of Osteosarcoma[J]. Journal of Inorganic Materials, 2020, 35(11): 1277-1282.

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可注射磁性液固相变材料用于骨肉瘤的磁共振成像与低温磁热治疗研究

Injectable Magnetic Liquid-solid Phase Transition Material for MR Imaging and Low-temperature Magnetocaloric Therapy of Osteosarcoma

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