钛网表面含hBMP-2的复合涂层制备及hBMP-2的释放研究

doi:10.15541/jim20190127

无机材料学报 ›› 2020, Vol. 35 ›› Issue (2): 173-178.DOI: 10.15541/jim20190127

所属专题：生物材料论文精选(2020)；【虚拟专辑】药物递送(2020~2021)

钛网表面含hBMP-2的复合涂层制备及hBMP-2的释放研究

付亚康¹,翁杰²,刘耀文³,张科宏¹

1. 雅安职业技术学院, 雅安 625000
2. 西南交通大学材料科学与工程学院, 材料先进技术教育部重点实验室, 成都 610031
3. 四川农业大学食品学院, 雅安 625014

收稿日期:2019-03-25 修回日期:2019-05-04 出版日期:2020-02-20 网络出版日期:2019-05-29
作者简介:付亚康(1988-), 男, 硕士研究生, 讲师. E-mail: fykmail@163.com
基金资助:
国家自然科学基金(51572228);四川省教育厅自然科学基金(16ZB0546);雅安职业技术学院自然科学基金(2016yzk15)

hBMP-2 Contained Composite Coatings on Titanium Mesh Surface: Preparation and hBMP-2 Release

FU Ya-Kang¹,WENG Jie²,LIU Yao-Wen³,ZHANG Ke-Hong¹

1. Ya’an Polytechnic College, Ya’an 625000, China
2. Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China
3. School of food, Sichuan Agricultural University, Ya’an 625014, China

Received:2019-03-25 Revised:2019-05-04 Published:2020-02-20 Online:2019-05-29
Supported by:
National Natural Science Foundation of China(51572228);Natural Science Foundation Project of Sichuan Provincial Education Department(16ZB0546);Natural Science Foundation Project of Ya' an Polytechnic College(2016yzk15)

摘要/Abstract

摘要：

为提高骨接合钛网的骨整合性和生物活性, 本研究采用碱热处理法在钛网表面构建出具有多孔结构的钛酸盐纳米纤维, 利用电化学沉积技术在钛酸盐纳米纤维表面制备磷酸钙涂层, 并采用不同方法将人骨形态发生蛋白(hBMP-2)引入涂层, 制备了三种含hBMP-2分子的复合涂层(TmhB、TmHedhB和TmHhBed)。实验对各复合涂层的表面形貌、化学成分、相组成和hBMP-2的含量与释放性能进行了表征。研究发现: 各涂层都具有多孔纤维结构, TmHedhB和TmHhBed中的磷酸钙相为羟基磷灰石(HA), 呈“串珠”状包裹在钛酸盐纳米纤维表面, “串珠”状HA的引入促进了复合涂层对hBMP-2的吸附。电化学共沉积技术在钛酸盐纳米纤维表面制备的HA/hBMP-2复合涂层中hBMP-2的含量最大, 达886 ng/mg, 在6~48 h内具有明显的hBMP-2缓释性能。

关键词: 钛, 碱热处理, 电化学沉积, 羟基磷灰石, 复合涂层, hBMP-2

Abstract:

Titanate nanofibers were prepared on titanium mesh by alkali-heat treatment, and calcium phosphate coating was fabricated on the porous titanate nanofibers by electrochemical deposition technology. Then hBMP-2 was introduced into the coating by different methods to improve its osteointegration and bioactivity. Three kinds of composite coatings modified by hBMP-2 were prepared (TmhB, TmHedhB and TmHhBed). Surface morphology, chemical composition, phase composition and hBMP-2 amounts and hBMP-2 release performance of the composite coatings were characterized by SEM, ATR-FTIR, XRD, and hBMP-2 ELISA kit, respectively. Results showed that all of the coatings display porous fiber structure, calcium phosphate phase in TmHedhB and TmHhBed samples was hydroxyapatite (HA), and bead-like HA particles formed on the surface of titanate nanofibers. Protein adsorption experiments showed that introduction of bead-like HA phase increased the hBMP-2 adsorption on the composite coatings, and composite coatings prepared by electrochemical co-deposition technique further enhanced hBMP-2 adsorption up to 886 ng/mg, which were supported hBMP-2 sustained release within 6-48 h.

Key words: titanium, alkali-heat treatment, electrochemical deposition, hydroxyapatite, composite coating, hBMP-2

中图分类号:

R318
TQ174

付亚康,翁杰,刘耀文,张科宏. 钛网表面含hBMP-2的复合涂层制备及hBMP-2的释放研究[J]. 无机材料学报, 2020, 35(2): 173-178.

FU Ya-Kang,WENG Jie,LIU Yao-Wen,ZHANG Ke-Hong. hBMP-2 Contained Composite Coatings on Titanium Mesh Surface: Preparation and hBMP-2 Release[J]. Journal of Inorganic Materials, 2020, 35(2): 173-178.

图/表 7

图1 电化学共沉积技术在钛网表面制备复合涂层的示意图

Fig. 1 Schematic diagram of preparation of composite coating on titanium mesh surface by electrochemical co-deposition

表1 钛网经碱热处理后各样品的不同制备方法

Table 1 Special fabrication procedures of titanium mesh after alkali-heat treatment

Sample	Special fabrication procedure
TmhB	hBMP-2 adsorption
TmHedhB	HA electrochemical deposition followed by hBMP-2 adsorption
TmHhBed	hBMP-2/HA electrochemical co-deposition

图2 钛网表面不同复合涂层的ATR-FTIR图谱

Fig. 2 ATR-FTIR spectra of different composite coatings on titanium mesh surface

图3 钛网表面不同复合涂层的XRD图谱

Fig. 3 XRD images of different composite coatings on titanium mesh surface

图4 钛网表面不同复合涂层的SEM照片

Fig. 4 SEM images of different composite coatings on titanium mesh surface (a-c) TmhB; (d-f) TmHedhB; (g-i) TmHhBed

图5 钛网表面不同复合涂层中hBMP-2的含量

Fig. 5 hBMP-2 content in different composite coatings on titanium mesh surface (*P < 0.05)

图6 钛网表面不同复合涂层中hBMP-2的释放曲线

Fig. 6 Release curves of hBMP-2 in different composite coatings on titanium mesh surface

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钛网表面含hBMP-2的复合涂层制备及hBMP-2的释放研究

hBMP-2 Contained Composite Coatings on Titanium Mesh Surface: Preparation and hBMP-2 Release

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