钛表面铜离子注入对细菌和细胞行为的影响

doi:10.15541/jim20190105

无机材料学报 ›› 2020, Vol. 35 ›› Issue (2): 158-164.DOI: 10.15541/jim20190105

所属专题：生物材料论文精选(2020)；【虚拟专辑】抗菌材料(2020~2021)

钛表面铜离子注入对细菌和细胞行为的影响

李昆强^1,²,乔玉琴¹,刘宣勇^1,²()

1. 中国科学院上海硅酸盐研究所, 高性能陶瓷和超微结构国家重点实验室, 上海 200050
2. 中国科学院大学材料科学与光电子工程中心, 北京 100049

收稿日期:2019-03-09 修回日期:2019-04-03 出版日期:2020-02-20 网络出版日期:2019-05-29
作者简介:李昆强(1993-), 男, 硕士研究生. E-mail: kunqiangli@163.com
基金资助:
国家重点研发计划(2016YFC1100604);国家自然科学基金重点项目(51831011);国家重点实验室主任青年基金(SKL201606)

Titanium Modified by Copper Ion Implantation: Anti-bacterial and Cellular Behaviors

LI Kun-Qiang^1,²,QIAO Yu-Qin¹,LIU Xuan-Yong^1,²()

1. The State Key Lab of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China
2. Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049, China

Received:2019-03-09 Revised:2019-04-03 Published:2020-02-20 Online:2019-05-29
Supported by:
National Key Research and Development Program of China(2016YFC1100604);National Natural Science Foundation of China(51831011);Science Foundation for Youth Scholar of State Key Laboratory of High Performance Ceramics and Superfine Microstructures(SKL201606)

摘要/Abstract

摘要：

医用钛及其合金被广泛用作骨组织替换材料, 但缺乏抗菌性, 易导致细菌感染。铜具有良好的抗菌性能, 将其引入到钛表面, 可改善医用钛的抗菌性能; 然而铜含量过高对细胞具有毒性。因此, 需要调节铜的含量, 实现铜的抗菌性能和细胞相容性之间的平衡。本研究采用等离子体浸没离子注入技术对医用钛进行表面改性, 获得表面含铜量不同的样品, 并研究改性钛表面对细菌和细胞行为的影响。结果表明, 钛表面含铜量较低的样品能够促进大鼠骨髓间充质干细胞(rBMSCs)和人脐静脉内皮细胞(HUVECs)的增殖, 但对大肠杆菌和金黄色葡萄球菌没有抑制能力; 随着离子注入时间的延长, 钛表面含铜量较高的样品抗菌能力显著提高, 同时也未产生明显细胞毒性。因此, 通过控制钛表面的铜含量, 可以获得兼具良好抗菌性能和生物相容性的钛植入材料。

关键词: 离子注入, 生物相容性, 铜, 抗菌

Abstract:

Titanium and its alloys have been wildly used as bone implants. However, it is still facing a severer issue: implant related infections due to the lack of antibacterial ability. Copper (Cu) has good antibacterial ability and can be used to improve the anti-infection capability of titanium. In this study, three kinds of Ti samples with different contents of Cu in the modified layer were prepared by plasma immersion ion implantation (PIII) technology, and their responses to bacteria and cells were explored in vitro. The results showed that the sample with low Cu content at the surface could promote the proliferation of rat bone marrow mesenchymal stem cells (rBMSCs) and human umbilical vein endothelial cells (HUVECs) but not inhibit the proliferation of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). As the implantation time extends, antibacterial ability of the samples with high Cu content at the surface was significantly enhanced, and no obvious cytotoxicity was observed. Therefore, it is possible to acquire a balance between antibacterial ability and biocompatibility of Ti by controlling the contents of Cu in the modified layer.

Key words: ion implantation, biocompatibility, copper, antibacterial

中图分类号:

R318

李昆强,乔玉琴,刘宣勇. 钛表面铜离子注入对细菌和细胞行为的影响[J]. 无机材料学报, 2020, 35(2): 158-164.

LI Kun-Qiang,QIAO Yu-Qin,LIU Xuan-Yong. Titanium Modified by Copper Ion Implantation: Anti-bacterial and Cellular Behaviors[J]. Journal of Inorganic Materials, 2020, 35(2): 158-164.

图/表 7

表1 铜等离子体浸没离子注入的主要参数

Table 1 Instrumental parameters of Cu-PⅢ

Parameters	Target	Cathodic arc
Pulsing frequency /Hz	10	10
Voltage pulse duration /μs	500	500
Implantation Voltage /kV	-30	-
Pressure /Pa	5×10^-3	-

图1 不同样品表面在低倍(a~d)和高倍(e~h)镜下的SEM形貌

Fig. 1 Surface SEM morphologies of samples under low (a-d) and high (e-h) magnification

表2 不同样品表面铜的含量

Table 2 Cu contents at the surfaces of various samples explored with XPS

Sample	Cu-1h	Cu-2h	Cu-3h
Cu content/at%	2.54	5.12	5.99

图2 不同样品表面(a, c, e)和30 nm深度处(b, d, f)的Cu 2p的高分辨XPS图谱

Fig. 2 Cu 2p high-resolution XPS spectra at the surface (a, c, e) and the interior at 30 nm depth (b, d, f) of various samples

图3 不同样品表面的水接触角(a), 不同样品在0.9wt% NaCl溶液中测得Tafel曲线(b)和不同样品在PBS中浸泡1、4、7和14 d的铜离子释放曲线(c)

Fig. 3 Water contact angles of samples’ surfaces (a), Tafel curves of samples in 0.9wt% NaCl solution (b), and Cu ions release of samples after immersion in PBS for 1, 4, 7 and 14 d (c)

图4 不同样品表面大肠杆菌(a,c,e)和金黄色葡萄球菌(b,d,f)的SEM照片(a,b)、在琼脂板上培养后的菌落生长状况(c,d)和阿尔玛蓝共培养后菌液的荧光强度(e,f)

Fig. 4 SEM morphologies of E. coli (a,c,e) and S. aureus (b,d,f) cultured on various samples(a,b), bacterial colonies on agar culture plates(c,d) and fluorescence intensities of Alamar blue for bacterias cultured on various samples (e,f)

图5 rBMSCs(a)和HUVECs(b)在不同样品表面培养1、4和7 d后的阿尔玛蓝溶液的荧光强度

Fig. 5 Fluorescence intensities of alamar Blue for rBMSCs (a) and HUVECs (b) cultured on different sample surfaces for 1, 4, and 7 d *p < 0.05, **p < 0.01, ***p < 0.001

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钛表面铜离子注入对细菌和细胞行为的影响

Titanium Modified by Copper Ion Implantation: Anti-bacterial and Cellular Behaviors

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