Ni-Ti-LDHs纳米片对阿司匹林的负载与缓释

doi:10.15541/jim20190074

摘要/Abstract

摘要：

采用插层法和剥离-重组法制备了阿司匹林-双金属氢氧化物复合物(A-LDHs)和阿司匹林-双金属氢氧化物纳米片复合物(A-LDHs-NS)。采用XRD、SEM、TG-DTG和FT-IR对复合物的形貌、载药性能和载药模型进行表征。测定了阿司匹林在不同pH环境中从A-LDHs和A-LDHs-NS上的释放性能。研究发现, 实验制备的LDHs和LDHs-NS均具有明显层状结构。LDHs-NS因具有较大的比表面积(187 m ²·g ^-1)能负载更多的阿司匹林。同时, LDHs-NS与阿司匹林之间有较强的相互作用, 载药量为1.178 mmol·g ^-1, 释放时间超过1440 min, 与对照组(20 min)相比, 表现出更优异的缓释性能, 且在pH为7.4的磷酸盐缓冲溶液中缓释性能比在pH为4.8中更强。本研究结果可以为二维材料在生物医药中的应用提供参考。

关键词: 阿司匹林, 双金属氢氧化物纳米片, 负载, 缓释性能, 相互作用模型

Abstract:

Aspirin/layered double hydroxides composite (A-LDHs) and aspirin/layered double hydroxide-nanosheets composite (A-LDHs-NS) were prepared by intercalation and exfoliation-recombination, respectively. Their morphology, drug loading property and drug loading mode of the composites were characterized by XRD, SEM, TG-DTG, and FT-IR. The releasing performances of aspirin from A-LDHs and A-LDHs-NS in the phosphate buffer solutions with different pH conditions were investigated. The results show that the typical lamellar structures are hold in as-prepared LDHs and LDHs-NS. The larger specific surface area (187 m ²·g ^-1) of LDHs-NS, the more aspirins are loaded on its surface, among which the max drug loading is 1.178 mmol·g ^-1. Its releasing process lasts for more than 1440 min, much longer than 20 min of the control materials, showing excellent sustained-releasing performance. This performance may because of the strong interaction between aspirin and LDHs-NS. Moreover, the sustained releasing performance is stronger at pH 7.4 than that at pH 4.8. All data from this study provides a reference for wider application of this kind of two-dimensional materials in biomedicine.

Key words: aspirin, layered double hydroxides nanosheet, drug loading, sustained-release, interaction mode

中图分类号:

TQ462

胡丽芳,刘柳,何杰,孙志鹏,陈小平. Ni-Ti-LDHs纳米片对阿司匹林的负载与缓释[J]. 无机材料学报, 2020, 35(2): 165-172.

HU Li-Fang,LIU Liu,HE Jie,SUN Zhi-Peng,CHEN Xiao-Ping. Aspirin on Ni-Ti-LDHs Nanosheets: Load and Sustained-release[J]. Journal of Inorganic Materials, 2020, 35(2): 165-172.

图/表 9

图1 样品的XRD图谱

Fig. 1 XRD patterns of as-prepared samples (a) Ni-Ti-LDHs; (b) A-LDHs-1; (c)A-LDHs-2; (d) A-LDHs-5; (e) Ni-Ti-LDHs-NS; (f) A-LDHs-NS-1; (g) A-LDHs-NS-2; (h) A-LDHs-NS-5

图2 制备样品的SEM照片

Fig. 2 SEM images of as-prepared samples (a) Ni-Ti-LDHs; (b) A-LDH-1; (c) Ni-Ti-LDHs-NS; (d)A-LDHs-NS-1

图3 样品的TG-DTG 曲线

Fig. 3 TG-DTG curves of as-prepared samples (a) Ni-Ti-LDHs; (b) A-LDHs-1; (c)A-LDHs-2; (d) A-LDHs-5; (e) Ni-Ti-LDHs-NS; (f) A-LDHs-NS-1; (g) A-LDHs-NS-2; (h) A-LDHs-NS-5

图4 样品的N2吸附-脱附等温线(SBET指比表面积)

Fig. 4 N2 adsorption-desorption isotherms of as-prepared samples (SBET represents the specific surface area) (a) Ni-Ti-LDHs; (b) Ni-Ti-LDHs-NS

图5 A-LDHs与A-LDHs-NS释放阿司匹林后的FT-IR谱图

Fig. 5 FT-IR spectra of A-LDHs and A-LDHs-NS after drug release (a) Ni-Ti-LDHs; (b) A-LDHs-1; (c) A-LDHs-2; (d) A-LDHs-5; (e) Ni-Ti-LDHs-NS; (f) A-LDHs-NS-1; (g) A-LDHs-NS-2; (h) A-LDHs-NS-5

图6 阿司匹林与Ni-Ti-LDHs及Ni-Ti-LDHs-NS可能的复合模型示意图

Fig. 6 Schematic diagram of the composite model of aspirin with Ni-Ti-LDHs and Ni-Ti-LDHs-NS

图7 阿司匹林在pH为4.8和7.4的PBS中的紫外-可见吸收光谱(a, a1)和标准工作曲线(b, b1)

Fig. 7 UV-Vis absorption spectra (a,a1) and standard operating curves (b,b1) of aspirin in PBS with pH=4.8 and 7.4

图8 Ni-Ti-LDHs(a, a1)和Ni-Ti-LDHs-NS(b, b1)在pH为4.8和7.4的PBS中不同时间的紫外-可见吸收光谱

Fig. 8 UV-Vis absorption spectra of Ni-Ti-LDHs (a, a1) and Ni-Ti-LDHs-NS (b,b1) over time in PBS with pH=4.8 and 7.4

图9 阿司匹林在pH为4.8(A1, A2)和7.4(B1.B2)的PBS中从复合材料中的释放曲线

Fig. 9 Release curves of aspirin in PBS with pH=4.8 (A1, A2) and 7.4 (B1, B2) from different composites A-LDHs-1 (a), A-LDHs-2 (b), A-LDHs-5 (c), A-LDHs-NS-1 (d), A-LDHs-NS-2 (e), and A-LDHs-NS-5 (f)

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