微流控技术制备ZnO纳米棒及生物荧光检测性能研究

doi:10.15541/jim20180005

摘要/Abstract

摘要：

本研究设计并制备了一种微流控芯片并在其中水热合成了氧化锌(ZnO)纳米棒。利用扫描电子显微镜(SEM)和X射线衍射(XRD)研究了合成条件对ZnO纳米棒的形貌和晶体结构的影响。结果表明, 在微流控芯片中可制备得到致密的ZnO纳米棒, 其直径和长度随加热方式和制备时间的变化而改变。对比研究不同加热方式制备的ZnO纳米棒阵列检测异硫氰酸荧光素标记的羊抗牛IgG的性能, 发现局部加热方式制备的ZnO纳米棒检测荧光素标记蛋白的性能更佳, 在10 pg/mL~1 μg/mL范围内线性良好, 相关系数为0.99209。在此基础上, 用局部加热制备的ZnO纳米棒检测人甲胎蛋白(AFP), 其最低检测限可达1 pg/mL。这些结果表明, 微通道中合成的ZnO纳米棒适用于多通道荧光检测。

关键词: ZnO, 微流控芯片, 荧光检测, 生物传感器

Abstract:

A simple microfluidic chip was designed and fabricated, on which zinc oxide (ZnO) nanorods were synthesized by hydrothermal method. The influence of growth condition on morphology and crystal structure of ZnO nanorods were investigated by scanning electron microscope (SEM) and X-ray diffraction (XRD), respectively. Experimental results showed that densely packed ZnO nanorods could be prepared on microfluidic chip, with good crystallinity and c-axis orientation. The diameter and length of ZnO nanorods changed with heating methods and growth time. Moreover, fluorescence detection by ZnO nanorods, synthesized with different heating methods, were evaluated by FITC-labeled goat anti-bovine IgG. And the results revealed that ZnO nanorods synthesized on chip by localized heating showed better detection performance of fluorescein-labeled protein and good linearity from 10 pg/mL to 1 μg/mL with the correlation coefficient of 0.99209. The as-synthesized ZnO nanorods were further used to demonstrate detection of human α-fetoprotein (AFP), and achieved a superior limit of detection as low as 1 pg/mL. This work demonstrated that ZnO nanorods synthesized by microfluidic device allowed multiplexed fluorescence biodetection.

Key words: ZnO, microfluidic chip, fluorescence detection, biosensor

中图分类号:

O611

郭灵霞, 施雨辰, 赵振杰, 李欣. 微流控技术制备ZnO纳米棒及生物荧光检测性能研究[J]. 无机材料学报, 2018, 33(10): 1103-1109.

GUO Ling-Xia, SHI Yu-Chen, ZHAO Zhen-Jie, LI Xin. Fabrication and Fluorescence Biodetection of ZnO Nanorods Using Microfluidic Technology[J]. Journal of Inorganic Materials, 2018, 33(10): 1103-1109.

图/表 6

图1 微流控芯片示意图(a); 利用整体加热(b)及局部加热(c)方法在芯片中合成 ZnO 纳米棒的实验照片

Fig. 1 Schematic of microfluidic chip (a), photograph of synthesis of ZnO nanorods on chip by global heating (b) and localized heating (c)

图2 ZnO纳米棒在整体加热生长0.5 (a)、1 (b)和3 h(c)后以及局部加热生长0.5 (d)、1 (e)和3 h(f)的SEM照片

Fig. 2 SEM images of ZnO nanorods grown by global and localized heating method for 0.5 h (a, d), 1 h (b, e) and 3 h(c, f), respectively

图3 整体加热0.5, 1和3 h (a)~(c)、局部加热0.5, 1和3 h (d)~(f)条件下制备的ZnO纳米棒的直径分布以及直径(g)和长度(h)随时间的变化

Fig. 3 Diameter distribution histograms of ZnO nanorods grown for different time by different heating methods and diameter variation (g) and length variation (h) of ZnO nanorods as a function of growth time (a)-(c): 0.5 h, 1 h and 3 h by global heating; (d)-(f): 0.5 h, 1 h and 3 h by localized heating

图4 ZnO纳米棒的XRD图谱

Fig. 4 XRD patterns of ZnO nanorods

图5 整体(a)~(b)加热和局部(c)~(d)加热制备的ZnO纳米棒检测FITC标记的羊抗牛IgG的荧光照片以及荧光强度曲线

Fig. 5 Fluorescence images and corresponding detection curve of FITC-anti bovine IgG on ZnO nanorods synthesized by global heating (a-b) and localized heating (c-d)

图6 局部加热制备的ZnO纳米棒检测AFP的荧光照片(a)和荧光强度随AFP浓度变化曲线(b), 以及不同蛋白(BSA, PSA)干扰下检测AFP的荧光照片(c)和荧光强度的变化(d)

Fig. 6 Fluorescence images (a) and quantitative analysis of fluorescence intensity with various AFP concentration (b) on ZnO nanowires synthesized by localized heating, as well as fluorescence images (c) and quantitative analysis of fluorescence intensity with AFP detection (d) against other potentially interfering proteins using BSA and PSA Various proteins in each group have the same concentration, 1 μg/mL

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