SLA表面PDA/CPP复合涂层的制备及其性能研究

doi:10.15541/jim20170055

无机材料学报 ›› 2017, Vol. 32 ›› Issue (12): 1264-1268.DOI: 10.15541/jim20170055

SLA表面PDA/CPP复合涂层的制备及其性能研究

张嘉敏¹, 汪涛¹, 汤春波², 汪乔那², 钱海梅¹, 缪润杰¹

1. 南京航空航天大学材料科学与技术学院, 南京 211106;
2. 江苏省口腔医院, 南京 210029

收稿日期:2017-01-24 修回日期:2017-03-15 出版日期:2017-12-20 网络出版日期:2017-11-21
作者简介:张嘉敏(1992-), 女, 硕士研究生. E-mail: zjmmandy@163.com
基金资助:
中央高校基本科研业务费(NP2012303);江苏高校优势学科建设工程资助项目;Fundamental Research Funds for the Central Universities (NP2012303);Priority Academic Program Development of Jiangsu Higher Education Institutions

Preparation and Property of PDA/CPP Bilayer on SLA Surfaces

ZHANG Jia-Min¹, WANG Tao¹, TANG Chun-Bo², WANG Qiao-Na², QIAN Hai-Mei¹, MIAO Run-Jie¹

1. College of Materials Science and Technology, Nanjing University of Aeronautics and Astronautics, Nanjing 211106, China;
2. Stomatological Hospital of Jiangsu Province, Nanjing 210029, China

Received:2017-01-24 Revised:2017-03-15 Published:2017-12-20 Online:2017-11-21

摘要/Abstract

摘要：

在大颗粒喷砂酸蚀(SLA)工艺形成的三维多级嵌套孔洞结构纯钛表面, 利用聚多巴胺(PDA)的超强粘附性和二次修饰功能, 接枝生物大分子酪蛋白磷酸肽(CPP), 制备PDA/CPP复合涂层。采用FESEM、EDS、XPS、水接触角分析仪、体外仿生矿化测试以及人骨髓间充质干细胞(hBMSCs)实验对样品表面进行表征分析和性能测试。结果表明, 两步浸泡法成功在SLA表面制得PDA/CPP复合涂层。该涂层在保留SLA多孔形貌的同时, 使水接触角从47.7°减小到25.5°, 明显提高了SLA表面亲水性; 在模拟体液浸泡1 d后的修饰表面即沉积了致密羟基磷灰石(HA), 说明PDA/CPP显著加快了SLA表面生物矿化的速度。同时, SLA/PDA/CPP表面细胞粘附、增殖以及矿化活性都得到了明显改善。

关键词: 聚多巴胺, 酪蛋白磷酸肽, 喷砂酸蚀, 纯钛, 生物活化

Abstract:

A polydopamine/casein phosphopeptides (PDA/CPP) bilayer was prepared on the pure titanium surface with macro and micro pits formed by sandblasting with large-grit and acid-etching (SLA). The PDA interlayer with extraordinary adhesion and the capability of second functional modification, linked CPP, the natural biomolecules, to the SLA titanium surface via covalent bonds. This two-step immersion modification process was simple and feasible. Field emission scanning electronic microscope (FESEM), energy dispersive spectroscope (EDS) and X-ray photoelectron spectroscppe (XPS) were utilized to analyze microtopography and elementary composition of PDA/CPP bilayer. Contact angle analysis, biomimetic mineralization examinations and human bone marrow stem cells (hBMSCs) experiments in vitro were conducted to verify the biocompatibility of the modified surfaces. The results showed that the PDA/CPP bilayer was successfully grafted onto the SLA substrates through the two-step immersion method. The bilayer decreased the contact angle of SLA with pure water from 47.7° to 25.5°, showing the improved hydrophilicity obviously. Compact hydroxyapatite (HA) layer was formed after SLA/PDA/CPP immersed in simulated body fluid (SBF) for 1 d, demonstrating that the bilayer accelerated the rate of biomineralization significantly. Besides, the PDA/CPP bilayer efficiently enhanced the cell attachment, cell proliferation and cell mineralization.

Key words: polydopamine, casein phosphopeptides, SLA, pure titanium, bio-activation

中图分类号:

TQ178

张嘉敏, 汪涛, 汤春波, 汪乔那, 钱海梅, 缪润杰. SLA表面PDA/CPP复合涂层的制备及其性能研究[J]. 无机材料学报, 2017, 32(12): 1264-1268.

ZHANG Jia-Min, WANG Tao, TANG Chun-Bo, WANG Qiao-Na, QIAN Hai-Mei, MIAO Run-Jie. Preparation and Property of PDA/CPP Bilayer on SLA Surfaces[J]. Journal of Inorganic Materials, 2017, 32(12): 1264-1268.

图/表 5

图1 改性前后SLA表面FESEM照片

Fig. 1 FESEM images of SLA surfaces before and after PDA/ CPP modification(a) SLA; (b) SLA/PDA/CPP

图2 改性前后SLA表面XPS全谱图及C1s高分辨率图谱

Fig. 2 XPS wide scan spectra (A, B) and high resolution spectra of C1s (a, b) of SLA surfaces before (A, a) and after (B, b) PDA/CPP modification

图3 SLA(A)和SLA/PDA/CPP(B)在SBF溶液分别浸泡1、3、5、7 d后表面的FESEM照片

Fig. 3 FESEM images of SLA (A) and SLA/PDA/CPP (B) immersed in SBF solution for 1, 3, 5 and 7 d

图4 改性前后SLA表面细胞粘附率测试结果, **表示两组数据之间存在显著统计学差异(p<0.01)

Fig. 4 Cell Attachment rates on SLA surfaces before and after PDA/CPP modification, with ** indicating a highly statistically significant difference (p<0.01)

图5 改性前后SLA表面细胞增殖率测试结果, **表示两组数据之间存在显著统计学差异(p<0.01)

Fig. 5 Cell proliferation on SLA surfaces before and after PDA/CPP modification, with ** indicating a highly statistically significant difference (p<0.01)

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SLA表面PDA/CPP复合涂层的制备及其性能研究

Preparation and Property of PDA/CPP Bilayer on SLA Surfaces

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