新型神经调控用TiO2-Au光电转换纳米复合物性能研究

doi:10.15541/jim20160458

无机材料学报 ›› 2017, Vol. 32 ›› Issue (5): 550-554.DOI: 10.15541/jim20160458

新型神经调控用TiO₂-Au光电转换纳米复合物性能研究

蒋莉^1,2, 刘佳男¹, 范文培^1,2, 刘艳颜^1,2, 倪大龙^1,2, 步文博¹

(1. 中国科学院上海硅酸盐研究所, 高性能陶瓷和超微结构国家重点实验室, 上海200050; 2. 中国科学院大学, 北京100049)

收稿日期:2016-08-08 出版日期:2017-05-20 网络出版日期:2017-05-02
作者简介:蒋莉. E-mail: jennyforyoung@126.com

Performance Study of TiO₂-Au Photoelectric Nanocomposites for Novel Neuromodulation

JIANG Li^1,2, LIU Jia-Nan¹, FAN Wen-Pei^1,2, LIU Yan-Yan^1,2, NI Da-Long^1,2, BU Wen-Bo¹

(1. State Key Laboratory of High Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China; 2. University of the Chinese Academy of Sciences, Beijing100049, China)

Received:2016-08-08 Published:2017-05-20 Online:2017-05-02
About author:JIANG Li(1990–), female, candidate of Master degree. E-mail: jennyforyoung@126.com
Supported by:
Foundation item: National Natural Science Foundation of China (51372260)

摘要/Abstract

摘要：

本文提出了一种基于光电转换纳米复合物(NCs)的新型神经调控方式。NCs是TiO₂纳米晶表面连接金纳米粒子的复合物, 可产生光电流并有效引发神经细胞去极化。在405 nm光照射下, NCs产生的光电流比单一TiO₂纳米晶产生的光电流强度显著提高。PC12细胞上的膜电位荧光探针和钙离子荧光探针测试结果显示, 在405 nm光照下, 经NCs处理的细胞发生去极化。在活体抗癫痫实验中进一步证明, NCs产生的光电流可明显减轻斑马鱼的癫痫发作。本研究结果表明NCs可进行神经调控, 对神经疾病的治疗具有重要意义。

关键词: 纳米复合物, 光电转换, 神经调控, 癫痫

Abstract:

A novel noninvasive yet highly efficient neuromodulation method via photoelectric nanocomposites (NCs) was proposed. The NCs were constructed by Au nanoparticles-attached TiO₂ nanocrystals. Upon 405 nm light exposure, the photocurrent was enhanced when the pure TiO₂ nanocrystals were modified with Au nanoparticles. It is evidenced that effective depolarization of NCs-treated neurons takes place upon 405 nm light illumination, certified by fluorescent dye test on PC12 cells. Furthermore, the NCs could remarkably remit the epilepsy seizures of zebrafishes, indicating that the NCs are potential candidates for curing neurological disorders.

Key words: nanocomposites, photoelectric, neuromodulation, epilepsy

中图分类号:

TQ174

蒋莉, 刘佳男, 范文培, 刘艳颜, 倪大龙, 步文博. 新型神经调控用TiO₂-Au光电转换纳米复合物性能研究[J]. 无机材料学报, 2017, 32(5): 550-554.

JIANG Li, LIU Jia-Nan, FAN Wen-Pei, LIU Yan-Yan, NI Da-Long, BU Wen-Bo. Performance Study of TiO₂-Au Photoelectric Nanocomposites for Novel Neuromodulation[J]. Journal of Inorganic Materials, 2017, 32(5): 550-554.

图/表 4

Fig. 1 Schematic illustration of NCs depolarizing neurons upon 405 nm light exposure

Fig. 2 Representative TEM images of (a) TiO2 nanocrystals, (b) Au nanoparticles, and (c) NCs. Inset of (a) optical image of TiO2 nanocrystals dispersed in chloroform, (c) magnifying TEM image of NCs. (d) UV/Vis absorption spectrum of NCs; (e) photocurrent responses of the NCs and TiO2 electrode to a cyclic “on-off” illumination of 405 nm light. Measurements were performed on electrochemical workstation with Pt as counter electrode, Ag/AgCl as reference electrode, and 0.2 mol/L Na2SO4 as electrolyte 405 nm light from Xenon lamp (300 W) was used as the excitation source

Fig. 3 Real-time fluorescence intensity variation of Di-8-ANEPPS (a) and Cal-520 (c) in PC12 cells observed by CLSM. Max fluorescence intensity variation of Di-8-ANEPPS (b) and Cal-520 (d) in PC12 cells; data represents mean ± SD (n = 3)

Fig. 4 (a) Schematic illustration of NCs anti-epileptic performance investigation on zebrafishes. The motion trails in 1 h of (b1) normal zebrafishes, epileptic zebrafishes treated with PBS (b2), NCs (b3), illumination (b4), both NCs and illumination (b5). The red, green and black lines represent motion trails with speed of >20 mm/s, 4-20 mm/s, and <4 mm/s, respectively. (c) Distance of zebrafishes moving with speed of >20 mm/s; normal zebrafishes (I), epileptic zebrafishes treated with PBS (II), NCs (III), illumination (IV), both NCs and illumination (V). Data represents mean ± SD (n = 30)

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新型神经调控用TiO₂-Au光电转换纳米复合物性能研究

Performance Study of TiO₂-Au Photoelectric Nanocomposites for Novel Neuromodulation

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