B2O3-SiO2-Na2O缓释抑菌剂的合成及性能表征

doi:10.15541/jim20160395

摘要/Abstract

摘要：

硼酸盐具有抑菌和抑藻性能, 研究采用高温熔融法合成了以硼为抑菌因子的B₂O₃-SiO₂-Na₂O缓释抑菌剂。运用X射线衍射分析(XRD)、扫描电镜(SEM)及红外光谱(FTIR)表征抑菌剂的理化性能, 采用电感耦合等离子体发射光谱仪(ICP-AES)测定缓释液中硼元素的浓度并分析抑菌剂的缓释特性, 分别以大肠杆菌和金黄色葡萄球菌为受试细菌, 以抑菌圈和抑菌率为指标评价药剂的抑菌效果。结果表明, 抑菌剂均呈无定形非晶形态, 内部[BO₃]和[BO₄]基团的含量不只与B₂O₃有关, 也受SiO₂的影响; 随着B₂O₃含量的降低, 抑菌剂表面结构逐渐致密, 缓释速率降低。随着缓释液中硼浓度的升高, 抑菌剂的抑菌作用增强, 且对金黄色葡萄球菌的抑制效果优于大肠杆菌。其中B₂O₃含量为55wt%的B₂O₃-SiO₂-Na₂O缓释抑菌剂的抑菌效果最佳, 这为硼系缓释抑菌剂的继续研究及工业化应用提供了参考依据。

关键词: 硼, 缓释, 抑菌作用

Abstract:

High temperature melting method has been used to synthesize B₂O₃-SiO₂-Na₂O controlled-release antibacterial agent on account of the functions that borate can inhibit the growth of bacteria and algae. The new inhibitor, a solid agent with controlled-release capability in water, is different from those liquid and gaseous antibacterial agents. In this study, the physicochemical properties of the inhibitor were characterized by XRD, SEM and FTIR. The concentrations of boron in solution were determined by using ICP-AES to determine the release capability of the inhibitor. Moreover the experiments of inhibitory circle and inhibitory rate were carried out to evaluate the antibacterial effects of the inhibitor on Escherichia Coli and Staphylococcus Aureus. The results indicate that the inhibitors present amorphous structure, with the content of [BO₃] and [BO₄] groups in the internal structure being affected by the amounts and the ratio of B₂O₃ and SiO₂. The agent surface is denser while the release rate decreases with the decrease of B₂O₃ amount. Furthermore, the antibacterial effect enhances with the increase of boron concentration. S. Aureus is more sensitive to the inhibitor than E. Coli. The inhibitor containing 55wt% of B₂O₃gets the best antibacterial effect , which exhibits better controlled-release than the others. The present results provide a reference for further research and industrial application of the antibacterial agent.

Key words: boron, controlled-release, antibacterial effect

中图分类号:

TQ174

史蕊蕊, 李萌, 裴元生. B₂O₃-SiO₂-Na₂O缓释抑菌剂的合成及性能表征[J]. 无机材料学报, 2017, 32(5): 529-534.

SHI Rui-Rui, LI Meng, PEI Yuan-Sheng. Synthesis and Characterizations of B₂O₃-SiO₂-Na₂O Controlled-release Antibacterial Agent[J]. Journal of Inorganic Materials, 2017, 32(5): 529-534.

图/表 8

表1 B2O3-SiO2-Na2O抑菌剂各组分的含量

Table 1 Composition of the B2O3-SiO2-Na2O inhibitor

Inhibitor	B₂O₃/wt%	Na₂O/wt%	SiO₂/wt%
1	70	12.4	17.6
2	65	11.5	23.5
3	60	10.6	29.4
4	55	9.8	35.2

图1 不同抑菌剂的XRD图谱

Fig. 1 XRD patterns of different inhibitors (a) Inhibitor-1; (b) Inhibitor-2; (c) Inhibitor-3; (d) Inhibitor-4

图2 不同抑菌剂的SEM照片

Fig. 2 SEM images for different inhibitors (a) Inhibitor-1; (b) Inhibitor-2; (c) Inhibitor-3; (d) Inhibitor-4

图3 不同抑菌剂的红外光谱图

Fig. 3 FTIR spectra of different inhibitors (a) Inhibitor-1; (b) Inhibitor-2; (c) Inhibitor-3; (d) Inhibitor-4

表2 硼硅酸盐材料中各基团的红外吸收光谱特征振动

Table 2 Characteristic vibration reflected by infrared absorption spectrum of each group in borosilicate materials

Wavenumber/cm^-1	Corresponding characteristic vibration ascription
3423-3426	H-O stretching vibration peak
1413-1424	[BO₃] anti symmetrical stretching vibration peak
1071-1076	[BO₄] anti symmetrical stretching vibration peak
923-930	[BO₄] anti symmetrical stretching vibration peak
686-696	[BO₃] flexural vibration peak
451-466	Si-O-Si flexural vibration peak

图4 不同抑菌剂在常温下缓释出硼的量(mg/mL)

Fig. 4 Concentration of controlled-releasing boron of different inhibitors at room temperature (mg/mL)

表3 抑菌剂对大肠杆菌和金黄色葡萄球菌的抑菌圈/mm

Table 3 Inhibitory circle of E. Coli and S. Aureus against inhibitor/mm

Inhibitor	Boron /(mg·mL^-1)		E. coli			S. aureus
Inhibitor	Boron /(mg·mL^-1)	0.5	1	1.5	2	0.5	1	1.5	2
1	Diameter	<10	10±0.5	12±0.5	15±0.5	11±0.5	17±0.5	19±0.5	30±0.5
1	Sensitivity	–	+	+	+	+	++	++	+++
2	Diameter	<10	10±0.5	11±0.5	17±0.5	13±0.5	17±0.5	20±0.5	23±0.5
2	Sensitivity	–	+	+	++	+	++	++	+++
3	Diameter	<10	11±0.5	13±0.5	18±0.5	14±0.5	17±0.5	19±0.5	22±0.5
3	Sensitivity	–	+	+	++	+	++	++	+++
4	Diameter	<10	14±0.5	14±0.5	16±0.5	12±0.5	16±0.5	20±0.5	30±0.5
4	Sensitivity	–	+	+	++	+	++	+++	+++

图5 不同抑菌剂对大肠杆菌和金黄色葡萄球菌的抑菌率

Fig. 5 Inhibitory rate of E. Coli and S.Aureus against different inhibitors

参考文献 27

[1]	GUNES Y.Inhibition of boric acid and sodium borate on the biological activity of microorganisms in an aerobic biofilter.Environmental Technology, 2013, 34(9): 1117-1121.
[2]	LIU H, CHEN X Q, PEI Y S.Acute toxic effects of boron compounds on chlorella vulgaris.Research of Environmental Sciences, 2015, 28(12): 1887-1894.
[3]	GENTZ M C, GRACE J K.A review of boron toxicity in insects with an emphasis on termites.Journal of Agricultural and Urban Entomology, 2006, 23(4): 201-207.
[4]	THEVEN M F, TONDI G, PIZZI A.Environmentally friendly wood preservative system based on polymerized tannin resin-boric acid for outdoor applications.Maderas-Ciencia Y Tecnologia, 2010, 12(3): 253-257.
[5]	LUCACEL R C, RADU T, TATAR A S, et al.The influence of local structure and surface morphology on the antibacterial activity of silver-containing calcium borosilicate glasses.Journal of Non-Crystalline Solids, 2014, 404: 98-103.
[6]	LIU J C.Study on inorganic antibacterial borosilicate glass and its performance.Journal of Zhejiang Fashion Institute of Technology, 2009, 04: 63-66.
[7]	KRIZKOVA S, RYANT P, KRYSTOFOVA O, et al.Multi-instrumental analysis of tissues of sunflower plants treated with silver(I) ions-plants as bioindicators of environmental pollution.Sensors, 2008, 8(1): 445-463.
[8]	LESNIKOWSKI Z J.Recent developments with boron as a platform for novel drug design.Expert Opinion on Drug Discovery, 2016, 11(6): 569-578.
[9]	PEREVRA A M, GONZALEZ M R, ROSATO V G, et al.A-type zeolite containing Ag+/Zn2+ as inorganic antifungal for waterborne coating formulations.Progress in Organic Coatings, 2014, 77(1): 213-218.
[10]	HAN Y, TIAN Y M, PING W U, et al.Compound preparation of biocide in reclaimed water used as circulating cooling water.China Water and Wastewater, 2013, 29(21): 107-110.
[11]	QIU Z Z, YANG L, LI D L, et al.Application of ozone technological process for recirculated cooling water treatment in central air-conditioning system of subway stations.Water Purification Technology, 2011, 30(1): 53-57.
[12]	HOFFMANN M.Ozone-bromine treatment-water treatment in public pools without chlorine-a new standard?Ozone Science and Engineering, 2015, 37(5): 456-466.
[13]	MILLER H C, WYLIE J, DEJEAN G, et al.Reduced efficiency of chlorine disinfection of naegleria fowleri in a drinking water distribution biofilm.Environmental Science and Technology, 2015, 49(18): 11125-11231.
[14]	SEZONOV G, JOSELEAU-PETIT D, DARI R.Escherichia coli physiology in luria-bertani broth.Journal of Bacteriology, 2007, 189(23): 8746-8749.
[15]	ZHAO Y N, WU Y L, ZHANG W L.Study on phase separation and crystallization of Na2O-B2O3-SiO2-TiO2 glass.Bulletin of the Chinese Ceramic Society, 2005, 06: 70-72, 119.
[16]	KAUR K, SINGH K J, ANAND V.Structural properties of Bi2O3- B2O3-SiO2-Na2O glasses for gamma ray shielding applications.Radiation Physics and Chemistry, 2015, 120: 63-72.
[17]	WANG W H.The nature and properties of amorphous matter.Progress in Physics, 2013, 33(5): 177-351.
[18]	TANG Y, JIANG Z, SONG X.NMR, IR and Raman spectra study of the structure of borate and borosilicate glasses.Journal of Non-Crystalline Solids, 1989, 112(1-3): 131-135.
[19]	VERHOEF A H, HARTOG H W.Structure and dynamics of alkali borate glasses-a molecular dynamics study.Journal of Non-Crystalline Solid, 1995, 182: 235-247.
[20]	ZHANG X, LI J M, LI C Z.The research of IR and crystal structure in borax.Guangdong Chemical Industry, 2010, 37(08): 77-79.
[21]	DOWEIDAR H, EL-DAMRAWI G, AL-ZAIBANI M.Distribution of species in Na2O-CaO-B2O3 glasses as probed by FTIR.Vibrational Spectroscopy, 2013, 68(9): 91-95.
[22]	EL-EGILI K.Infrared studies of Na2O-B2O3-SiO2 and Al2O3- Na2O-B2O3-SiO2 glasses.Physica B-Condensed Matter, 2003, 325(02): 340-348.
[23]	万军鹏. 浮法硼硅酸盐平板玻璃组成、结构与性能的研究. 武汉: 武汉理工大学博士学位论文, 2008.
[24]	REGEV-SHOSHANI G, KO M, MILLER C, et al.Slow release of nitric oxide from charged catheters and its effect on biofilm formation by Escherichia coli.Antimicrobial Agents and Chemotherapy, 2010, 54(1): 273-279.
[25]	LIU J, WANG H Y, ZHAO S J.Determination of the antibacterial activity of galla chinensis on pathogen vibrio of pseudosciaena crocea by oxford plate assay system.Ocean Science, 2009, 33: 44-47.
[26]	SEVERIN A I, KOKEGUCHI S, KATO K.Chemical composition of Eubacterium nodatum cell wall peptidoglycan.Archives of Microbiology, 1989, 151(4): 353-358.
[27]	SAMMAN S, NAGHII M R, WALL P L, et al.The nutritional and metabolic effects of boron in humans and animals.Biological Trace Element Research, 1998, 66(1/2/3): 227-235.

B₂O₃-SiO₂-Na₂O缓释抑菌剂的合成及性能表征

Synthesis and Characterizations of B₂O₃-SiO₂-Na₂O Controlled-release Antibacterial Agent

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