多巴胺辅助牙本质沉积羟基磷灰石研究

doi:10.15541/jim20150262

摘要/Abstract

摘要：

本研究报道了一种新颖的、模拟口腔环境的仿生修复牙齿的方法。利用具有良好生物相容性的多巴胺在牛牙牙本质表面自聚合形成聚多巴胺层(PDA)使牙本质表面功能化, 进而诱导羟基磷灰石从模拟唾液中向牙本质表面沉积。XPS、XRD、SEM和EDS表征分析表明: 聚多巴胺层的涂覆有利于牙本质表面羟基磷灰石晶体的结晶生长, 可提高羟基磷灰石再矿化层与牙本质表面的结合力; 多巴胺溶液浓度为2 mg/mL时, 牙本质表面聚多巴胺层的沉积效果最佳。

关键词: 仿生材料, 多巴胺自聚合, 牙齿再矿化, 结晶生长

Abstract:

A novel and biomimetic restoration method for teeth by soaking in a simulated oral environment was reported. A biocompatible polydopamine (PDA) layer was deposited on the surface of dentin via dopamine polymerization to functionalize the surface to induce deposition of hydroxyapatite crystals. The samples were characterized by means of XPS, XRD, SEM and EDS. Results show that the coating of polydopamine layer induces the growth of hydroxyl apatite crystal on dentin surface, and improve the binding force between the hydroxyl apatite layer and the dentin surface. The optimized concentration of dopamine solution is 2 mg/mL for the uniform deposition of PDA layer on the dentin surface.

Key words: biomimetic materials, polymerization of dopamine, mineralization of teeth, crystal growth

中图分类号:

R314

许金妹, 刘新玲, 高彦峰. 多巴胺辅助牙本质沉积羟基磷灰石研究[J]. 无机材料学报, 2016, 31(1): 95-99.

XU Jin-Mei, LIU Xin-Ling, GAO Yan-Feng. Effect of Dopamine on Hydroxyapatite Deposition for Dental Restoration[J]. Journal of Inorganic Materials, 2016, 31(1): 95-99.

图/表 8

图1 在不同浓度多巴胺溶液中处理后的牙本质表面SEM照片

Fig. 1 SEM images of the dentin surfaces after being soaked in dopamine solution at different concentrations,(a, b) 0 mg/mL; (c, d) 1 mg/mL; (e, f) 2 mg/mL; (g, h) 3 mg/mL

图2 空白牙本质(a)和沉积有聚多巴胺层的牙本质(b)表面的XPS全谱图谱

Fig. 2 XPS wide scan spectra of 37% H3PO4-etched dental slices (a) and the polydopamine coated dentin surface (b)

表1 空白牙本质、多巴胺和表面沉积有多巴胺牙本质的表面元素含量

Table 1 Element content of the dentin, dopamine and enamel-PDA surfaces

	Dentin	Dopamine (theoretical value)	Dentin -PDA
C1s/ at%	7.2	8	11.75
N1s/ at%	2.3	1	2.1
N/C	0.319	0.125	0.179

图3 不同方式处理后的牙本质的XRD谱图: (a)空白牙本质; (b)无聚多巴胺涂层的牙本质再矿化; (c)含聚多巴胺涂层的牙本质再矿化

Fig. 3 XRD patterns of the sample surfaces of 37% H3PO4- etched dental slices (a) and precipitates on the dental surface without (b) or with (c) polydopamine coating after remineralization for 7 d

图4 不同方式处理后的牙本质表面再矿化的SEM照片

Fig. 4 SEM images of HAP remineralization of the dentin surface coated with (a, b) or without (c, d) polydopamine after mineralization for 7 d

表2 羟基磷灰石、含和不含PDA涂层的牙本质表面再矿化物的元素原子百分比

Table 2 Elements atomic percentage of the surface of hydroxyapatite and precipitates on the dentin surface with or without polydopamine coating after remineralization for 7 days

Element	Surface with PDA	Surface without PDA	HAP
O/at%	69.10	52.88	62.50
P/at%	13.00	17.40	12.50
Ca/at%	17.90	23.81	20.83

图5 不同方式处理后的牙片断面SEM照片

Fig. 5 SEM images of the transverse sections of 37% H3PO4-etched dental slices (a) and the preciptates on the enamel surface without (b) or with (c) polydopamine coating after remineralization for 7 d

图6 不同方式处理后的牙本质表面再矿化并超声后的SEM照片

Fig. 6 SEM images of dentin surface treated by ultrasonic wave which were remineralized with (a, b) or without (c, d) polydopamine coating after mineralization for 7 d

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