磷酸镁/PBS/小麦蛋白复合骨修复材料

doi:10.15541/jim20150076

摘要/Abstract

摘要：

采用共沉淀法合成磷酸镁, 将磷酸镁(MP)、聚丁二酸丁二醇酯(PBS)和小麦蛋白(WP)进行复合, 制备出MP/PBS/WP复合骨修复材料。通过体外降解、生物活性以及细胞培养等实验对复合材料的理化性能及细胞相容性进行了研究。结果显示: MP/PBS/WP复合材料在Tris-HCl缓冲液中浸泡10 d后, 溶液pH从7.4上升至7.51, 浸泡12 w后, 其降解率达到58.43wt%; 复合材料在模拟体液中浸泡10 d后, 其表面形成磷灰石层; 复合材料能促进MC3T3-E1细胞的增殖与分化。结果表明: MP/PBS/WP复合材料具有优良的降解性、生物活性和细胞相容性, 有望成为一种新型骨修复材料。

关键词: 磷酸镁, PBS, 小麦蛋白, 生物复合材料, 骨修复

Abstract:

Magnesium phosphate was prepared through coprecipitation method, and biocomposite containing magnesium phosphate(MP), polybutylene succinate (PBS) and wheat protein (WP) was fabricated. In vitro degradability, bioactivity and cell responses to MP/PBS/WP composite were investigated. The results showed that the pH value changed from 7.4 to 7.51 after the MP/PBS/WP composite being soaked in Tris-HCl buffer solution for 10 d. The weight loss of the composite reached 58.43wt% after soaking for 12 w. Apatite layer could form on the composite surfaces after soaking in SBF solution for 10 d, indicating good bioactivity. In addition, the MP/PBS/WP composite could promote proliferation and differentiation of MC3T3-E1 cells. All data from this study show that the MP/PBS/WP composite has good degradability, bioactivity and cytocompatibility, showing a potential to be used as a new biomaterial for bone repair.

Key words: magnesium phosphate, PBS, wheat protein, biocomposite, bone repair

中图分类号:

TB383

王泉翔, 邬迎阳, 董谢平, 马旭辉, 魏杰. 磷酸镁/PBS/小麦蛋白复合骨修复材料[J]. 无机材料学报, 2015, 30(9): 957-962.

WANG Quan-Xiang, WU Ying-Yang, DONG Xie-Ping, MA Xu-Hui, WEI Jie. Magnesium Phosphate/PBS/Wheat Protein Biocomposite for Bone Repair[J]. Journal of Inorganic Materials, 2015, 30(9): 957-962.

图/表 8

图1 磷酸镁的透射电镜照片(a)和PBS、MP/PBS复合材料及MP/PBS/WP复合材料的宏观照片(b)

Fig. 1 TEM image of magnesium phosphate (a) and photos of PBS, MP/PBS composite and MP/PBS/WP composite (b)

图2 PBS(a)、MP/PBS复合材料(b)、MP/PBS/WP复合材料(c)、MP(d)和WP(e)的XRD图谱

Fig. 2 XRD patterns of PBS (a), MP/PBS composite (b), MP/PBS/WP composite (c), MP (d), and WP (e)

图3 PBS(a)、MP/PBS复合材料(b)和MP/PBS/WP复合材料(c) 在Tris-HCl缓冲液浸泡不同时间后的失重率(A)和pH变化(B)

Fig. 3 Weight loss (A) and pH change (B) of PBS (a), MP/PBS composite (b) and MP/PBS/WP composite (c) soaked in Tris-HCl solution for different time

图4 PBS(a)、MP/PBS复合材料(b)和MP/PBS/WP复合材料(c)在SBF溶液中浸泡10 d后的扫描电镜照片

Fig. 4 SEM images of surface morphology of PBS (a), MP/PBS composite (b), and MP/PBS/WP composite (c) after soaking in SBF for 10 d

图5 MP/PBS复合材料和MP/PBS/WP复合材料在SBF溶液中浸泡10 d后的EDS图谱

Fig. 5 EDS spectra of MP/PBS composite and MP/PBS/WP composite after soaking in SBF for 10 d

图6 MP/PBS复合材料(a)和MP/PBS/WP复合材料(b)在SBF中浸泡不同时间后, 溶液中的Ca、Mg、P离子浓度的变化

Fig. 6 Ion concentrations changes in SBF after MP/PBS composite (a) and MP/PBS/WP composite (b) immersion for different time

图7 MC3T3-E1细胞在PBS(a)、MP/PBS复合材料(b) 和MP/PBS/WP复合材料(c)上培养7 d后的激光共聚焦显微照片

Fig. 7 CLSM of cytoskeleton stained by FITC and DAPI after MC3T3-E1 cells cultured on PBS (a), MP/PBS composite (b) and MP/PBS/WP composite (c) for 7 d

图8 MC3T3-E1细胞在PBS(a)、MP/PBS复合材料(b)、MP/PBS/WP 复合材料(c)上培养不同时间后的细胞增殖率(A)和ALP活性(B)

Fig. 8 Cell proliferation (A) and ALP activity (B) of MC3T3-E1 cells cultured on PBS (a), MP/PBS composite (b) and MP/PBS/WP composite (c) for different time

参考文献 17

[1]	YE X, CAI S, DOU Y, et al.Bioactive glass-ceramic coating for enhancing the in vitro corrosion resistance of biodegradable Mg alloy.Applied Surface Science, 2012, 259: 799-805.
[2]	DU R L, CHANG J.Preparation and characterization of Zn and Mg doped bioactive glasses. Journal of Inorganic Material, 2004, 19(6): 1353-1358.
[3]	WU F, WEI J, GUO H, et al.Self-setting bioactive calciummagnesium phosphate cement with high strength and de gradability for bone regeneration.Acta Biomaterialia, 2008, 4(6): 1873-1884.
[4]	LIU Z S, LIU C S.Progress in research on magnesium phosphate cement as inorganic binder for bone repair.Materials Review, 2000, 14(5): 29-32.
[5]	JBILOU F, JOLY C, GALLAND S, et al.Biodegradation study of plasticised corn flour/poly(butylene succinate-co-butylene adipate) blends.Polymer Testing, 2013, 32(8): 1565-1575.
[6]	DOREZ G, TAGUET A, FERRY L, et al.Phosphorous compounds as flame retardants for polybutylene succinate/flax biocomposite: additive versus reactive route.Polymer Degradation and Stability, 2014, 201: 152-159.
[7]	XU H L, CAI S B, SELLERS A, et al.Electrospun ultrafine fibrous wheat glutenin scaffolds with three-dimensionally random organization and water stability for soft tissue engineering. Journal of Biotechnology, 2014, 184: 179-186.
[8]	GÓMEZ M D, PARTAL P, MARTÍNEZ I, et al. Rheological behaviour and physical properties of controlled-release gluten-based bioplastics.Bioresource Technology, 2009, 100(5): 1828-1832.
[9]	REZWAN K, CHEN Q Z, BLAKER J J, et al.Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering.Biomaterials, 2006, 27: 3413-3431.
[10]	LIU X, RAHAMAN M N, HILMAS G E, et al.Mechanical properties of bioactive glass(13-93) scaffolds fabricated by robotic deposition for structural bone repair.Acta Biomaterialia, 2013, 9(6): 7025-7034.
[11]	CHEN S H, LEI M, XIE X H, et al.PLGA/TCP composite scaffold incorporating bioactive phytomolecule icartin for enhancement of bone defect repair in rabbits.Acta Biomaterialia, 2013, 9(5): 6711-6722.
[12]	VELARD F, BRAUX J, AMEDEE J, et al.Inflammatory cell response to calcium phosphate biomaterial particles: an overview.Acta Biomaterialia, 2013, 9(2): 4956-4963.
[13]	ZHONG K, VASUDEVAN T V, Somasundaran P.Floatability of apatites of different type and origin: role of surface area and porosity.International Journal of Minerral Processing, 1993, 38(3/4): 177-188.
[14]	HUTMACHER D W.Scaffolds in tissue engineering bone and carilage.Biomaterials, 2000, 21(24): 2529-2543.
[15]	ELLI L, DOLFINI E, BARDELLA M T.Gliadin cytotoxicity and in vitro cell cultures.Toxicology Letters, 2003, 146(1): 1-8.
[16]	QIAO Y Q, ZHANG W J, TIAN P, et al.Stimulation of bone growth following zinc incorporation into biomaterials.Biomaterials, 2014, 35(25): 6882-6897.
[17]	ZHANG T, WU X N, HUANG H Y, et al.The beneficial influence of microarc oxidation-coated magnesium alloy on the adhesion, proliferation and osteogenic differentiation of bone marrow stromal cells.Materials Letters, 2014, 137: 362-365.