聚多巴胺修饰钛表面纳米载银及其抗菌和细胞相容性

doi:10.15541/jim20140162

摘要/Abstract

摘要：

通过多巴胺自聚合在钛表面构建了仿生聚多巴胺(PDA)膜层, 有利于类骨羟基磷灰石在钛表面的沉积, 体现了良好的生物活性。利用聚多巴胺的螯合效应及还原性, 将纳米银颗粒载入聚多巴胺修饰钛表面; 利用场发射扫描电镜(FE-SEM)、X射线光电子能谱(XPS)、显微激光拉曼光谱(Raman)和石墨炉原子吸收光谱(GF-AA5)对聚多巴胺/纳米银修饰钛表面的银粒径、含量及离子释放进行表征。采用杀菌率和表面细菌粘附对聚多巴胺/纳米银修饰钛表面的体外抗菌性能进行检测, 研究结果表明: 纳米银对金黄色葡萄球菌具有较强的杀菌能力, 且MC3T3-E1细胞早期粘附和增殖结果证实本研究得到的聚多巴胺/纳米银修饰钛表面具有良好的体外细胞相容性。

关键词: 钛, 聚多巴胺, 纳米银颗粒, 抗菌性能, 细胞相容性

Abstract:

A bioinspired polydopamine (PDA) layer was deposited on titanium surface by simply dipping the substrate into an alkaline dopamine solution. The in vitro bioactivity of the polydopamine coated titanium was assessed by incubation in simulated body fluids (SBF). The results showed that surface-anchored catecholamine moieties in polydopamine enriched the interface with calcium ions, facilitating the deposition of hydroxyapatite. Silver nanoparticles (AgNPs) were metallised on the PDA-grafted titanium surface in mild aqueous environments. About 14% silver release as Ag⁺ ions were found after 5 d when the surfaces were exposed to deionized water. The AgNPs showed an efficient microbicidal activity against Staphylococcus Aureus bacterial strains, while not significantly affecting osteoblast cells (MC3T3-E1) viability.

Key words: titanium, polydopamine, silver nanoparticles, antibacterial property, cytocompatibility

中图分类号:

TB331

谭英, 谭帼馨, 宁成云, 容锡沧, 张余, 周蕾. 聚多巴胺修饰钛表面纳米载银及其抗菌和细胞相容性[J]. 无机材料学报, 2014, 29(12): 1320-1326.

TAN Ying, Tan Guo-Xin, NING Cheng-Yun, RONG Xi-Cang, ZHANG Yu, ZHOU Lei. Bioinspired Polydopamine Functionalization of Titanium Surface for Silver Nanoparticles Immobilization with Antibacterial Property[J]. Journal of Inorganic Materials, 2014, 29(12): 1320-1326.

图/表 6

图1 酸处理钛(a)、纳米结构化钛(b)和聚多巴胺修饰钛(c)表面的扫描电镜照片及水的接触角(d)

Fig. 1 SEM images (a-c) and water contact angle (d) of Ti (a), pTi (b) and Ti-PDA(c)

图2 钛样品在模拟体液中矿化5 d后的电镜照片、能谱和红外图谱

Fig. 2 SEM images, EDS spectra (a-c) and FTIR spectra (d) of Ti (a), pTi (b) and Ti-PDA (c) after 5 d in simulated body fluid

图3 聚多巴胺修饰钛表面纳米载银SEM(a)、XPS(b)、Raman(c)及银离子溶出(d)

Fig. 3 SEM (a), XPS (b), Raman spectrum (c) and release of Ag+ (d) of Ti-PDA-Ag

图4 Ti(a)、pTi(b)、Ti-PDA(c)和Ti-PDA-Ag(d)样品对金黄色葡萄球菌的抑菌能力照片

Fig. 4 Typical photographs of re-cultivated Staphylococcus Aureus colonies on agar Ti (a), pTi (b), Ti-PDA (c) and Ti-PDA-Ag (d)

图5 样品表面金黄色葡萄球菌在Ti(a)、pTi(b)、Ti-PDA(c)和Ti-PDA-Ag(d)上粘附的SEM照片

Fig. 5 SEM images of the Ti (a), pTi (b), Ti-PDA (c) and Ti-PDA-Ag (d) surfaces after incubation with Staphylococcus Aureus for 6 h

图6 样品表面MC3T3-E1细胞在Ti(a)、pTi(b)、Ti-PDA(c)、Ti-PDA-Ag(d)上粘附的SEM照片及MTT检测结果(e)

Fig. 6 SEM images showing the morphology of MC3T3-E1 cells after 12 h of culture on Ti (a), pTi(b), Ti-PDA (c), Ti-PDA -Ag (d) and MC3T3-E1 proliferation measured using MTT after incubation for 1, 3, 5 and 7 d (n=5, p< 0.05 ) (e) *represents significant difference

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