溶胶-凝胶生物活性玻璃的结构特征及其对糖尿病创面修复作用的研究

doi:10.3724/SP.J.1077.2013.12232

无机材料学报 ›› 2013, Vol. 28 ›› Issue (1): 103-108.DOI: 10.3724/SP.J.1077.2013.12232

溶胶-凝胶生物活性玻璃的结构特征及其对糖尿病创面修复作用的研究

林才^1,2,3, 毛葱^1,2, 李玉莉^1,2, 张娟娟^1,2, 苗国厚^1,2, 陈晓峰^1,2

(1．国家人体组织功能重建工程技术研究中心, 广州 510006; 2. 华南理工大学材料科学与工程学院, 广州 510640; 3. 温州医学院附属第一医院烧伤科, 温州 325000)

收稿日期:2012-04-13 修回日期:2012-06-09 出版日期:2013-01-10 网络出版日期:2012-12-20
作者简介:林才(1969–), 男, 副主任医师. E-mail: lincai0577@hotmail.com
基金资助:
国家重点基础研究发展计划(973计划)(2011CB606204, 2012CB619100);国家自然科学基金(50830101, 51172073);华南理工大学中央高校基本科研业务费(2012ZP0001);浙江省自然科学基金重点项目(Z2080985)

Structural Properties and Wound Healing Effect of the Sol-Gel Bioactive Glass on Diabetic Skin Wounds

LIN Cai^1,2,3, MAO Cong^1,2, LI Yu-Li^1,2, ZHANG Juan-Juan^1,2, MIAO Guo-Hou^1,2, CHEN Xiao-Feng^1,2

(1. The National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China; 2. The School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China; 3. Department of Burns, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China)

Received:2012-04-13 Revised:2012-06-09 Published:2013-01-10 Online:2012-12-20
About author:LIN Cai. E-mail: lincai0577@hotmail.com
Supported by:
973 Program (2011CB606204, 2012CB619100);National Natural Science Foundation of China (50830101, 51172073);Fundamental Research Funds for the Central Universities (2012ZP0001);Zhejiang Provincial Natural Science Foundation of China (Z2080985)

摘要/Abstract

摘要：

采用熔融法和溶胶-凝胶法分别制备了生物活性玻璃45S5和SGBG, 通过SEM、BET及XRD等方法对它们的微观结构进行了表征, 并建立了SD大鼠糖尿病皮肤创面模型, 通过对创面愈合时间、创面愈合率以及HE染色分析, 探讨了生物活性玻璃对促进糖尿病难愈创面愈合的效果。结果表明, 与凡士林组相比, 生物活性玻璃组能加速创面愈合, 且SGBG具有纳米结构, 其比表面积更大, 与45S5相比能缩短糖尿病创面的愈合时间, 提高愈合速度。组织学分析表明生物活性玻璃能促进肉芽组织的生长。由此表明生物活性玻璃能促进大鼠糖尿病创面的修复, 且具有纳米结构的SGBG效果更好。

关键词: 生物活性玻璃, 溶胶-凝胶, 创面修复, 糖尿病

Abstract:

The 45S5 bioactive glass (45S5) was prepared by a melting process, while sol-gel bioactive glass (SGBG) was obtained by Sol-Gel method. The bioactive glasses were characterized by SEM、BET and XRD. Then the wound healing effect was investigated through the wound healing time, wound healing rate and histology examination. The results indicate that the bioactive glass can lessen the wound healing time and increase the healing rates of diabetic rats. Compared with the 45S5, SGBG can promote wound healing of diabetic rats more quickly and efficiently due to the larger surface area and nanostructure. Histological examination shows that bioactive glasses promote the proliferation of fibroblasts and growth of granulation tissue. All results suggest that bioactive glass can accelerate the recovery of skin wounds and SGBG with nanostructure has a better healing effect in diabetes- impaired models.

Key words: bioactive glass, Sol-Gel method, wound healing, diabetes

中图分类号:

TQ174

林才, 毛葱, 李玉莉, 张娟娟, 苗国厚, 陈晓峰. 溶胶-凝胶生物活性玻璃的结构特征及其对糖尿病创面修复作用的研究[J]. 无机材料学报, 2013, 28(1): 103-108.

LIN Cai, MAO Cong, LI Yu-Li, ZHANG Juan-Juan, MIAO Guo-Hou, CHEN Xiao-Feng. Structural Properties and Wound Healing Effect of the Sol-Gel Bioactive Glass on Diabetic Skin Wounds[J]. Journal of Inorganic Materials, 2013, 28(1): 103-108.

图/表 8

图1 两种生物活性玻璃45S5 (a)和SGBG (b)的SEM照片

Fig. 1 SEM images of 45S5 (a), SGBG (b)

图2 两种生物活性玻璃的XRD图谱

Fig. 2 XRD patterns of 45S5 and SGBG

图3 45S5和SGBG生物活性玻璃的吸附-脱附等温曲线(a, c)和孔径分布图(b, d)

Fig. 3 Adsorption-desorption isotherms (a, c) and pore size distributions patterns (b, d) of 45S5 and SGBG Bioglass

图4 两种生物活性玻璃浸泡液pH值随时间变化曲线

Fig. 4 pH values of SBF in different immersion times for two types of the bioglass

图5 糖尿病大鼠术后各时相点创面愈合情况

Fig.5 Wound healing image following treatments with SGBG, 45S5 and vaseline

图6 各组的创面愈合时间(a)和各组各时相点的创面愈合率(b)

Fig. 6 (a) Wound healing times of three groups and (b) Wound healing rates of the diabetic rats treated by different methods (n=8)

图7 术后7和14 d各组的HE染色图

Fig. 7 Representative images of H&E staining of wound sections at 7 d and 14 d after surgery. All pictures were taken under 10 times magnification using a Zeiss microscope (bar represents 400 µm). Arrows indicate blood vessels

Table 1 The stages time of granulation tissue formation in different groups (d)

Granulation tissue	Bioactive glass/d	Vaseline
Prophase	0-2	0-4
Early stage	2-4	6-10
Peaking stage	4-6	10-16
Reducing stage	8-12	16-18
Vanishing stage	After 12	After 18

参考文献 20

[1]	Brem Harold, Tomic-canic Marjana. Cellular and molecular basis of wound healing in diabetes. Journal of Clinical Investigation, 2007, 117(5): 1219-1222.
[2]	Hench L L. Bioactive materials: the potential for tissue regeneration. Journal of Biomedical Materials Research, 1998, 41(4): 511-518.
[3]	Hencl L L, Splinter R J, Allen W C, et al. Bonding mechanisms at the interface of ceramic prosthetic materials. Journal of Biomedical Materials Research,1971, 5(6):117-141.
[4]	Gatti A, Valdr G, Andersson H. Analysis of the in vivo reactions of a bioactive glass in soft and hard tissue. Biomaterials,1994, 15(3): 208-212.
[5]	Verrier S, Blaker J J, Hench L L, et al. PDLLA/Bioglass® composites for soft-tissue and hard-tissue engineering: an in vitro cell biology assessment. Biomaterials, 2004, 25(15): 3013-3021.
[6]	Day R M. Bioactive glass stimulates the secretion of angiogenic growth factors and angiogenesis in vitro. Tissue Engeering, 2005, 11(5): 768-777.
[7]	Day R M, Boccaccini A R, Hench L L, et al. Assessment of polyglycolic acid mesh and bioactive glass for soft-tissue engineering scaffolds. Biomaterials, 2004, 25(27): 5857-5966.
[8]	Keshaw H, Forbes A, Day R M. Release of angiogenic growth factors from cells encapsulated in alginate beads with bioactive glass. Biomaterials, 2005, 26(19): 4171-4179.
[9]	Xynos I D, Edgar A J, Buttery L D K, et al. Gene-expression profiling of human osteoblasts following treatment with the ionic products of Bioglass® 45S5 dissolution. Journal of Biomedical Materials Research, 2001, 55(2): 151-157.
[10]	Mortazavi V, Naahrkhalaji M M, Fathi M H, et al. Antibacterial effects of Sol-Gel-derived bioactive glass nanoparticle on aerobic bacteria. Journal of Biomedical Materials Research Part A, 2010, 94A(1):160-168.
[11]	Hench L L. Genetic design of bioactive glass. Journal of the European Ceramic Society, 2009, 29(7): 1257-1265.
[12]	Chen Xiao-feng, Meng Yong-chun, Li Yu-li, et al. Investigation on bio-mineralization of melt and Sol-Gel derived bioactive glasses. Applied Surface Science, 2008, 255(2): 562-564.
[13]	Hench L L, West J K. The Sol-Gel process. Chemical Reviews, 1990, 90(1): 33-72.
[14]	Hench L L. Bioceramics: from concept to clinic. Journal of the American Ceramic Society, 1991, 74(7): 1487-1510.
[15]	Ye Yue-Li, Wang Shi-Hui, Sun Ju-Mei, et al. Healing effect of inorganic elements on second degree burn wounds. Clinical Medicine, 2007, 27(7): 48-49.
[16]	Zhou Lai-sheng, Liao Zhen-jiang, Zhang Qin, et al. Bio-inductive effects of inorganic elements on skin wound healing. Chinese Journal of Burns, 2005, 21(5): 363-366.
[17]	Epstein F H, Singer A J, Clark R A F. Cutaneous wound healing. New England Journal of Medicine, 1999, 341(10): 738-746.
[18]	Blakytny R, Jude E. The molecular biology of chronic wounds and delayed healing in diabetes. Diabetic Medicine, 2006, 23(6): 594-608.
[19]	Maruyama K, Asai J, Li M, et al. Decreased macrophage number and activation lead to reduced lymphatic vessel formation and contribute to impaired diabetic wound healing. The American Journal of Pathology, 2007, 170(4):1178-1191.
[20]	Gurtner G C, Werner S, Barrandon Y, et al. Wound repair and regeneration. Nature, 2008, 453(7193): 314-321.

溶胶-凝胶生物活性玻璃的结构特征及其对糖尿病创面修复作用的研究

Structural Properties and Wound Healing Effect of the Sol-Gel Bioactive Glass on Diabetic Skin Wounds

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 20

相关文章 15

编辑推荐

Metrics

本文评价

[1]	吴锐, 张敏慧, 金成韵, 林健, 王德平. 光热核壳TiN@硼硅酸盐生物玻璃纳米颗粒的降解和矿化性能[J]. 无机材料学报, 2023, 38(6): 708-716.
[2]	唐洁吟, 王刚, 刘聪, 邹学农, 陈晓峰. 微纳米生物活性玻璃诱导牙本质再矿化研究[J]. 无机材料学报, 2022, 37(4): 436-444.
[3]	施吉翔, 翟东, 朱敏, 朱钰方. 生物活性玻璃-二氧化锰复合支架的制备与表征[J]. 无机材料学报, 2022, 37(4): 427-435.
[4]	陈铖, 丁晶鑫, 王会, 王德平. 掺钕介孔硼硅酸盐生物活性玻璃陶瓷骨水泥的制备与性能表征[J]. 无机材料学报, 2022, 37(11): 1245-1258.
[5]	朱子旻, 张敏慧, 张轩宇, 姚爱华, 林健, 王德平. 硼硅酸盐生物活性玻璃在直流电场下的体外矿化性能[J]. 无机材料学报, 2021, 36(9): 1006-1012.
[6]	林子扬, 常宇辰, 吴章凡, 包荣, 林文庆, 王德平. 不同模拟体液对硼硅酸盐生物活性玻璃基骨水泥矿化性能的影响[J]. 无机材料学报, 2021, 36(7): 745-752.
[7]	满鑫, 吴南, 张牧, 贺红亮, 孙旭东, 李晓东. Lu₂O₃-MgO纳米粉体合成及其复相红外透明陶瓷制备[J]. 无机材料学报, 2021, 36(12): 1263-1269.
[8]	杨丛纲, 米乐, 冯爱虎, 于洋, 孙大志, 于云. KH-560改性SiO₂绝缘薄膜的制备及性能研究[J]. 无机材料学报, 2021, 36(12): 1343-1348.
[9]	常宇辰, 林子扬, 谢昕, 吴章凡, 姚爱华, 叶松, 林健, 王德平, 崔旭. 基于介孔硼硅酸盐生物活性玻璃微球的可注射复合骨水泥[J]. 无机材料学报, 2020, 35(12): 1398-1406.
[10]	朱本必,张旺,张志坚,章建忠,IMRAN Zada,张荻. 光热增强光催化性能二氧化钛(B)/玻纤布复合研究[J]. 无机材料学报, 2019, 34(9): 961-966.
[11]	张少丹, 包维维, 马海萍. Cu ²⁺、Tb ³⁺共掺杂BaZrO₃高近红外反射颜料的制备及其性能研究[J]. 无机材料学报, 2019, 34(6): 599-604.
[12]	刘茜, 周真真. 低温活化合成含硅氮氧化物材料研究进展[J]. 无机材料学报, 2018, 33(2): 129-137.
[13]	赵慧月, 王晓栋, 冯建斌, 刘源, 黄吉辰, 沈军. NH₃/HTMS气相法改性SiO₂增透膜的耐环境稳定性[J]. 无机材料学报, 2018, 33(11): 1219-1224.
[14]	何飞, 李亚, 骆金, 方旻翰, 赫晓东. 具有气凝胶结构特征的C/SiO₂和C/SiC复合材料研究进展[J]. 无机材料学报, 2017, 32(5): 449-458.
[15]	路淑娟, 王唱, 赵博文, 汪浩, 刘晶冰, 严辉. PEG改性氧化钨薄膜的电致变色特性[J]. 无机材料学报, 2017, 32(2): 185-190.