包埋载药微球的羟基磷灰石/聚氨酯复合组织工程支架的研究

doi:10.3724/SP.J.1077.2011.01073

无机材料学报 ›› 2011, Vol. 26 ›› Issue (10): 1073-1077.DOI: 10.3724/SP.J.1077.2011.01073

包埋载药微球的羟基磷灰石/聚氨酯复合组织工程支架的研究

刘浩怀¹, 张建华¹, 许庆陵¹, 张利², 李玉宝²

(1. 广州大学化学化工学院, 分析测试中心, 广州510006; 2. 四川大学纳米生物材料研究中心, 分析测试中心, 成都610064)

收稿日期:2010-11-25 修回日期:2011-01-12 出版日期:2011-10-20 网络出版日期:2011-09-20
基金资助:
国家863计划项目(2007AA03Z328766); 广州大学新苗计划

Studies on Hydroxyapatite/Polyurethane Scaffold Containing Drug-loaded Microspheres for Bone Tissue Engineering

LIU Hao-Huai¹, ZHANG Jian-Hua¹, XU Qing-Ling¹, ZHANG Li², LI Yu-Bao²

(1. School of Chemistry and Chemical Engineering, Analytical and Testing Center, Guangzhou University, Guangzhou 510006, China; 2. The Research Center for Nano-biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, China)

Received:2010-11-25 Revised:2011-01-12 Published:2011-10-20 Online:2011-09-20
Supported by:
863 Program (2007AA03Z328766); New Telents Project of Guangzhou University

摘要/Abstract

摘要： 研制具有药物缓释功能的骨组织工程支架, 对载药微球包埋于羟基磷灰石/聚氨酯(HA/PU)支架中的药物缓释体系进行了可行性研究. 首先将盐酸环丙沙星作为模型药物, 包裹于乙基纤维素(EC)微球中, 然后将EC微球与HA/PU材料进行复合, 制备了抗生素药物缓释支架. 结果显示EC微球均匀地分布在HA/PU支架基质中, 未对支架的开孔结构和孔隙形貌构成影响. 与单纯将药物载入HA/PU支架中相比, 复合载药EC微球的HA/PU支架的初期药物暴释明显降低, 药物缓释时间延长. 体外药物释放实验和抑菌实验结果表明, 该载药微球支架具有良好的药物缓释功能和抑菌性能, 是一种集骨修复和治疗于一体的新型组织工程支架材料.

关键词: 聚氨酯, 支架, 微球, 药物缓释, 乙基纤维素

Abstract: To explore and develop scaffold for bone regeneration or tissue engineering with the capacity of controlled drug delivery, the feasibility of hydroxyapatite/polyurethane (HA/PU) scaffold containing drug-loaded microspheres for controlled drug delivery system was demonstrated. Ciprofloxacin hydrochlorid as a model drug was encapsulated in ethyl cellulose (EC) microspheres, which were subsequently incorporated into HA/PU composite scaffold to generate an antibiotic drug delivery system. The results show that EC microspheres are uniformly distributed in the HA/PU scaffold matrix and display no significant effect on the pore structure of the scaffold. Compared with incorporating ciprofloxacin hydrochlorid into scaffolds directly, embedding microspheres into scaffolds significantly reduces the initial burst drug release and extends the release time of drug delivery. In vitro drug delivery tests and antibacterial activity tests prove that drug-loaded microsphere/scaffold system has good drug delivery properties and effective antibacterial properties. These results suggest that the novel drug-loaded microsphere/ scaffold composites developed in this study is a good candidate scaffold with the function of bone repair and infection treatment for bone tissue engineering.

Key words: polyurethane, scaffold, microspheres, drug delivery, ethyl cellulose

中图分类号:

R318

刘浩怀, 张建华, 许庆陵, 张利, 李玉宝. 包埋载药微球的羟基磷灰石/聚氨酯复合组织工程支架的研究[J]. 无机材料学报, 2011, 26(10): 1073-1077.

LIU Hao-Huai, ZHANG Jian-Hua, XU Qing-Ling, ZHANG Li, LI Yu-Bao. Studies on Hydroxyapatite/Polyurethane Scaffold Containing Drug-loaded Microspheres for Bone Tissue Engineering[J]. Journal of Inorganic Materials, 2011, 26(10): 1073-1077.

参考文献

[1] Tabata Y. Significance of release technology in tissue engineering. Drug Discovery Today, 2005, 10(23/24): 1639-1646.

[2] 楼伟建, 章辉, 韩钢. 缓慢释放bFGF壳聚糖多孔支架的构建及对细胞生长的影响. 中国生物医学工程学报, 2007, 26(3): 441-444.

[3] Habraken W, Wolke J, Mikos A, et al. PLGA microsphere/calcium phosphate cement composites for tissue engineering: in vitro release and degradation characteristics. Journal of Biomaterials Science, Polymer Edition, 2008, 19(9): 1171-1188.

[4] Liu W, Griffith M, Li F. Alginate microsphere-collagen composite hydrogel for ocular drug delivery and implantation. Journal of Materials Science: Materials in Medicine, 2008, 19(11): 3365-3371.

[5] Dass C, Walker T, Kalle W, et al. A microsphere-liposome (microplex) vector for targeted gene therapy of cancer. II. In vivo biodistribution study in a solid tumor model. Drug Delivery, 2000, 7(1): 15-19.

[6] Lee J, Kim S, Kwon I, et al. Effects of a chitosan scaffold containing TGF-β1 encapsulated chitosan microspheres on in vitro chondrocyte culture. Artificial Organs, 2004, 28(9): 829-839.

[7] Rowe R C, Kotaras A D, White E F T. An evaluation of the plasticizing efficiency of the dialkyl phthalates in ethyl cellulose films using the torsional braid pendulum. International Journal of Parmaceutics, 1984, 22(1): 57-62.

[8] Porter S C. The effect of additives on the properties of an aqueous film coating. Pharm.Tech., 1980(4): 67-75.

[9] Hypp-l- R, Husson I, Sundholm F. Evaluation of physical properties of plasticized ethyl cellulose films cast from ethanol solution Part I. International Journal of Pharmaceutics, 1996, 133(1/2): 161-170.

[10] 刘浩怀, 张利, 左奕, 等. 脂肪族聚氨酯/磷灰石复合材料的原位制备及性能. 功能材料, 2010, 41(2): 241-244.

[11] Li Yubao, De Wijn J, Klein C P A T, et al. Preparation and characterization of nanograde osteoapatite-like rod crystals. Journal of Materials Science: Materials in Medicine, 1994, 5(5): 252-255.

[12] Guan J J, Sacks M S, Beckman E J, et al. Synthesis, characterization, and cytocompatibility of efastomeric, biodegradable poly(ester-urethane) ureas based on poly(caprolactone) and putrescine. Journal of Biomedical Materials Research, 2002, 61(3): 493-503.

[13] Hou Q, Grijpma D W, Feijen J. Porous polymeric structures for tissue engineering prepared by a coagulation, compression moulding and salt leaching technique. Biomaterials, 2003, 24(11): 1937-1947.

[14] 董志红, 李玉宝, 张利, 等(DONG Zhi-Hong, et al). 软骨修复用 HA/PU 多孔支架材料的制备与表征. 无机材料学报(Journal of Inorganic Materials), 2007, 22(6): 1255-1258.

[15] Julien M, Khairoun I, Legeros R Z, et al. Physico- chemical–mechanical and in vitro biological properties of calcium phosphate cements with doped amorphous calcium phosphates. Biomaterials, 2007, 28(6): 956-965.

[16] Ilyina T, Romanova Y, Gintsburg A. Biofilms as a mode of existence of bacteria in external environment and host body: the phenomenon, genetic control, and regulation systems of development. Russian Journal of Genetics, 2004, 40(11): 1189-1198.

[17] Richards R, Harris L, Schneider E, et al. Antiseptics and antibiotics on implants. Injury, 2006, 37(2): S113-S116.

包埋载药微球的羟基磷灰石/聚氨酯复合组织工程支架的研究

Studies on Hydroxyapatite/Polyurethane Scaffold Containing Drug-loaded Microspheres for Bone Tissue Engineering

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	施吉翔, 翟东, 朱敏, 朱钰方. 生物活性玻璃-二氧化锰复合支架的制备与表征[J]. 无机材料学报, 2022, 37(4): 427-435.
[2]	张航, 韩坤原, 董兰兰, 李祥. DLP打印β-磷酸三钙/纳米黏土复合支架的制备与表征[J]. 无机材料学报, 2022, 37(10): 1116-1122.
[3]	吴重草, 郇志广, 朱钰方, 吴成铁. 3D打印HA微球支架的制备与表征[J]. 无机材料学报, 2021, 36(6): 601-607.
[4]	郭小炜, 李玉妍, 陈南春, 王秀丽, 解庆林. 负载二甲酸钾缓释抗菌微球的构建[J]. 无机材料学报, 2021, 36(2): 181-187.
[5]	潘碧宸,任鹏禾,周特军,蔡圳阳,赵小军,周宏明,肖来荣. 树脂基复合材料表面隔热涂层的组织与性能研究[J]. 无机材料学报, 2020, 35(8): 947-952.
[6]	董少杰,王旭东,沈国芳,王晓虹,林开利. 生物陶瓷支架的功能改性及应用研究进展[J]. 无机材料学报, 2020, 35(8): 867-881.
[7]	胡亚萍, 田正芳, 朱敏, 朱钰方. 喷雾干燥可控制备生物玻璃微球及其体外生物活性研究[J]. 无机材料学报, 2020, 35(11): 1268-1276.
[8]	高龙, 张赵文斌, 常江. 生物玻璃/聚乳酸多孔微球的制备及其作为细胞载体的研究[J]. 无机材料学报, 2020, 35(10): 1163-1168.
[9]	肖文谦,张静,李克江,邹新宇,蔡昱东,李波,刘雪,廖晓玲. 荔枝状CaCO₃@HA/Fe₃O₄磁性介孔多级微球的制备[J]. 无机材料学报, 2019, 34(9): 925-932.
[10]	李波, 郝文, 文晓刚. 半空心/实心ZnMn₂O₄微球: 合成及锂离子电池性能[J]. 无机材料学报, 2018, 33(3): 307-312.
[11]	陈希亮, 陈庆华, 庄颖, 颜廷亭. KGM/明胶/nano HAP椎间盘纤维环组织工程支架的制备与研究[J]. 无机材料学报, 2018, 33(1): 60-66.
[12]	辛晨, 齐鑫, 朱敏, 赵世昌, 朱钰方. 三维打印羟基磷灰石晶须增强复合骨修复支架[J]. 无机材料学报, 2017, 32(8): 837-844.
[13]	鲍艳, 康巧玲. 中空TiO₂微球的制备及其对聚丙烯酸酯薄膜保温性能的影响[J]. 无机材料学报, 2017, 32(6): 581-586.
[14]	余素春, 喻莹, 戴红莲. 明胶微球/磷酸镁基骨水泥复合药物缓释体系的构建[J]. 无机材料学报, 2017, 32(6): 655-660.
[15]	秦冬昱, 何晓龙, 聂秋林, 殷好勇, 袁求理. 氧化镍/镍/碳微球原位制备及葡萄糖传感性能[J]. 无机材料学报, 2017, 32(3): 313-318.