生物活性聚乳酸/SiO2-CaO复合薄膜的制备和表征

doi:10.3724/SP.J.1077.2011.11125

无机材料学报 ›› 2011, Vol. 26 ›› Issue (9): 998-1002.DOI: 10.3724/SP.J.1077.2011.11125

生物活性聚乳酸/SiO₂-CaO复合薄膜的制备和表征

李晋波^1,2, 刘宣勇², 李伟锋³, 朱剑豪⁴

(1. 湖南大学材料科学与工程学院, 长沙410082; 2. 中国科学院上海硅酸盐研究所, 高性能陶瓷和超微结构国家重点实验室, 上海 200050; 3. 苏州大学材料与化学化工学院, 苏州215123; 4. 香港城市大学物理与材料科学系, 香港)

收稿日期:2011-03-02 修回日期:2011-04-26 出版日期:2011-09-20 网络出版日期:2011-08-19
作者简介:LI Jin-Bo (1976-), male, candidate of PhD. E-mail: lijinbo_yin@163.com
基金资助:
Shanghai Science and Technology R&D Fund (1052nm06600); National Natural Science Foundation of China (51071168); Shanghai-Unilever Research and Development Fund (09520715200)

Preparation and Characterization of Bioactive Poly (Lactic Acid)/SiO₂-CaO Composite Membranes

LI Jin-Bo^1,2, LIU Xuan-Yong², LI Wei-Feng³, ZHU Jian-Hao⁴

(1. College of Materials Science and Engineering, Hunan University, Changsha 410082, China; 2. State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China; 3. College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China; 4. Department of Physics & Materials Science, City University of Hong Kong, Hong Kong, China)

Received:2011-03-02 Revised:2011-04-26 Published:2011-09-20 Online:2011-08-19
About author:LI Jin-Bo (1976-), male, candidate of PhD. E-mail: lijinbo_yin@163.com
Supported by:
Shanghai Science and Technology R&D Fund (1052nm06600); National Natural Science Foundation of China (51071168); Shanghai-Unilever Research and Development Fund (09520715200)

摘要/Abstract

摘要： 以聚乳酸、正硅酸乙酯和硝酸钙为原料, 采用溶胶-凝胶方法制备了不同比例的聚乳酸/SiO₂-CaO多孔复合薄膜. 采用扫描电子显微镜和红外光谱仪对薄膜的微观结构和组成进行分析, 并利用表面Zeta电位测定仪对其表面电位进行表征, 通过模拟体液浸泡实验和MTT方法对薄膜的生物活性和细胞毒性进行评价. 结果表明: 复合薄膜在模拟体液中经过7d的浸泡, 薄膜表面形成了环状结构的类骨磷灰石层; 随着SiO₂-CaO含量增加, 复合薄膜表面微孔的孔径变小, 薄膜表面Zeta电位变负, 诱导类骨磷灰石沉积的能力增强; MTT实验证实复合薄膜对MG-63细胞没有毒性且有利于细胞的增殖.

关键词: 聚乳酸, SiO₂-CaO, Zeta表面电位, 生物活性, MTT

Abstract: A novel porous poly (lactic acid) (PLA)/SiO₂-CaO(n(SiO₂):n(CaO)=1:1) composite membrane is synthesized by Sol-Gel method. The microstructure and composition of the composite membranes are characterized by Fourier transform infrared (FTIR) spectroscope and scanning electron microscope (SEM). The surface zeta potential is monitored by the SurPASS electrokinetic analyzer. The bioactivity is evaluated by investigating the formation of apatite on the surface of the composite membranes in simulated body fluids and the cytotoxicity is evaluated by MTT experiments. After soaked in simulated body fluids for 7d, the surface of the PLA/SiO₂-CaO composite membranes is covered by an apatite layer with an orderly ring structure. The surface zeta potential of the composite membranes decreases with increasing SiO₂-CaO contents suggesting better bioactivity. The PLA/SiO₂-CaO composite membranes do not induce cytotoxcity to MG-63 cells and support cell proliferation.

Key words: PLA, SiO₂-CaO, Zeta potential, bioactivity, MTT

中图分类号:

TB332

李晋波, 刘宣勇, 李伟锋, 朱剑豪. 生物活性聚乳酸/SiO₂-CaO复合薄膜的制备和表征[J]. 无机材料学报, 2011, 26(9): 998-1002.

LI Jin-Bo, LIU Xuan-Yong, LI Wei-Feng, ZHU Jian-Hao. Preparation and Characterization of Bioactive Poly (Lactic Acid)/SiO₂-CaO Composite Membranes[J]. Journal of Inorganic Materials, 2011, 26(9): 998-1002.

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生物活性聚乳酸/SiO₂-CaO复合薄膜的制备和表征

Preparation and Characterization of Bioactive Poly (Lactic Acid)/SiO₂-CaO Composite Membranes

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