溶胶-凝胶生物活性玻璃与胶原复合多孔支架的生物相容性研究

doi:10.3724/SP.J.1077.2011.00869

无机材料学报 ›› 2011, Vol. 26 ›› Issue (8): 869-873.DOI: 10.3724/SP.J.1077.2011.00869

溶胶-凝胶生物活性玻璃与胶原复合多孔支架的生物相容性研究

韩雪^{1, 2}, 陈晓峰^{1, 2}, 孟永春^{1, 2, 3}, 周嘉安^{1, 2}, 林才^1,2,3, 姜晓锐⁴, 张鑫鑫⁴

(1．国家人体组织功能重建工程技术研究中心, 广州 510640; 2. 华南理工大学材料科学与工程学院, 广州 510640; 3. 温州医学院附属第一医院烧伤科, 温州 325000; 4. 南方医科大学南方医院创伤骨科, 广州 510515)

收稿日期:2010-10-12 修回日期:2010-11-26 出版日期:2011-08-20 网络出版日期:2011-07-14
作者简介:韩雪(1984-), 女, 硕士研究生. E-mail: hanxue1113@163.com
基金资助:
国家自然科学基金(50830101, 50732003, 51072055); 浙江省自然科学基金(Y407241)

Biocompatibility of the Composite Scaffold of Sol-Gel Bioactive Glass/Collagen

HAN Xue^{1, 2}, CHEN Xiao-Feng^{1, 2}, MENG Yong-Chun^{1, 2, 3}, ZHOU Jia-An^{1, 2}, LIN Cai^{1, 2, 3}, JIANG Xiao-Rui⁴, ZHANG Xin-Xin⁴

(1. National Engineering Research Center for Tissues Restoration and Reconstruction, Guangzhou 510640, China; 2. School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China; 3. Department of Burn, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China; 4. Department of Orthopaedics Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China)

Received:2010-10-12 Revised:2010-11-26 Published:2011-08-20 Online:2011-07-14
Supported by:
National Natural Science Foundation of China(50830101, 50732003, 51072055); Natural Science Foundation of Zhejiang Province (Y407241)

摘要/Abstract

摘要： 实验前期用冷冻干燥法合成一种溶胶?凝胶生物活性玻璃(BG/COL)与粗胶原纤维复合的组织工程支架. 本实验将支架与鼠骨髓间充质干细胞(rMSCs)共同培养, 评价支架材料的细胞相容性. 并将复合了成骨细胞的支架材料植入裸鼠皮下, 探讨其异位成骨的性能. 研究结果显示rMSCs可以在BG/COL多孔支架材料表面成功粘附、铺展、并向多孔支架内部迁移, 随着培养时间的延长, 双链DNA(ds DNA)数量增多, 细胞增殖情况与对照组差异明显. 将种在复合材料上的骨髓间充质干细胞诱导培养14d后切片染色, 其碱性磷酸酶(ALP)和钙素表达均呈强阳性. 体内植入实验的裸鼠健康状况良好, 伤口完全愈合, 6w后BG/COL周边及内部有骨组织和血管生成. 由此证明, 这种新型的复合多孔支架材料具有良好的生物相容性, 其复合了成骨细胞的组织工程骨具有良好的诱导成骨的性能, 因此这种材料是理想的应用于骨组织修复和再生的组织工程支架材料.

关键词: 生物活性玻璃, 骨髓间充质干细胞, 胶原, 生物相容性

Abstract: Biomimetic scaffold for bone tissue engineering was prepared by mixing Sol-Gel bioactive glass with type I collagen through freeze-drying technique. In vitro, the biocompatibility of the scaffold was investigated by observing the adhesive, proliferative and differential behaviors of the rat mesenchymal stem cells (rMSCs). In vivo, the composite scaffold seeded with osteoblasts was implanted subcutaneously into the immunodeficient mice for 6w. It was proved that the composite scaffold was non-cytotoxic and suitable for cells’ proliferation, which was confirmed by the increase of double stranded DNA (ds DNA). The differentiation of rMSCs on the composite scaffold was also observed by positive expressions of alkaline phosphatase (ALP) and osteocalcin after osteoinduction for 14d. The results of general and histological observation showed that cells successfully spread on the surface and migrated into the interior of the scaffold. Moreover, bone formation analysis of cell-scaffold constructs in vivo showed that bone tissue and blood vessels were regenerated both inside and on the border of the scaffold-stack. All results demonstrate the Sol-Gel bioactive glass-type I collagen scaffold with good biocompatibility and osteogenesis is a new ideal scaffold for bone tissue repair and regeneration.

Key words: bioglass, mesenchymal stem cell, collagen, biocompatibility

中图分类号:

R318

韩雪, 陈晓峰, 孟永春, 周嘉安, 林才, 姜晓锐, 张鑫鑫. 溶胶-凝胶生物活性玻璃与胶原复合多孔支架的生物相容性研究[J]. 无机材料学报, 2011, 26(8): 869-873.

HAN Xue, CHEN Xiao-Feng, MENG Yong-Chun, ZHOU Jia-An, LIN Cai, JIANG Xiao-Rui, ZHANG Xin-Xin. Biocompatibility of the Composite Scaffold of Sol-Gel Bioactive Glass/Collagen[J]. Journal of Inorganic Materials, 2011, 26(8): 869-873.

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溶胶-凝胶生物活性玻璃与胶原复合多孔支架的生物相容性研究

Biocompatibility of the Composite Scaffold of Sol-Gel Bioactive Glass/Collagen

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