预处理对构建丝素蛋白纤维/磷灰石仿生骨修复材料的影响

doi:10.3724/SP.J.1077.2011.00043

无机材料学报 ›› 2011, Vol. 26 ›› Issue (1): 43-48.DOI: 10.3724/SP.J.1077.2011.00043

预处理对构建丝素蛋白纤维/磷灰石仿生骨修复材料的影响

程新, 李延报, 陆春华, 李东旭, 许仲梓

南京工业大学材料科学与工程学院, 材料化学工程国家重点实验室, 南京 210009

收稿日期:2010-03-19 修回日期:2010-05-14 出版日期:2011-01-20 网络出版日期:2010-12-23
作者简介:程新(1987-), 女, 硕士研究生. E-mail: chengxin1104@126.com
基金资助:
国家自然科学基金(50802042); 江苏省自然科学基金(BK2008379); 材料化学工程国家重点实验室开放基金(KL09-6)

Effect of Pretreatment on Fabrication of Natural FibroinFiber/Apatite Composites Using Alternate Soaking Method

CHENG Xin, LI Yan-Bao, LU Chun-Hua, LI Dong-Xu, XU Zhong-Zi

State Key Laboratory of Materials-Oriented ChemicalEngineering, College of Materials Science and Engineering, Nanjing Universityof Technology, Nanjing 210009, China

Received:2010-03-19 Revised:2010-05-14 Published:2011-01-20 Online:2010-12-23
Supported by:
National Nature Science Foundation of China(50802042);Nature Science Foundation of Jiangsu(BK2008379); The State Key Laboratory of Materials-Oriented Chemical Engineering(KL09-6)

摘要/Abstract

摘要： 利用氯化钙-乙醇-水(n(CaCl₂):n(EtOH):n(H₂O)=1:2:8)三元溶液对丝素蛋白纤维预处理, 再采用交替浸渍法将其分别浸在钙溶液和磷溶液中交替浸渍、仿生矿化制备丝素蛋白纤维/磷灰石复合材料, 采用SEM、FTIR、XRD和TGA等技术研究了不同预处理时间对丝素蛋白纤维微结构和构建丝素蛋白纤维/磷灰石的影响. 结果表明, 预处理增加了丝素蛋白纤维表面粗糙程度及内部孔隙率, 并使钙离子与丝素蛋白纤维中的羧基、羟基和酰胺基等基团先发生作用, 可以有效地提高磷灰石在丝素蛋白表面的沉积量, 使得预处理后的丝素蛋白纤维与磷灰石的结合更均匀、密实, 获得了磷灰石晶体沿c轴取向生长的仿骨结构的复合材料.

关键词: 丝素蛋白纤维, 磷灰石, 生物矿化

Abstract: To develop biomimetic fibroin/apatite composites with bone-like structure byalternate soaking method, the degummed natural fibroin fibers were pretreatedby soaking into CaCl₂:EtOH:H₂O (<>n(CaCl₂):<>n(EtOH):<>n(H₂O)=1:2:8) solution for given time and then soaked into calciumsolution and phosphate solution, respectively, to fabricate fibroin/apatite composites. The effects of pretreatment time andthe alternate soaking times on fibroin microstructure and fibroin/apatitecomposites were examined by SEM, FTIR, XRD and TGA techniques. The pretreatmentusing CaCl₂:EtOH:H₂O(<>n(CaCl₂):<>n(EtOH):<>n(H₂O)=1:2:8) solution makes fibroin fiber exhibit rougher surface and higher internalporosity, which is beneficial to interaction of calcium ion and residue groupssuch as -COOH, –OHand -CONH- from fibroin, and then improvethe deposition of apatite on fibroin fibers. The homogeneous apatites withoriented <>c-axis were formed on the pretreated fibroin fibers. Moreover,there are more than 30wt% apatites deposited on fibroin fibers after alternatesoaking for more than 3 times. The fibroin/apatite composite has potential tobe applied as bone repair materials.

Key words: natural fibroin fiber, apatite, biomineralization

中图分类号:

TP174
R318

程新, 李延报, 陆春华, 李东旭, 许仲梓. 预处理对构建丝素蛋白纤维/磷灰石仿生骨修复材料的影响[J]. 无机材料学报, 2011, 26(1): 43-48.

LI Zhi-Hong, WU Ji-Min, HUANG Shu-Jie, GUAN Jing, ZHANGXi-Zheng. Effect of Pretreatment on Fabrication of Natural FibroinFiber/Apatite Composites Using Alternate Soaking Method[J]. Journal of Inorganic Materials, 2011, 26(1): 43-48.

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预处理对构建丝素蛋白纤维/磷灰石仿生骨修复材料的影响

Effect of Pretreatment on Fabrication of Natural FibroinFiber/Apatite Composites Using Alternate Soaking Method

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