骨碎补载入对磷酸钙骨水泥性能的影响及体外生物学评价

doi:10.3724/SP.J.1077.2011.00029

无机材料学报 ›› 2011, Vol. 26 ›› Issue (1): 29-37.DOI: 10.3724/SP.J.1077.2011.00029

骨碎补载入对磷酸钙骨水泥性能的影响及体外生物学评价

姜晓鑫¹, 屈树新², 林孙忠², 段可², 翁杰²

西南交通大学1. 生命科学与工程学院, 2.材料科学与工程学院, 材料先进技术教育部重点实验室, 成都 610031

收稿日期:2010-07-26 修回日期:2010-09-27 出版日期:2011-01-20 网络出版日期:2010-12-23
作者简介:姜晓鑫(1984-), 女, 硕士研究生．E-mail: whjxiaoxin@163.com
基金资助:
国家自然科学基金(30470484, 50975239); 教育部新世纪人才基金(NCET-05-0797)

Influenceof Drynaria on Physicochemical and <>in vitro Biological Properties ofCalcium Phosphate Cement

JIANGXiao-Xin¹, QU Shu-Xin², LIN Sun-Zhong², DUANKe², WENG Jie²

1. School of Life Science and Engineering, Southwest JiaotongUniversity, Chengdu 610031, China; 2. Key Lab of Advanced Technologies ofMaterials, Ministry of Education, School of Material Science and Engineering,Southwest Jiaotong University, Chengdu 610031, China

Received:2010-07-26 Revised:2010-09-27 Published:2011-01-20 Online:2010-12-23
Supported by:
National Nature Science Foundation ofChina(30470484, 50975239); New Century Excellent Talents in University(NCET-05-0797)

摘要/Abstract

摘要： 通过在湿法合成的二水磷酸氢钙膏体中加入中药骨碎补的提取物, 作为磷酸钙骨水泥(Calcium Phosphate Cement,CPC)原料之一, 分别制备0、5wt%、10wt%和15wt%的载骨碎补磷酸钙骨水泥. 采用Gilmore针、X射线衍射仪、红外光谱仪、万能材料试验机、扫描电子显微镜和紫外分光光度计研究载骨碎补CPC的理化性能和药物释放; 体外培养MC-3T3成骨细胞, 进行Alamar Blue和碱性磷酸酶检测, 研究载骨碎补CPC对成骨细胞增殖和分化的影响, 扫描电子显微镜观察细胞形貌. 结果表明: 随骨碎补浓度的增加, CPC凝结时间明显延长, 其抗压强度显著提高; 骨碎补促进初期CPC的水化, 却阻碍了α-磷酸三钙的转化, 且随骨碎补浓度增大作用愈明显, 骨碎补不影响CPC水化后的相成分; 含骨碎补CPC的微观形貌中出现片状和针状晶体, 结构较空白CPC更加致密; 药物释放分为突释和缓释两个阶段, 符合Higuchi基质扩散释放模型; 载骨碎补CPC对成骨细胞的作用呈剂量和时间依赖关系, 培养5d时浓度为5wt%和10wt%的CPC较明显地促进细胞增殖, 7d时载骨碎补CPC的细胞增殖较稳定, 细胞分化能力无显著性差异; 成骨细胞在载骨碎补CPC表面生长形态良好, 表明该材料具有较好的生物相容性.

关键词: 磷酸钙骨水泥, 骨碎补, 理化性能, 生物相容性

Abstract: The extract of drynaria,a traditional Chinese medical herb, was added into dicalcium phosphate dihydrate(DCPD) with concentration of 5wt%, 10wt% and 15wt% to form drug-loaded calciumphosphate cement (CPC). The effects of drynaria on the physichemical propertiesand drug release behavior of CPC were characterized by Gilmore needle tests,X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), compressivetests, scanning electron microscope (SEM) and UV-Vis spectrophotometry (UV-Vis).The effects of drynaria-loaded CPC on the morphology, proliferation, and differentiation of MC-3T3osteoblast were evaluated by SEM, Alamar blue assay, and alkaline phosphataseassay, respectively. It was found thatthe addition of drynaria increased the setting time and compressive strength ofCPC in dose-dependent manners. The addition of drynaria also accelerated theinitial hydration of CPC, but inhibited the subsequent conversion ofα-tricalcium phosphate, which were closely related to the concentration of drynaria.In comparison, the phases of CPC hydration products were not affected by drynaria.Drynaria-loaded CPC consisting of platyand acicular crystals were more compact than the control CPC. The drug releaseprofile exhibited an initial fast release stage followed by a slow releasestage, which was fit to the diffusion-controlledHiguchi model. The effect of drynaria-loaded CPC on osteoblastswas time- and dose-dependent. CPC loaded with 5wt% and 10wt% drynaria significantlypromoted osteoblast proliferation after culture for 5d (P<0.01). After cultured withdrynaria-loaded CPC for 7d, osteoblasts proliferated steadily. Osteoblasts cultured on drynaria-loaded CPCs and controlCPC showed similar differentiation. Osteoblasts with a normal morphology wereobserved on the surface of drynaria-loaded CPC, indicating an excellentbiocompatibility.

Key words: calcium phosphate cement, drynaria, physichemicalproperties, biocompatibility

中图分类号:

TB321

姜晓鑫, 屈树新, 林孙忠, 段可, 翁杰. 骨碎补载入对磷酸钙骨水泥性能的影响及体外生物学评价[J]. 无机材料学报, 2011, 26(1): 29-37.

JIANGXiao-Xin, QU Shu-Xin, LIN Sun-Zhong, DUANKe, WENG Jie. Influenceof Drynaria on Physicochemical and <>in vitro Biological Properties ofCalcium Phosphate Cement[J]. Journal of Inorganic Materials, 2011, 26(1): 29-37.

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骨碎补载入对磷酸钙骨水泥性能的影响及体外生物学评价

Influenceof Drynaria on Physicochemical and <>in vitro Biological Properties ofCalcium Phosphate Cement

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