载黄芪多糖羟基磷灰石的制备及生物学性能评价

doi:10.3724/SP.J.1077.2011.00022

无机材料学报 ›› 2011, Vol. 26 ›› Issue (1): 22-28.DOI: 10.3724/SP.J.1077.2011.00022

载黄芪多糖羟基磷灰石的制备及生物学性能评价

常立娜¹ ², 屈树新², 林孙忠², 段可², 翁杰²

西南交通大学1. 生命科学与工程学院; 2. 材料科学与工程学院, 材料先进技术教育部重点实验室, 成都 610031

收稿日期:2010-03-12 修回日期:2010-05-12 出版日期:2011-01-20 网络出版日期:2010-12-23
作者简介:常立娜(1985-), 女, 硕士研究生. E-mail: clndyx@163.com
基金资助:
国家自然科学基金(50975239, 30470484);教育部科学技术研究重点项目(109137)

Preparation and Biological Properties of HA Containing Astragalus Polysaccharides

CHANG Li-Na¹ ²,QU Shu-Xin², LIN Sun-Zhong², DUAN Ke², WENGJie²

1. School of Life Science and Engineering, Southwest Jiaotong University,Chengdu 610031, China; 2. School of Materials Science and Engineering, Key Lab of Advanced Technologiesof Materials, Ministry of Education, Southwest Jiaotong University, Chengdu610031, China

Received:2010-03-12 Revised:2010-05-12 Published:2011-01-20 Online:2010-12-23
Supported by:
National Nature Science Foundation of China (50975239,30470484); Important Project of Ministry of Education (109137)

摘要/Abstract

摘要： 室温湿法合成载不同浓度黄芪多糖(APS)的羟基磷灰石(HA/APS), 通过X射线衍射(XRD)表征APS对HA晶体结构、结晶度及晶粒尺寸的影响; 用透射电子显微镜(TEM)表征HA和HA/APS的晶体形貌, 用激光粒度仪和比表面积仪分别检测HA的粒度及比表面积;体外培养MC3T3-E1成骨细胞, 进行Alamar Blue、噻唑蓝(MTT)及碱性磷酸酶(ALP)检测, 光镜观察细胞形貌, 研究APS对成骨细胞作用的有效浓度范围及HA/APS对成骨细胞活性及分化的影响. 结果表明: 所载APS对HA晶体结构、结晶度和晶粒尺寸没有明显影响, 晶体形貌没有变化, HA平均粒径为1.17 μm, 比表面积为132.194 m²/g;APS对成骨细胞的作用呈剂量和时间依赖关系, 80~200 μg/mL促进细胞活性及ALP表达, HA/APS增强细胞活性, 细胞形貌完整. 因此, 0.5 g HA载入100~250 μg APS时明显促进成骨细胞活性, HA/APS具有作为临床骨缺损填充材料的潜能.

关键词: 羟基磷灰石, 黄芪多糖, 生物学性能

Abstract: Hydroxyapatite (HA) powders containing differentconcentrations of astragalus polysaccharides (APS) were synthesized by a wetchemical method. The effect of APS on the crystal structure, crystallinity andcrystal size of HA was characterized by XRD, the crystal morphologies of HA and HA/APS were characterized by TEM, the particle sizeand specific surface area of HA were detected by laser partical size distributionanalyzer, automated surface area and pore size analyzer, respectively. Furthermore,the effective concentration of APS on MC3T3-E1 osteoblasts, HA/APS onosteoblastic activity and differentiation was evaluated by Alamar blue, MTT andalkaline phosphatase assay. Cell morphologe was observed by light microscope.The results showed that the crystal structure, crystallinity andcrystal size of HA were not obviously effected by the presence of APS. Crystal morphologies of HA/APS was almost the samecompared with HA, the average particle size of HA was 1.17 μm and specificsurface area was 132.194 m²/g. The effect of APS on osteoblasts wastime and dose-dependent, APS concentrations within 80-200 μg/mL enhanced osteoblastic activity and ALPexpression, HA/APS enhanced osteoblastic activity and the osteoblasts hadintegrated morphology. It can be concluded that 0.5 g of HA containing 100-250 μm APS can promote osteoblastic activity. HA/APShas the promising potential as bone defect filling material for clinical applications.

Key words: hydroxyapatite, astragaluspolysaccharides, biological properties

中图分类号:

TB321

常立娜, 屈树新, 林孙忠, 段可, 翁杰. 载黄芪多糖羟基磷灰石的制备及生物学性能评价[J]. 无机材料学报, 2011, 26(1): 22-28.

CHANG Li-Na,QU Shu-Xin, LIN Sun-Zhong, DUAN Ke, WENGJie. Preparation and Biological Properties of HA Containing Astragalus Polysaccharides[J]. Journal of Inorganic Materials, 2011, 26(1): 22-28.

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载黄芪多糖羟基磷灰石的制备及生物学性能评价

Preparation and Biological Properties of HA Containing Astragalus Polysaccharides

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