Preparation and Characterization of Bioactive Glass-Manganese Dioxide Composite Scaffolds

doi:10.15541/jim20210264

Abstract

Abstract:

Inflammation in bone defect after being implanted scaffold is related to oxidative stress, which is caused mainly by higher concentration of hydrogen peroxide (H₂O₂). Manganese dioxide (MnO₂) can catalyze H₂O₂ decomposition to decrease excessive H₂O₂ in the surrounding environment of scaffolds. Furthermore, the oxygen (O₂) generated by the decomposition of H₂O₂ can alleviate the hypoxia caused by insufficient blood supply in bone defects, which is conducive to bone tissue regeneration. Here, a simple redox method was proposed to deposit MnO₂ particles on the surface of 3D printed bioactive glass (BG) scaffolds for the preparation of BG-MnO₂ composite scaffolds (BGM), which endows BG-MnO₂ scaffolds with the ability of H₂O₂ scavenging and O₂ supplying simultaneously. The results showed that the MnO₂ content deposited on the surface of BGM scaffolds was increased with the increase of potassium permanganate concentration in the reaction solution, and the compressive strength of BGM scaffolds was increased with the increase of MnO₂ content. However, porosity and degradation rate of these scaffolds with or without MnO₂remained the same. More importantly, BGM scaffolds can continuously catalyze the decomposition of H₂O₂ to produce O₂ in H₂O₂ environment. When BGM with different Mn content scaffolds (BMG5 and BGM9) catalyzed the decomposition of H₂O₂ to produce O₂ in 2 mmol/L H₂O₂ solution, the saturated oxygen concentration in the solution could reach 8.4 and 11 mg/L, respectively. In vitro cell experiments showed that BGM scaffolds could promote the proliferation and alkaline phosphatase activity of rabbit bone marrow mesenchymal stem cells. Hence, BGM scaffolds show great potential in bone regeneration.

Key words: bioactive glass scaffold, manganese dioxide, oxidative stress, 3D printing, bone tissue engineering

CLC Number:

TQ174

SHI Jixiang, ZHAI Dong, ZHU Min, ZHU Yufang. Preparation and Characterization of Bioactive Glass-Manganese Dioxide Composite Scaffolds[J]. Journal of Inorganic Materials, 2022, 37(4): 427-435.

Figures/Tables 7

Fig. 1 (A) Optical picture and (B) XRD patterns of BG and BGM scaffolds

Fig. 2 (A1, A2, B1, B2, C1, C2, D1, D2) SEM images and corresponding (A3, B3, C3, D3) EDS spectra of BG and BGM scaffolds (A1, A2, A3) BG scaffold; (B1, B2, B3) BGM1 scaffold; (C1, C2, C3) BGM5 scaffold; (D1, D2, D3) BGM9 scaffold

Fig. 3 (A) Porosities and (B) compressive strengths of BG and BGM scaffolds (* p< 0.05, ** p< 0.01)

Fig. 4 FT-IR spectra of BG and BGM scaffolds (A) before and (B) after soaking in SBF for 5 d

Fig. 5 (A) Degradation curves of BG and BGM scaffolds in Tris-HCl for 28 d, and (B) pH change curves of SBF after BG and BGM scaffolds soaking for 14 d

Fig. 6 (A) Dissolved oxygen levels in different concentrations of H2O2 solution after immersing BGM9 scaffolds, and (B) dissolved oxygen change curves in 2 mmol/L H2O2 solutions after immersing BGM scaffolds (cycle test for 3 times)

Fig. 7 (A) Proliferation and (B) ALP activity of rBMSCs on BG and BGM scaffolds

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