In situ Biomimetic Fabrication and Characterization of Nano-hydroxyapatite/ Chitosan Composite Microspheres

doi:10.15541/jim20140167

Abstract

Abstract:

To solve the problem that nano-hydroxyapatite (nHA) crystals aggregation and non-uniformly distribution in nano-hydroxyapatite/chitosan (CS) composite microspheres, nHA/CS microspheres were prepared through in situ biomimetic method in water-in-oil (W/O) emulsions. The physical and chemical properties of the composite microspheres were investigated by scanning electronic microscopy (SEM), X-ray energy-dispersive spectroscope (EDS), X-ray diffraction (XRD), fourier transform infrared spectroscope (FTIR) and laser particle size analyzer. The results indicate that the composite microspheres are spherical with average size of 8.62 μm and disperse uniformly. Moreover, the nHA crystals are dispersed uniformly within composite microspheres and bonded with CS matrix. The composite microspheres have great potential in bone tissue engineering and drug delivery system.

Key words: in situ biomimetic, nano-hydroxyapatite, chitosan, composite microspheres

CLC Number:

TB33

LI Jian, HAN Zhi-Jun, WEI Yan, NIU Lu-Lu, LIU Yu, LU Guo-Yun, HUANG Di. In situ Biomimetic Fabrication and Characterization of Nano-hydroxyapatite/ Chitosan Composite Microspheres[J]. Journal of Inorganic Materials, 2014, 29(12): 1327-1332.

Figures/Tables 7

Fig. 1 XRD patterns of pure CS microspheres (a), nHA/CS composite microspheres fabricated by in situ biomimetic method (b) and co-mixing method (c)

Fig. 2 FTIR spectra of pure CS microspheres (a), nHA/CS composite microspheres fabricated by in situ biomimetic method (b) and co-mixing method (c)

Fig. 3 SEM images of CS microspheres (a) and nHA/CS composite microspheres fabricated by co-mixing method: in the low mag-nification (b) and in the high magnification (c); EDS map-scanning analysis of Ca and P element (d, e) corresponding to (c)

Fig. 4 SEM images and EDS map-scanning analysis of nHA/CS composite microspheres fabricated by in situ biomimetic method: low magnification of the sample (a); higher magnification of the sample (b) and map-scanning analysis of Ca and P element (c, d) corresponding to the bottom rectangle region in (a)

Fig. 5 SEM images and EDS map-scanning analysis of the fracture surface of nHA/CS composite microspheres fabricated by in situ biomineralization method: low magnification of the sample (a); higher magnification of the sample (b) and map-scanning analysis of Ca and P element (c, d) corresponding to the pink rectangle region in (a)

Fig. 6 Size distribution of CS microspheres (a), nHA/CS composite microspheres fabricated by in situ biomimetic method (b) and co-mixing method (c)

Fig. 7 The growth process of nHA/CS composite microsphe-res fabricated in situ biomimetic method

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