中空羟基磷灰石微球作为rhBMP-2缓释载体的研究

doi:10.3724/SP.J.1077.2011.00974

无机材料学报 ›› 2011, Vol. 26 ›› Issue (9): 974-978.DOI: 10.3724/SP.J.1077.2011.00974

中空羟基磷灰石微球作为rhBMP-2缓释载体的研究

姚爱华¹, 徐为¹, 艾凡荣^1,3, 陈绮¹, 王德平^1,2, 黄文旵¹, 徐俊⁴

(1. 同济大学材料科学与工程学院, 上海 200092; 2. 先进土木工程材料教育部重点实验室, 上海 200092; 3. 南昌大学机电工程学院, 南昌330031; 4. 上海交通大学附属上海市第六人民医院, 上海 200233)

收稿日期:2010-10-30 修回日期:2010-12-15 出版日期:2011-09-20 网络出版日期:2011-08-19
基金资助:
上海市科委“创新行动计划”基础研究重点项目(08JC1419200); 同济大学青年优秀人才培养行动计划; 教育部留学回国人员科研启动基金; 国家自然科学基金(81000788)

Study on Hollow Hydroxyapatite Microspheres as Delivery System for Human Bone Morphogenetic Protein

YAO Ai-Hua¹, XU Wei¹, AI Fan-Rong^1,3, CHEN Qi¹, WANG De-Ping^1,2, HUANG Wen-Hai¹, Xu Jun⁴

(1. School of Materials Science and Engineering, Tongji University, Shanghai 200092, China; 2. Key Laboratory of Advanced Civil Engineering Materials, Ministry of Education, Tongji University, Shanghai 200092, China; 3. College of Mechanical and Electrical Engineering, Nanchang University, Nanchang 330031, China; 4. Shanghai Sixth People’s Hospital affiliated Shanghai JiaoTong University, Shanghai 200233, China)

Received:2010-10-30 Revised:2010-12-15 Published:2011-09-20 Online:2011-08-19
Supported by:
Key Project on Basic Research of Shanghai (08JC1419200); Program for Young Excellent Talent in Tongji University; Scientific Research Foundation of State Education Ministry for the Return Overseas Chinese Scholars; National Natural Science Foundation of China (81000788)

摘要/Abstract

摘要： 利用锂钙硼玻璃在磷酸盐溶液中的原位转化反应制备表面多孔且中空的羟基磷灰石(HA)微球, 将重组人骨形态发生蛋白2(rhBMP-2)装载到微球中, 研究了微球中rhBMP-2的体外缓释行为, 并采用体外细胞培养技术, 将载有rhBMP-2的微球和大鼠骨髓间充质干细胞(MSCs)一起培养, 测定细胞的碱性磷酸酶(ALP)活性, 并与单纯rhBMP-2的作用进行比较. 结果显示, 微球中所装载的rhBMP-2具有明显的缓释效应, 体外释放周期达到1000h以上, 该微球缓释系统具有一定的生物活性, 其作用效果优于单纯使用rhBMP-2.

关键词: 羟基磷灰石, 中空微球, 骨形态发生蛋白, 药物载体

Abstract: Hollow hydroxyapatite microspheres consisted of a hollow core and a porous shell were prepared by converting Li₂O-CaO-B₂O₃glass microspheres in dilute phosphate solution at 37℃. Human bone morphogenetic protein (rhBMP-2) was loaded in the resultant microspheres, and the in vitro release behavior of rhBMP-2 was studied. Furthermore, cell culture technique and ALP kit were used to investigate the effect of the released rhBMP-2 on cell activity. After incubation with rhBMP-2 loaded microspheres, the alkaline phosphatase (ALP) activity of the rat bone marrow mesenchymal stem cells (MSCs) was evaluated. It was found that rhBMP-2 could be released slowly from the hollow HA microspheres for an extended period time up to 1000h. The present delivery system established possessed higher biological activity as compared with pure rhBMP-2.

Key words: hydroxyapatite, hollow microsphere, bone morphogenetic protein, drug delivery

中图分类号:

TB321

姚爱华, 徐为, 艾凡荣, 陈绮, 王德平, 黄文旵, 徐俊. 中空羟基磷灰石微球作为rhBMP-2缓释载体的研究[J]. 无机材料学报, 2011, 26(9): 974-978.

YAO Ai-Hua, XU Wei, AI Fan-Rong, CHEN Qi, WANG De-Ping, HUANG Wen-Hai, Xu Jun. Study on Hollow Hydroxyapatite Microspheres as Delivery System for Human Bone Morphogenetic Protein[J]. Journal of Inorganic Materials, 2011, 26(9): 974-978.

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中空羟基磷灰石微球作为rhBMP-2缓释载体的研究

Study on Hollow Hydroxyapatite Microspheres as Delivery System for Human Bone Morphogenetic Protein

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