图1 FePS3块体的X射线衍射图谱(a)和扫描电镜照片(b)

Fig. 1 XRD pattern (a) and SEM image (b) of bulk FePS3

图2 FePS3 NSs的透射电镜照片(a), PEG修饰前(b)和修饰后(c)的平均水合粒径及其分散于PBS和DMEM中的照片(插图)

Fig. 2 TEM image (a) of FePS3 nanosheets (NSs) and hydrodynamic size of FePS3 nanosheets (NSs) before (b) and after (c) PEGylation with inset showing the picture of FePS3-PEG dispersed in PBS and in DMEM

图3 FePS3 NSs的 UV-Vis-NIR漫反射光谱和估算的带隙电势(插图)

Fig. 3 UV-Vis-NIR diffuse reflectance spectrum of FePS3 NSs with inset showing the estimated band gap potential

图4 660 nm激光照射下FePS3 NSs与DPBF的混合液(a)和纯DPBF溶液(b)的紫外-可见吸收光谱, 以及不同反应体系的ESR光谱图(c)

Fig. 4 UV-Vis absorption spectra of the mixture solution of FePS3 NSs mixed with DPBF (1,3-diphenylisobenzofuran) (a) and DPBF solution (b) under 660 nm laser irradiation, and ESR spectra of different reaction systems (c)TEMP: a reagent used to detect 1O2. DMPO: a reagent used to detect O2•- and ∙OH

图5 不同浓度FePS3 NSs的可见-近红外吸收光谱图(a), 不同浓度(b)和不同激光功率密度(1064 nm激光)(c)条件下FePS3 NSs随时间的光热升温曲线, 以及FePS3 NSs 5次激光开闭循环辐照的温度曲线(d)

Fig. 5 Vis-NIR spectra of FePS3 NSs with different concentrations (a), photothermal heating curves for different time at different concentrations (b), and different laser power densities (1064 nm laser) (c), and photothermal curve of FePS3 NSs under 5 cycles of laser “on-off” (d)

图6 不同浓度FePS3 NSs 在1064 nm激光照射下随时间升温的红外热成像照片

Fig. 6 Thermal images of different concentrations of FePS3 nanosheets heated by 1064 nm laser irradiation for different time

图7 FePS3 NSs 在λ=1064 nm处的归一化吸收强度除以相应浓度下样品特征长度(A/L)与相应浓度的线性拟合曲线(a), FePS3 NSs经1064 nm激光辐照后冷却过程的-lnθ与时间的线性关系(b), FePS3 NSs在1064 nm激光辐照下的升温和冷却曲线及冷却过程的-lnθ与时间的线性关系(c)

Fig. 7 Linear fitting curve between normalized absorption intensity of FePS3 NSs at λ=1064 nm divided by the characteristic length of the sample at corresponding concentration (A/L) and the corresponding concentration (a), linear relationship between -lnθ and time of cooling process of FePS3 NSs after 1064 nm laser irradiation (b), heating and cooling curves of FePS3 NSs under 1064 nm laser irradiation, and linear relationship between -lnθ and time of the cooling process (c) ε, τs and η represent extinction coefficient, time constant in cooling stage, and photothermal conversion efficiency, respectively, of FePS3 NSs under 1064 nm laser irradiation

图8 4T1细胞与不同浓度FePS3-PEG共孵育后的相对活力

Fig. 8 Relative cell viabilities of 4T1 cells after incubation with different concentrations of FePS3-PEG

图9 与不同浓度FePS3-PEG 共孵育后对4T1细胞进行的体外光动力学治疗(a)和光热治疗(b)

Fig. 9 In vitro photodynamic therapy (a) and photothermal therapy (b) treatment of 4T1 cells after incubation with different concentrations of FePS3-PEG

图10 经不同处理后的4T1细胞活力(a)、ROS产物(b)及线粒体膜电位变化(c)的CLSM荧光照片

Fig. 10 Confocal laser scanning microscope (CLSM) images of 4T1 cell viabilities (a), reactive oxygen species (ROS) production (b) and changes in mitochondrial membrane potential (c) after different treatments Calcein-AM/PI, DCFH-DA and JC-1 represent methods for detecting cell viability, ROS production, and changes in mitochondrial membrane potential, respectively